Gene expression profiles of vitrified in vivo derived 8‐cell stage mouse embryos detected by high density oligonucleotide microarrays

Mamo, Solomon; Bodó, Szilárd; Kobolák, Julianna; Polgár, Zsuzsanna; Tölgyesi, Gergely; Dinnyés, András

doi:10.1002/mrd.20588

Cited by 44 publications

(34 citation statements)

References 49 publications

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“…One of the major potential problems with DMSO is that it is a very potent solvent. This permeating cryoprotectant may introduce toxic compounds into the embryos [18,19]. Recent studies have shown that vitrification of cleavage embryos resulted in severe loss of methylation in the H19/Igf2 differentially methylated domain (DMD) [20,21] and down-regulation of the expression of Bax, Bcl2 and P53 genes [22].…”

Section: Discussionmentioning

confidence: 99%

Effect of vitrification versus slow freezing of human day 3 embryos on β-hCG levels

Xue¹,

Jiang²,

Zhu³

et al. 2014

J Assist Reprod Genet

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Purpose The study was designed to investigate the effect of vitrification and slow freezing for the cryopreservation of human day 3 embryos on serum β-hCG levels in pregnancies established after frozen embryo transfer (FET). Methods Of the 1384 FET cycles initiated, 1131 embryo transfers met study criteria and assigned to one of two groups: 797 slow-freezing embryo transfers or 334 vitrified embryo transfers. Median values of β-hCG and outcome of all pregnancies were compared between the two groups. Predictive values of serum β-hCG on day 12 after embryo transfer for establishing ongoing pregnancy and pregnancy failure were determined by receiver operating characteristic (ROC) curve analysis. Results In the slow-freezing group, 383 ongoing pregnancies occurred (48.1 %), and transfers of vitrified embryos resulted in 154 pregnancies (46.1 %). Median β-hCG values (279.2 IU/L) for ongoing pregnancies after transfer of vitrified embryos were significantly lower than that of slow frozen embryos (320.5 IU/L). The median values of β-hCG for singleton in the two groups was statistically significant (P<0.05). For slowfreezing embryo transfers, the cut-off value of β-hCG in predicting ongoing pregnancy was 147 IU/L (sensitivity 88.3 %, specificity 80.7 %). For vitrified embryo transfers, the value was 135 IU/L (sensitivity 84.4 %, specificity 76.3 %). Conclusions Day 12 β-hCG levels after FET are significantly affected by the methods of embryo cryopreservation for ongoing pregnancies. Furthermore, when using β-hCG cutoff value to assess pregnancy outcome, the cryopreservation methods should be taken into account.

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Section: Discussionmentioning

confidence: 99%

Effect of vitrification versus slow freezing of human day 3 embryos on β-hCG levels

Xue¹,

Jiang²,

Zhu³

et al. 2014

J Assist Reprod Genet

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“…Lower CD9 mRNA expression has been observed in vitrified-warmed bovine [91] and ovine [94] oocytes compared with that in non-vitrified oocytes. There are a wide range of consequences resulting from vitrification of mouse embryos, including effects on metabolism and regulation of cellular and physiological activities such as proliferation, the cell cycle, development, biosynthesis, respiration, and stress-related gene expression [107,108]. Interestingly, vitrification causes ma-jor changes in the gene expression of IVF bovine embryos, whereas no major changes are observed in the gene expression of in vivo-derived (IVV) embryos after vitrification [109].…”

Section: Molecular Effects Of Cryopreservationmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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“…In the preimplantation two-cell mouse embryo, IGF-II stimulates cell division and blastocyst formation [32], while ZO-1, one of the peripheral membrane proteins of tight junctions [33], is synthesized late in the four-cell stage [34] and is involved in blastocyst formation from morula by regulating accumulation of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells [35] E-cadherin, an integral membrane component of the adherens junction (first detected at early morula and blastocyst stages [36]), is necessary for the formation of the trophectoderm epithelium since E-cadherin null embryos undergo compaction but fail to form blastocysts containing trophoblast cells [37]. The upregulation of these genes is possibly activated so as to compensate for obesity-related stress that is similar to vitrification-induced stress to the embryo [38]. Although the expression of IGF-II, ZO-1, and Ecadherin was significantly upregulated in embryos from obese mice, their embryonic development was still notably delayed.…”

Section: Discussionmentioning

confidence: 99%

Maternal obesity in mice not only affects fresh embryo quality but also aggravates injury due to vitrification

Huang

Yang

et al. 2016

J Assist Reprod Genet

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Purpose The aims of the present study are to identify the mechanism(s) whereby obesity impairs fresh embryos and to clarify the effects of vitrification on lipid droplet content within embryos from maternally obese mice. Methods The diet-induced obesity mouse model was established, and the zygotes were captured and cultured to day 3. The eight-cell embryos were selected and divided into fresh and vitrified groups. The blastocysts derived from fresh embryos were used as a control. The expression profiles of endoplasmic reticulum (ER) stress genes (Atf4, Grp78, and Hsp70) and other genes (MnSOD, p53, Gadd45g, caspase-3, IGF-II, ZO-1, and E-cadherin) on day-3 fresh and postwarming eight-cell embryos from obese and control groups were determined. For day-5 fresh blastocysts and blastocysts previously vitrified on day 3, the expression profiles for all of the above genes were also determined. Results For the fresh group, obesity significantly upregulated Hsp70, p53, IGF-II, and ZO-1 expression in embryos on day 3 and notably upregulated Atf4, MnSOD, Gadd45g, caspase-3, ZO-1, and E-cadherin expression in blastocysts on day 5. For vitrified ones, obesity significantly upregulated Atf4, MnSOD, and Gadd45g expression in embryos on day 3 and notably upregulated Hsp70 expression and downregulated MnSOD in day 5 blastocysts previously vitrified on day 3. Conclusions Obesity impairs fresh embryos and aggravates embryonic vitrification injury at a molecular level.

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Gene expression profiles of vitrified in vivo derived 8‐cell stage mouse embryos detected by high density oligonucleotide microarrays

Cited by 44 publications

References 49 publications

Effect of vitrification versus slow freezing of human day 3 embryos on β-hCG levels

Effect of vitrification versus slow freezing of human day 3 embryos on β-hCG levels

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Maternal obesity in mice not only affects fresh embryo quality but also aggravates injury due to vitrification

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