Gene expression profiles in the articular cartilage of juvenile rats receiving the quinolone antibacterial agent ofloxacin

Goto, Kōichi; Yabe, Koichi; Suzuki, Takami; Takasuna, Kiyoshi; Jindo, Toshimasa; Manabe, Sunao

doi:10.1016/j.tox.2008.05.005

Cited by 19 publications

(16 citation statements)

References 37 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However limited attention has been given to the intrapartum use of antibiotics and subsequent health outcomes [13]. Antibiotics have been shown to trigger altered gene expression and intestinal microbiota in rats, influencing immune system development and function [95,96]. The influence of fetal/neonatal exposure to antibiotics on development of immune dysfunction may initiate a cascade of events associated with future health conditions unexplained by the acute response of altered intestinal flora induced by maternal antibiotic exposure during a susceptible period [97].…”

Section: Evaluation Of the Hypothesismentioning

confidence: 99%

The EPIIC hypothesis: Intrapartum effects on the neonatal epigenome and consequent health outcomes

et al. 2013

View full text Add to dashboard Cite

There are many published studies about the epigenetic effects of the prenatal and infant periods on health outcomes. However, there is very little knowledge regarding the effects of the intrapartum period (labor and birth) on health and epigenetic remodeling. Although the intrapartum period is relatively short compared to the complete perinatal period, there is emerging evidence that this time frame may be a critical formative phase for the human genome. Given the debates from the National Institutes of Health and World Health Organization regarding routine childbirth procedures, it is essential to establish the state of the science concerning normal intrapartum epigenetic physiology. EPIIC (Epigenetic Impact of Childbirth) is an international, interdisciplinary research collaboration with expertise in the fields of genetics, physiology, developmental biology, epidemiology, medicine, midwifery, and nursing. We hypothesize that events during the intrapartum period – specifically the use of synthetic oxytocin, antibiotics, and cesarean section – affect the epigenetic remodeling processes and subsequent health of the mother and offspring. The rationale for this hypothesis is based on recent evidence and current best practice.

show abstract

Section: Evaluation Of the Hypothesismentioning

confidence: 99%

The EPIIC hypothesis: Intrapartum effects on the neonatal epigenome and consequent health outcomes

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Even more common are ruptures of the shoulder, hand, Achilles, or other tendons that require surgical repair or result in prolonged disability [157]. Interestingly, extensive changes in gene expression were found in articular cartilage of rats receiving the quinolone antibacterial agent ofloxacin, suggesting a potential epigenetic mechanism for the arthropathy caused by these agents [158]. It has also been documented that the incidence of hepatic and dysrhythmic cardiovascular events following use of fluoroquinolones is increased compared to controls, suggesting the possibility of persistent gene expression changes in the liver and heart [159].…”

Section: Chloroquine and Fluoroquinolone Antibioticsmentioning

confidence: 99%

Epigenetic side-effects of common pharmaceuticals: A potential new field in medicine and pharmacology

2009

View full text Add to dashboard Cite

“…Furthermore, topical ciprofloxacin increased the incidence of corneal perforations, significantly delaying corneal wound healing (4,5) and in a separate study prolonged tympanic membrane perforation healing (6). Increased matrix metalloproteinase (MMP) synthesis in response to ciprofloxacin treatment has been reported in several cell types, including tenocytes (7–10), chondrocytes (11), corneal epithelial cells and corneal stromal keratocytes (4). …”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin has antifibrotic effects in scleroderma fibroblasts via downregulation of Dnmt1 and upregulation of Fli1

Bujor

Haines

Padilla

et al. 2012

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Systemic sclerosis (SSc) is characterized by fibrosis of the skin and internal organs. The present study was undertaken to examine the effects of ciprofloxacin, a fluoroquinolone antibiotic implicated in matrix remodeling, on dermal and lung fibroblasts obtained from SSc patients. Dermal and lung fibroblasts from SSc patients and healthy subjects were treated with ciprofloxacin. Western blotting was used to analyze protein levels and RT-PCR was used to measure mRNA expression. The pharmacologic inhibitor UO126 was used to block Erk1/2 signaling. SSc dermal fibroblasts demonstrated a significant decrease in collagen type I mRNA and protein levels after antibiotic treatment, while healthy dermal fibroblasts were less sensitive to ciprofloxacin, downregulating collagen only at the protein levels. Connective tissue growth factor (CCN2) gene expression was significantly reduced and matrix metalloproteinase 1 (MMP1) levels were enhanced after ciprofloxacin treatment to a similar extent in healthy and SSc fibroblasts. Ciprofloxacin induced Erk1/2 phosphorylation, and Erk1/2 blockade completely prevented MMP1 upregulation. However, Smad1 and Smad3 activation in response to TGFβ was not affected. The expression of friend leukemia integration factor 1 (Fli1), a transcriptional repressor of collagen, was increased after treatment with ciprofloxacin only in SSc fibroblasts, and this was accompanied by a decrease in the levels of DNA methyltransferase 1 (Dnmt1). Similar effects were observed in SSc-interstitial lung disease (ILD) lung fibroblasts. In summary, our study demonstrates that ciprofloxacin has antifibrotic actions in SSc dermal and lung fibroblasts via the downregulation of Dnmt1, the upregulation of Fli1 and induction of MMP1 gene expression via an Erk1/2-dependent mechanism. Thus, our data suggest that ciprofloxacin may be an attractive therapy for SSc skin and lung fibrosis.

show abstract

Gene expression profiles in the articular cartilage of juvenile rats receiving the quinolone antibacterial agent ofloxacin

Cited by 19 publications

References 37 publications

The EPIIC hypothesis: Intrapartum effects on the neonatal epigenome and consequent health outcomes

The EPIIC hypothesis: Intrapartum effects on the neonatal epigenome and consequent health outcomes

Epigenetic side-effects of common pharmaceuticals: A potential new field in medicine and pharmacology

Ciprofloxacin has antifibrotic effects in scleroderma fibroblasts via downregulation of Dnmt1 and upregulation of Fli1

Contact Info

Product

Resources

About