Gene Expression Profile of the Hippocampus of Rats Subjected to Chronic Immobilization Stress

Li, Xiaohong; Chen, Jiaxu; Yue, Guang-Xin; Liu, Yueyun; Zhao, Xinyang; Xia-zhen, Guo; Li, Qun; Jiang, You-Ming; Bai, Ming

doi:10.1371/journal.pone.0057621

Cited by 33 publications

(33 citation statements)

References 83 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the chronic stress processed, the combined action of polygenic, multilevel, and multisignal pathways leads to the disorder of the immunologic functions of the hippocampus, hippocampal apoptosis, and proliferation disequilibrium [32]. This is consistent with the protective effect of microstructure of CNQX, and XYS decoction downregulation of GluR1 and upregulation of GluR2 in hippocampus CA 1 respond to the 21-day stress in the present study.…”

Section: Discussionsupporting

confidence: 88%

Effects of the Chinese Traditional Prescription Xiaoyaosan Decoction on Chronic Immobilization Stress-Induced Changes in Behavior and Ultrastructure in Rat Hippocampus

Liang

Xia-zhen

Chen

et al. 2013

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Xiaoyaosan (XYS) decoction has been widely used as a traditional medicine for treating stress and depression-related disorders in China for thousands of years. Aim of the Study. To observe the potential mechanism of XYS decoction's antidepressant-like effect in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors related to synaptic plasticity in the hippocampus rats induced by chronic immobilization stress (CIS). Materials and Methods. Animals were randomly divided into five groups: (1) control group; (2) sham-operated group; (3) CIS group, in which rats were conducted CIS for 21 days; (4) XYS decoction treatment group; (5) 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) positive group, in which the amygdala of CIS rats was unilaterally microinjected with a competitive glutamate receptor antagonist, CNQX. After CIS for 21 days, the open field test (OPT) and elevated plus-maze test (EPM) were measured, the ultrastructure of hippocampus CA1 subregion was observed by the electron microscopy; both the GluR1 and GluR2 mRNA level of AMPA receptor subunits in hippocampus CA1 subregion were detected by real-time qPCR. Results. Rats subjected to CIS exhibited increases in time in central zone and decreases in total distance traveled in the OPT. In the EPM, they also showed decreases in center zone time and entries, open arm time and entries, and an increase in close arm time. Ultrastructural damage in the hippocampus CA1 was also observed. XYS decoction and CNQX showed significant improvement behavioral changes and the ultrastructural damage of the hippocampus CA1; XYS decoction also reversed CIS-induced decreases in GluR2 mRNA and increases in GluR1 mRNA in the hippocampus CA1 as well as CNQX. Conclusions. XYS decoction may effectively produce an antidepressant-like effect, which appears to be involved AMPA receptors related synaptic plasticity of hippocampus.

show abstract

Section: Discussionsupporting

confidence: 88%

Effects of the Chinese Traditional Prescription Xiaoyaosan Decoction on Chronic Immobilization Stress-Induced Changes in Behavior and Ultrastructure in Rat Hippocampus

Liang

Xia-zhen

Chen

et al. 2013

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Differentially expressed genes in the RSS group, such as Plin4 , have been linked to differences in glucocorticoid receptor regulation in relation to anxiety-like behavior and social stress (Stankiewicz, Goscik, Majewska, Swiergiel, & Juszczak, 2015). Additionally, differentially expressed genes in the RSE group such as Adcy8 , BC006965 , Luc7l2 , Kcnh , and Tsc22d3 have been shown to be linked to changes in gene expression after exposure to multiple types of chronic or other kinds of stress and differential gene expression in response to stress across different genetic animal models (Li et al, 2013; Stankiewicz et al, 2014; Szklarczyk, Korostynski, Golda, Solecki, & Przewlocki, 2012; Tordera et al, 2011; Tsolakidou et al, 2010). Over 30 of the differentially expressed genes from the NOE, RSE, or RSE vs. NOE group have been reported as involved in alcohol-related behaviors or expression changes in relation to alcohol exposure.…”

Section: Discussionmentioning

confidence: 99%

Analyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice

Baker

Zhou

et al. 2017

Alcohol

View full text Add to dashboard Cite

Alcohol abuse is a complex disorder, which is confounded by other factors, including stress. In the present study, we examined gene expression in the hippocampus of BXD recombinant inbred mice after exposure to ethanol (NOE), stress (RSS), and the combination of both (RSE). Mice were given an intra-peritoneal (i.p.) injection of 1.8 g/kg ethanol or saline, and subsets of both groups were exposed to acute restraint stress for 15 min or controls. Gene expression in the hippocampus was examined using microarray analysis. Genes that were significantly (p < 0.05, q < 0.1) differentially expressed were further evaluated. Bioinformatic analyses were predominantly performed using tools available at GeneNetwork. org, and included gene ontology, presence of cis-regulation or polymorphisms, phenotype correlations, and principal component analyses. Comparisons of differential gene expression between groups showed little overlap. Gene Ontology demonstrated distinct biological processes in each group with the combined exposure (RSE) being unique from either the ethanol (NOE) or stress (RSS) group, suggesting that the interaction between these variables is mediated through diverse molecular pathways. This supports the hypothesis that exposure to stress alters ethanol-induced gene expression changes and that exposure to alcohol alters stress-induced gene expression changes. Behavior was profiled in all groups following treatment, and many of the differentially expressed genes are correlated with behavioral variation within experimental groups. Interestingly, in each group several genes were correlated with the same phenotype, suggesting that these genes are the potential origins of significant genetic networks. The distinct sets of differentially expressed genes within each group provide the basis for identifying molecular networks that may aid in understanding the complex interactions between stress and ethanol, and potentially provide relevant therapeutic targets. Using Ptp4a1, a candidate gene underlying the quantitative trait locus for several of these phenotypes, and network analyses, we show that a large group of differentially expressed genes in the NOE group are highly interrelated, some of which have previously been linked to alcohol addiction or alcohol-related phenotypes.

show abstract

“…These treatments were used to mimic stress effects and several studies reported the effects of the acute and chronic stresses modified differently the hippocampus transcriptome (Li et al, 2013; Stankiewicz et al, 2015). Furthermore, the nature of the stressor is also crucial (Porter et al, 2012; Li et al, 2013; Suri et al, 2014; Stankiewicz et al, 2015). Similarly to stress, modifications of living conditions, as well as long-term mild or intense exercise, can modulate the hippocampus transcriptome (Inoue et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Changes in C57BL6 Mouse Hippocampal Transcriptome Induced by Hypergravity Mimic Acute Corticosterone-Induced Stress

Pulga¹,

Porte²,

Morel³

2016

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Centrifugation is a widely used procedure to study the impact of altered gravity on Earth, as observed during spaceflights, allowing us to understand how a long-term physical constraint can condition the mammalian physiology. It is known that mice, placed in classical cages and maintained during 21 days in a centrifuge at 3G gravity level, undergo physiological adaptations due to hypergravity, and/or stress. Indeed, an increase of corticosterone levels has been previously measured in the plasma of 3G-exposed mice. Corticosterone is known to modify neuronal activity during memory processes. Although learning and memory performances cannot be assessed during the centrifugation, literature largely described a large panel of proteins (channels, second messengers, transcription factors, structural proteins) which expressions are modified during memory processing. Thus, we used the Illumina technology to compare the whole hippocampal transcriptome of three groups of C57Bl6/J mice, in order to gain insights into the effects of hypergravity on cerebral functions. Namely, a group of 21 days 3G-centrifuged mice was compared to (1) a group subjected to an acute corticosterone injection, (2) a group receiving a transdermal chronic administration of corticosterone during 21 days, and (3) aged mice because aging could be characterized by a decrease of hippocampus functions and memory impairment. Our results suggest that hypergravity stress induced by corticosterone administration and aging modulate the expression of genes in the hippocampus. However, the modulations of the transcriptome observed in these conditions are not identical. Hypergravity affects per-se the hippocampus transcriptome and probably modifies its activity. Hypergravity induced changes in hippocampal transcriptome were more similar to acute injection than chronic diffusion of corticosterone or aging.

show abstract

Gene Expression Profile of the Hippocampus of Rats Subjected to Chronic Immobilization Stress

Cited by 33 publications

References 83 publications

Effects of the Chinese Traditional Prescription Xiaoyaosan Decoction on Chronic Immobilization Stress-Induced Changes in Behavior and Ultrastructure in Rat Hippocampus

Effects of the Chinese Traditional Prescription Xiaoyaosan Decoction on Chronic Immobilization Stress-Induced Changes in Behavior and Ultrastructure in Rat Hippocampus

Analyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice

Changes in C57BL6 Mouse Hippocampal Transcriptome Induced by Hypergravity Mimic Acute Corticosterone-Induced Stress

Contact Info

Product

Resources

About