Gene expression profiles of glucose toxicity‐exposed islet microvascular endothelial cells

Liu, Mingming; Lu, Wenbao; Hou, Qunxing; Wang, Bing; Sheng, Youming; Wu, Qiliang; Li, Bingwei; Liu, Xueting; Zhang, Xiaoyan; Li, Ailing; Zhang, Honggang; Xiu, Ruijuan

doi:10.1111/micc.12450

Cited by 3 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liu et al ( 161 ) determined the effects of glucotoxicity on gene expression in pancreatic islet microvascular endothelial cells (IMECs) by treating them with 5.6 mmol L-1 glucose (control group), 35 mmol L-1 glucose (glucotoxicity), and 35 mmol L-1 glucose plus 10-8 mol L-1 insulin. The study group determined that glucotoxicity resulted in the differential expression of 1,574 mRNAs compared to the control and 2,870 mRNAs relative to the insulin-treated group.…”

Section: Discussionmentioning

confidence: 99%

“…The study group determined that glucotoxicity resulted in the differential expression of 1,574 mRNAs compared to the control and 2,870 mRNAs relative to the insulin-treated group. In addition, they also identified that these deregulated genes played roles in the regulation of proliferation, apoptosis, adhesion, migration, and metabolic activities ( 161 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular pathways and nutrigenomic review of insulin resistance development in gestational diabetes mellitus

Guevara-Ramírez,

Paz-Cruz,

Cadena-Ullauri

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

Gestational diabetes mellitus is a condition marked by raised blood sugar levels and insulin resistance that usually occurs during the second or third trimester of pregnancy. According to the World Health Organization, hyperglycemia affects 16.9% of pregnancies worldwide. Dietary changes are the primarily alternative treatment for gestational diabetes mellitus. This paper aims to perform an exhaustive overview of the interaction between diet, gene expression, and the metabolic pathways related to insulin resistance. The intake of foods rich in carbohydrates can influence the gene expression of glycolysis, as well as foods rich in fat, can disrupt the beta-oxidation and ketogenesis pathways. Furthermore, vitamins and minerals are related to inflammatory processes regulated by the TLR4/NF-κB and one carbon metabolic pathways. We indicate that diet regulated gene expression of PPARα, NOS, CREB3L3, IRS, and CPT I, altering cellular physiological mechanisms and thus increasing or decreasing the risk of gestational diabetes. The alteration of gene expression can cause inflammation, inhibition of fatty acid transport, or on the contrary help in the modulation of ketogenesis, improve insulin sensitivity, attenuate the effects of glucotoxicity, and others. Therefore, it is critical to comprehend the metabolic changes of pregnant women with gestational diabetes mellitus, to determine nutrients that help in the prevention and treatment of insulin resistance and its long-term consequences.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular pathways and nutrigenomic review of insulin resistance development in gestational diabetes mellitus

Guevara-Ramírez,

Paz-Cruz,

Cadena-Ullauri

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…Glucose-mediated modulation of histone methylation has also been linked to TGF-β-dependent promoters and expression of ECM-associated genes [ 13 ]. mRNA profiling by microarray analyses of mesangial and other cell types treated with elevated glucose conditions has shown changes in expected pathways like cell proliferation and metabolism, but also in pathways related to the cytoskeleton and focal adhesions [ 14 , 15 ]. The effects of glucose levels even extend to changes in alternative splicing of certain transcripts, including growth factors, their receptors, and FN [ [16] , [17] , [18] , [19] ].…”

Section: Introductionmentioning

confidence: 99%

Elevated glucose alters global gene expression and tenascin-C alternative splicing in mesangial cells

Vega

Finlay

Vasishtha

et al. 2020

Matrix Biology Plus

View full text Add to dashboard Cite

Mesangial cells are the major extracellular matrix (ECM)-producing cells in the kidney glomerulus and, when exposed to elevated glucose levels, they up-regulate assembly of fibronectin (FN) and other ECM proteins. Increases in glucose concentration are known to alter gene expression; here we investigated the connection between increased ECM production and changes in gene expression in mesangial cells. Comparison of mesangial cells grown in normal or high glucose conditions by RNA-sequencing showed significant expression changes in over 6000 genes and, when grouped by KEGG pathway analysis, identified the ECM-receptor interaction and focal adhesion pathways among the top 5 upregulated pathways. Of note was the significant increase in expression of tenascin-C (TN-C), a known regulator of FN matrix assembly. Mouse TN-C has multiple isoforms due to alternative splicing of 6 FNIII repeat exons. In addition to the transcriptional increase with high glucose, exon inclusion via alternative splicing was also changed resulting in production of higher molecular weight isoforms of TN-C. Mesangial cells grown in normal glucose secreted small isoforms with 1–2 variable repeats included whereas in high glucose large isoforms estimated to include 5 repeats were secreted. Unlike the smaller isoforms, the larger TN-C was not detected in the FN matrix. This change in TN-C isoforms may affect the regulation of FN matrix assembly and in this way may contribute to increased ECM accumulation under high glucose conditions.

show abstract

Harnessing the benefits of glycine supplementation for improved pancreatic microcirculation in type 1 diabetes mellitus

Wang,

Song,

Zhang

et al. 2024

Microvascular Research

View full text Add to dashboard Cite

Gene expression profiles of glucose toxicity‐exposed islet microvascular endothelial cells

Abstract: Our study provides a microcirculatory framework for gene expression profiles of glucose toxicity-exposed IMECs.

Cited by 3 publications

References 41 publications

Molecular pathways and nutrigenomic review of insulin resistance development in gestational diabetes mellitus

Molecular pathways and nutrigenomic review of insulin resistance development in gestational diabetes mellitus

Elevated glucose alters global gene expression and tenascin-C alternative splicing in mesangial cells

Harnessing the benefits of glycine supplementation for improved pancreatic microcirculation in type 1 diabetes mellitus

Contact Info

Product

Resources

About