Gene expression profiles of four heat shock proteins in response to different acute stresses in shrimp, Litopenaeus vannamei

Qian, Zhaoying; Liu, Xiaolin; Wang, Lijun; Wang, Xian-Zong; Li, Yang; Xiang, Jianhai; Wang, Ping

doi:10.1016/j.cbpc.2012.06.001

Cited by 84 publications

(54 citation statements)

References 46 publications

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“…Heat shock proteins (Hsp) are highly conserved proteins well known for their quick responses to stress (Qian et al, 2012). When induced by environmental stress, Hsp70s work to repair partially denatured proteins, facilitate the degradation of irreversibly denatured proteins, and inhibit protein aggregation (Parsell & Lindquist, 1993).…”

Section: __________________ Corresponding Editor: Fernando Vegamentioning

confidence: 99%

Effects of bacilli, molasses, and reducing feeding rate on biofloc formation, growth, and gene expression in Litopenaeus vannamei cultured with zero water exchange

Luna‐González

Ávila-Leal

Fierro-Coronado

et al. 2017

lajar

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ABSTRACT. The effects of reducing feeding rate and molasses on water quality, growth, and gene expression of Litopenaeus vannamei were evaluated in a super intensive system with zero water exchange. A bioassay of 35 days (0,75 ± 0,22 g, 36 shrimp tank ) with treatments in triplicate was performed: I) 100% commercial feed (control group) with water exchange; II) 100% commercial feed without water exchange; III) 100% commercial feed + Bacillus licheniformis BCR 4-3 + molasses without water exchange; IV) 90% commercial feed + B. licheniformis BCR 4-3 + molasses without water exchange, and V) 80% commercial feed + B. licheniformis BCR 4-3 + molasses without water exchange. Shrimp survival, weight, nitrogenous wastes, settleable solids (SS), and the relative expression (RT-qPCR) of stress and digestive genes were determined. Shrimp survival was 100% in all treatments. The reduced diet, bioflocs, and bacilli did not affect shrimp growth. The culture conditions reduced nitrogenous wastes and modulated mRNA expression of trypsin, chymotrypsin, and heat shock protein 70. Reduced feed ration can help to maintain water quality and lower costs in intensive shrimp cultures with bioflocs.

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Section: __________________ Corresponding Editor: Fernando Vegamentioning

confidence: 99%

Effects of bacilli, molasses, and reducing feeding rate on biofloc formation, growth, and gene expression in Litopenaeus vannamei cultured with zero water exchange

Luna‐González

Ávila-Leal

Fierro-Coronado

et al. 2017

lajar

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“…Many papers describe its toxic effect on DNA and the damage to and inhibition of relative repair mechanisms (Emmanouil et al, 2007;Joseph, 2009;Pruski and Dixon, 2002), and the general reduction of protein synthesis coupled with an increase in stress proteins (Qian et al, 2012;Said Ali et al, 2010). Cadmium, whose effects in sublethal concentrations on fish metabolism are well known, has been shown to alter the structure and to cause morpho-pathological changes of varying severity in various organs (Atif et al, 2005;Thophon et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tThe immunotoxic effects of heavy metals on blood leukocytes of sea bass (Dicentrarchus labrax) were examined. The cells, separated by a discontinuous Percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10 À 7 M, 10 À 5 M, and 10 À 3 M) and their immunotoxic effect was then evaluated by measuring neutral red uptake, MTT assay, DNA fragmentation and Hsp70 gene expression. First of all, we demonstrated that the cells treated in vitro could incorporate Cd and Cu. A relationship between heavy metal exposure and dose-time-dependent alterations in responses of leukocytes from blood was found for both metals, but copper was more immunotoxic than cadmium in all assays performed. A significant reduction in the cells' ability to uptake neutral red and viability by MTT assay was recorded, indicating that both cadmium and copper could change the membrane permeability, inducing cellular apoptosis when the concentration of metals reached 10 À 3 M. The apoptotic effect may also explain the high level of cytotoxicity found when the leukocytes were exposed to higher concentration of metals. These results demonstrated that toxic effect of copper and cadmium affect on the mechanisms of cellmediated immunity reducing the immune defences of the organism.

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“…Considering the enormous economic losses induced by environmental stressors in the shrimp industry, studies focusing on heat shock proteins have become increasingly popular because of the important roles these proteins play in resistance to stress (Cesar and Yang 2007;Cui et al 2010;Li et al 2012;Qian et al 2012). Unfortunately, only three Hsps, namely, Hsp90, Hsp70, and Hsp21, have been reported in P. monodon (Lo et al 2004;Huang et al 2008;Jiang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Hsp60 is able to substantially accelerate procaspase-3 maturation by different upstream activator caspases (Xanthoudakis et al 1999). The possible roles of Hsp60 in certain cellular processes, such as sperm cell differentiation, reproduction development, and environment stress response, have also been elucidated by other researches (Meinhardt et al 1995;Paranko et al 1996;Kozlova et al 1997;Neuer et al 2000;Qian et al 2012). Much of the research on organism protection under environmental stress in the last decades has examined the involvement of Hsp60 in multiple pathophysiological processes.…”

Section: Introductionmentioning

confidence: 99%

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

Shi

Zhao

et al. 2016

Cell Stress and Chaperones

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Heat shock proteins (Hsps) are a class of highly conserved proteins produced in virtually all living organisms from bacteria to humans. Hsp60 and Hsp10, the most important mitochondrial chaperones, participate in environmental stress responses. In this study, the full-length complementary DNAs (cDNAs) of Hsp60 (PmHsp60) and Hsp10 (PmHsp10) were cloned from Penaeus monodon. Sequence analysis showed that PmHsp60 and PmHsp10 encoded polypeptides of 578 and 102 amino acids, respectively. The expression profiles of PmHsp60 and PmHsp10 were detected in the gills and hepatopancreas of the shrimps under pH challenge, osmotic stress, and heavy metal exposure, and results suggested that PmHsp60 and PmHsp10 were involved in the responses to these stimuli. ATPase and chaperone activity assay indicated that PmHsp60 could slow down protein denaturation and that Hsp60/Hsp10 may be combined to produce a chaperone complex with effective chaperone and ATPase activities. Overall, this study provides useful information to help further understand the functional mechanisms of the environmental stress responses of Hsp60 and Hsp10 in shrimp.

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Gene expression profiles of four heat shock proteins in response to different acute stresses in shrimp, Litopenaeus vannamei

Cited by 84 publications

References 46 publications

Effects of bacilli, molasses, and reducing feeding rate on biofloc formation, growth, and gene expression in Litopenaeus vannamei cultured with zero water exchange

Effects of bacilli, molasses, and reducing feeding rate on biofloc formation, growth, and gene expression in Litopenaeus vannamei cultured with zero water exchange

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

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