Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice

Abstract

The highly expressed genes encoding heat-shock proteins, HSP82 and SSA4, potentially play an important role in helping S. cerevisiae KKU-VN8 cope with various stresses that occur during high-temperature fermentation, leading to higher ethanol production efficiency.

Collagen Peptide Provides Saccharomyces cerevisiae with Robust Stress Tolerance for Enhanced Bioethanol Production

“…The industrial strains of S. cerevisiae ADZ-230, KJB-256, FGL-853, and CXD-833, were selected to gain further insight into the applicability of CP in bioethanol production. In addition, the initial sugar concentrations of 240 mg/mL and 250 mg/mL were generally used in the investigation of ethanol fermentation. , When CP was added into FM24, the residual sugar content at 120 h declined from 39.71 to 0.76 mg/mL, 75.64 to 0.73 mg/mL, and 67.52 to 0.77 mg/mL for S. cerevisiae ADZ-230, KJB-256, and FGL-853, respectively (Figure a1,b1,c1). Moreover, the glucose utilization rate in FM25_2CP increased from 69.22 to 99.65% at 120 h for S. cerevisiae CXD-833, compared with that in FM25 (Figure d1).…”

“…According to the method of Wang et al, the qRT-PCR was performed using 96 well plates on a Step One Plus Real-Time PCR instrument (Applied Biosystems, Foster, CA, USA). ACT1 (actin), a housekeeping gene, was used as an internal control to normalize the PCRs for the amount of RNA added to the reactions. , The relative changes in the gene expression level were calculated using the 2 –ΔΔCt method. , The primers used in the qRT-PCR assay were synthesized by Sangon (Sangon Biotech, China) and are listed in Table S1.…”

“…ACT1 (actin), a housekeeping gene, was used as an internal control to normalize the PCRs for the amount of RNA added to the reactions. 39,40 The relative changes in the gene expression level were calculated using the 2 −ΔΔCt method. 41,42 The primers used in the qRT-PCR assay were synthesized by Sangon (Sangon Biotech, China) and are listed in Table S1.…”

Saccharomyces cerevisiae biomass as a source of next‐generation food preservatives: Evaluating potential proteins as a source of antimicrobial peptides

“…Heat shock proteins encoded by HSP genes, such as HSP26 , HSP82 , HSP10 , HSP12 , play a general cell response to abiotic stresses such as thermal and oxidative stress, ethanol exposure, nutrient deprivation, or high osmolarity experienced by yeast cells during biomass propagation (Figure 5). HSPs contribute to maintain yeast viability by protecting the main glycolysis enzymes, pyruvate kinase and hexokinase denaturation, and other essential proteins, as the consecutive stresses during biomass propagation affect not only the conformation of existing proteins but also the folding of newly synthesized polypeptide chains, the prevention of irreversible protein denaturation and/or aggregation, denatured cellular protein refolding, the degradation of incorrectly folded proteins and protection of newly synthetized ones (Alexandre et al., 2001; Auesukaree et al., 2009; Fujita et al., 2004; Techaparin et al., 2017). HSP‐related genes like HSC82 , SSC1 , STI1 , and SBA1 can also be up‐regulated in such stress conditions (Table S1) (Verghese et al., 2012; Święciło, 2016).…”

Characterization of a thermo-adapted strain of Zymomonas mobilis for ethanol production at high temperature

“…The benefits also include a high bioconversion rate, facilitated product recovery, and more efficient simultaneous saccharification and fermentation (SSF). However, high temperature during the fermentation process may inhibit cell growth and hamper cell viability, resulting in a reduction of ethanol yield (Thanonkeo et al 2007;Sootsuwan et al 2013;Techaparin et al 2017). Therefore, highly thermotolerant microorganisms are essential for steady fermentation.…”