Gene Expression Profiles of Peripheral Blood Monocytes in Osteoarthritis and Analysis of Differentially Expressed Genes

Abstract

Background There is little understanding of the molecular processes involved in the pathogenesis of osteoarthritis, limiting early diagnosis and effective treatment of OA. Use of genechips can provide insights into the molecular pathogenesis of diseases. In this study, determination of gene expression profiles of osteoarthritis peripheral blood mononuclear cells will allow exploration of the molecular pathogenesis of OA and find out more candidate biomarkers and potential drug targets of OA. Result A total of 1231 DEGs were screened out including 791 upregulated DEGs and 440 downregulated DEGs. The most significant upregulated DEG was RPL38, which may inhibit chondrocyte differentiation and synthesis of the extracellular matrix. PIK3CA, PIK3CB, PIK3CD, PIK3R1, MAPK14, IL1A, JUND, FOSL2, and PPP3CA were the gene symbols of the osteoclast differentiation pathway which was the most significant pathway enriched by DEGs. However, the MAPK signaling pathway occupied the core position of all the pathways which can regulate apoptosis, cell cycle, wnt signaling pathway, p53 signaling pathway, and phosphatidylinositol signaling system. Furthermore, PI3Ks may regulate IL1A, JUND, FOSL2 and PPP3CA through the MAPK signaling pathway. Conclusion These identified DEGs and pathways may be novel biomarkers to monitor the changes of OA and can be a potential drug target for the treatment of OA.

Single-cell RNA landscape of intratumoral heterogeneity and immunosuppressive microenvironment in advanced osteosarcoma

“…Conversely, some well-known factors such as NFATC1, SPI1 , and FOSL2 were upregulated in the process (Fig. 4f ), which are involved in regulating differentiation, survival and size of OC 37 , 38 . We also found some unidentified regulators such as JDP2, ZNF267, CAMTA2, MLX, HES4, and GLMP in OS lesions, which are potentially engaged in the cellular transition from the myeloid monocytes into mature OC cells (Fig.…”

An Investigation of the Molecular Mechanisms Underlying the Analgesic Effect of Jakyak‐Gamcho Decoction: A Network Pharmacology Study

“…ese hubs were shown to be involved in the regulation of pain-related processes and could function as potent targets to induce analgesic effects. e PIK3R1 gene was suggested to have the potential to function as a painrelated regulator according to the genetic interaction analysis [133], and its expression level might be associated with osteoarthritis pathogenesis [134]. Upregulation of the HSP90AA1 gene was observed in patients with fibromyalgia [135][136][137], and pharmacological inhibition of heat shock protein 90 (HSP90; encoded by HSP90AA1) was shown to alleviate monoarthritis-induced pain [138].…”

Investigating the active substance and mechanism of Jing-Fu-Kang granules via mass spectrometry technology and network pharmacology method

“…the evaluation and control of the quality of traditional Chinese medicine must truly reflect its "internal" and "comprehensive" quality [26]. [30]; and its expression level may be related to the pathogenesis of osteoarthritis [31];…”

“…inflammatory reaction and regulate immune function to treat CS. According to the analysis of genetic interaction; PIK3R1 gene is considered to have a potential pain-related regulatory function [30]; and its expression level may be related to the pathogenesis of osteoarthritis [31]; which lays a foundation for the pharmacological effect of JFKG on promoting blood circulation and relieving pain. Pharmacological data in previous research showed that PI3K-Akt signal pathway can promote angiogenesis and promote blood flow by activating important vasoactive factors such as vascular endothelial growth factor and nitric oxide [32][33].…”