Gene expression profile of leiomyoma and myometrium and the effect of gonadotropin releasing hormone analogue therapy

Abstract

Microarray analysis allowed us to identify the profile expression of several specific genes in leiomyoma and myometrium whose expression appears to be differentially regulated after GnRHa therapy.

Expression profile of several genes in human myometrium and uterine leiomyoma

“…This screening approach enabled us to categorize several genes into specific functional groups. Our results corroborate other studies involving cDNA arrays or are at variance with published results (5,6,9).…”

“…Recent reports have cited the overexpression of enzymes, cell-growth regulators, insulin-like growth factor-1, and high-mobility group architectural (HMG) proteins in leiomyomas compared with in myometrium (5)(6)(7)(8)(9)(10). Underexpression of purported tumor suppressors and protooncogenes also is reported (5,6,11).…”

Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in levonorgestrel treated uterine leiomyoma cells

“…Genomic instability of these identified genes, involving estrogen and progesterone receptors were suspected to be associated with an increased risk of leiomyoma development, however the evidence has been inconsistent in a large scale of cases [9][10][11]. Chegini et al and Boryana et al have afforded further insights into the molecular mechanisms of leiomyoma initiation and development by highthroughput sequencing technology [12,13], demonstrating that Background/aims: Uterine leiomyoma is the ahead benign tumor of the female genital tract, which resulted in menstrual abnormalities, recurrent pregnancy loss, and other serious gynecological disorders in women. Recently, as the process of exploring the brief molecular mechanisms of tumorgenesis, microRNAs (miRNAs) have attracted much more attention.…”

Identification of differential proteins in uterine leiomyoma by two-dimensional electrophoresis

“…The molecular causes of the disease remain unknown [1][2][3][4][5][11][12][13][14] . A variety of techniques, including differential hybridization, differential display PCR, representational difference analysis, suppression subtractive hybridization (SSH), and gene array, have been used to identify the genes whose expression is selectively altered in uterine leiomyoma [1][2][3][12][13][14] . All these methods are used to analyze the mRNA expression levels of uterine leiomyoma relative genes.…”

“…Uterine leiomyomas are the most common tumor in the female genital tract, but their etiology and the molecular basis of their development and growth are unclear [1][2][3][4][5] . So it is very important to explore the molecular mechanism of the cause of uterine leiomyoma at protein level by studying the differential proteins between uterine leiomyoma and normal myometrium.…”