Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9

Abstract

ABSTRACT.A strain of the microalga Chlorella pyrenoidosa F-9 in our laboratory showed special characteristics when transferred from autotrophic to heterotrophic culture. In order to elucidate the possible metabolic mechanism, the gene expression profiles of the autonomous organelles in the green alga C. pyrenoidosa under autotrophic and heterotrophic cultivation were compared by suppression subtractive hybridization technology. Two subtracted libraries of autotrophic and heterotrophic C. pyrenoidosa F-9 were constructed, and 160 clones from the heterotrophic library were randomly selected for DNA sequencing. Dot blot hybridization showed that the ratio of positivity was 70.31% from the 768 clones. Five chloroplast genes (ftsH, psbB, rbcL, atpB, and infA) and two mitochondrial genes (cox2 and nad6) were selected to verify their expression levels by real-time quantitative polymerase chain reaction. Results showed that the seven genes were abundantly expressed in the heterotrophic culture. Among the seven genes, the least increment of gene expression was ftsH, which was expressed 1.31-1.85-fold higher under heterotrophy culture than under autotrophy culture, and the highest increment was psbB, which increased 28.07-39.36 times compared with that under autotrophy conditions. The expression levels of the other five genes were about 10 times higher in heterotrophic algae than in autotrophic algae. In inclusion, the chloroplast and mitochondrial genes in C. pyrenoidosa F-9 might be actively involved in heterotrophic metabolism.

Use of De Novo Transcriptome Libraries to Characterize a Novel Oleaginous Marine Chlorella Species during the Accumulation of Triacylglycerols

“…Most previous transcriptomic studies of various Chlorella species utilized targeted transcriptomic approaches such as reverse-transcription quantitative PCR (RT-qPCR) to monitor pre-selected targets [ 23 – 25 ]. However, analyses of the full transcriptome using next-generation sequencing technologies are more useful because they can provide a global overview of the response of Chlorella cultures to various environmental states, and potentially link the expression of specific and large sets of transcripts with phenotypic responses.…”