Gene expression profiles during differentiation and transdifferentiation of bovine myogenic satellite cells

Lee, Eun Ju; Bajracharya, Prati; Lee, Dong-Mok; Kang, Se Won; Lee, Yong Seok; Lee, Hyun-Jeong; Hong, Sung Kee; Chang, Jong-Soo; Kim, Jae Woo; Schnabel, Robert D.; Taylor, Jeremy F.; Choi, Inho

doi:10.1007/s13258-011-0096-z

Cited by 13 publications

(19 citation statements)

References 26 publications

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“…Consistent with our previous study investigating up-regulation of AOX1 during the myogenic differentiation of bovine satellite cells [8,9], we report here significant up-regulation of AOX1 during the differentiation of mouse-derived C2C12 cells that has been widely used to study myogenesis [17]. This study revealed that AOX1 knock-down significantly reduces myotube formation and suppresses the expression of all tested MRFs, thereby clearly indicating that AOX1 is, at least in part, involved in the regulation of myogenesis, although its physiological role and action mechanism are yet to be elucidated.…”

Section: Discussionsupporting

confidence: 93%

“…Maintaining a balance between activation, proliferation and differentiation of quiescent MSCs within the muscle requires the involvement of various myogenic regulatory factors (MRFs), including several transcription factors such as MYF5, MYOD, MRF4 and myogenin (MYOG), which regulate the expression of target genes during myogensis [7]. In previous functional studies of MSCs, we found several genes that were up-regulated, including the gene encoding aldehyde oxidase 1 (AOX1), which has no known function in myogenesis [8,9]. AOX1, an enzyme belonging to the molybdoflavoenzyme (MOFEs) family, which require flavin adenine dinucleotide (FAD) and molybdopterin cofactor for their catalytic activity, shows a high degree of conservation throughout the animal kingdom [10].…”

Section: Introductionmentioning

confidence: 99%

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Expressional studies of the aldehyde oxidase (AOX1) gene during myogenic differentiation in C2C12 cells

Kamli

Kim

Pokharel

et al. 2014

Biochemical and Biophysical Research Communications

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Expressional studies of the aldehyde oxidase (AOX1) gene during myogenic differentiation in C2C12 cells

Kamli

Kim

Pokharel

et al. 2014

Biochemical and Biophysical Research Communications

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“…DNA microarray analyses for FMOD wt vs. FMOD kd C2C12 cells were performed to identify differentially expressed genes (DEGs). DNA microarray analysis was performed as previously described (25). Briefly,…”

Section: Methodsmentioning

confidence: 99%

Fibromodulin and regulation of the intricate balance between myoblast differentiation to myocytes or adipocyte‐like cells

et al. 2018

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Interactions between myoblasts and the surrounding microenvironment led us to explore the role of fibromodulin (FMOD), an extracellular matrix protein, in the maintenance of myoblast stemness and function. Microarray analysis of FMOD kd myoblasts and in silico studies were used to identify the top most differentially expressed genes in FMOD kd , and helped establish that FMOD-based regulations of integral membrane protein 2a and clusterin are essential components of the myogenic program. Studies in knockout, obese, and diabetic mouse models helped characterize the operation of a novel FMOD-based regulatory circuit that controls myoblast switching from a myogenic to a lipid accumulation fate. FMOD regulation of myoblasts is an essential part of the myogenic program, and it offers opportunities for the development of therapeutics for the treatment of different muscle

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“…Similarly, MSCs switched to transdifferentiation media on day 10 were harvested on day 17 for ALC. Total RNA extraction and mRNA purification were conducted according to Lee et al [11]. Briefly, equal amounts of RNA samples collected from cultured MSCs, MFCs and ALCs were pooled together, after which mRNA was purified from each pool of total RNA (100 μg) using the absolutely mRNA purification kit (Stratagene, CA, USA) and used for subsequent library construction.…”

Section: Methodsmentioning

confidence: 99%

“…However, unlike muscle cell differentiation, studies of MSCs transdifferentiation into ALCs are limited and this process is still a matter of debate. Investigations of mouse [4,5,11] and human myoblasts [12] have been carried out to understand the basic mechanism involved in the switch towards ALC formation. …”

Section: Introductionmentioning

confidence: 99%

Expressed Sequence Tags for Bovine Muscle Satellite Cells, Myotube Formed-Cells and Adipocyte-Like Cells

et al. 2013

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BackgroundMuscle satellite cells (MSCs) represent a devoted stem cell population that is responsible for postnatal muscle growth and skeletal muscle regeneration. An important characteristic of MSCs is that they encompass multi potential mesenchymal stem cell activity and are able to differentiate into myocytes and adipocytes. To achieve a global view of the genes differentially expressed in MSCs, myotube formed-cells (MFCs) and adipocyte-like cells (ALCs), we performed large-scale EST sequencing of normalized cDNA libraries developed from bovine MSCs.ResultsA total of 24,192 clones were assembled into 3,333 clusters, 5,517 singletons and 3,842contigs. Functional annotation of these unigenes revealed that a large portion of the differentially expressed genes are involved in cellular and signaling processes. Database for Annotation, Visualization and Integrated Discovery (DAVID) functional analysis of three subsets of highly expressed gene lists (MSC233, MFC258, and ALC248) highlighted some common and unique biological processes among MSC, MFC and ALC. Additionally, genes that may be specific to MSC, MFC and ALC are reported here, and the role of dimethylarginine dimethylaminohydrolase2 (DDAH2) during myogenesis and hemoglobin subunit alpha2 (HBA2) during transdifferentiation in C2C12 were assayed as a case study. DDAH2 was up-regulated during myognesis and knockdown of DDAH2 by siRNA significantly decreased myogenin (MYOG) expression corresponding with the slight change in cell morphology. In contrast, HBA2 was up-regulated during ALC formation and resulted in decreased intracellular lipid accumulation and CD36 mRNA expression upon knockdown assay.ConclusionIn this study, a large number of EST sequences were generated from the MSC, MFC and ALC. Overall, the collection of ESTs generated in this study provides a starting point for the identification of novel genes involved in MFC and ALC formation, which in turn offers a fundamental resource to enable better understanding of the mechanism of muscle differentiation and transdifferentiation.

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Gene expression profiles during differentiation and transdifferentiation of bovine myogenic satellite cells

Cited by 13 publications

References 26 publications

Expressional studies of the aldehyde oxidase (AOX1) gene during myogenic differentiation in C2C12 cells

Expressional studies of the aldehyde oxidase (AOX1) gene during myogenic differentiation in C2C12 cells

Fibromodulin and regulation of the intricate balance between myoblast differentiation to myocytes or adipocyte‐like cells

Expressed Sequence Tags for Bovine Muscle Satellite Cells, Myotube Formed-Cells and Adipocyte-Like Cells

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