Gene expression profile of bladder tissue of patients with ulcerative interstitial cystitis

Gamper, Marianne; Viereck, Volker; Geissbühler, Verena; Eberhard, Jakob; Binder, Jochen; Moll, Carlo; Rehrauer, Hubert; Moser, René

doi:10.1186/1471-2164-10-199

Cited by 60 publications

(66 citation statements)

References 55 publications

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“…7 Biopsies from visibly normal bladder areas in patients with HL have shown inflammation on conventional histology 7 and inflammatory gene expression on microarray analysis. 24,25 Thus, some patients without HL may have ongoing bladder inflammation that responds to CyA. Another explanation is that CyA has several effects that may benefit IC/BPS in addition to its anti-inflammatory properties.…”

Section: Discussionmentioning

confidence: 97%

Cyclosporine A for Refractory Interstitial Cystitis/Bladder Pain Syndrome: Experience of 3 Tertiary Centers

2012

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Cyclosporine A had a high success rate for patients with Hunner lesions in whom more conservative options, including endoscopic treatment, had failed. The success rate was low for patients without Hunner lesions. A 3 to 4-month trial is sufficient time to assess response. Adverse events were common and led to discontinuation of cyclosporine A for some patients. Close monitoring is needed, especially for blood pressure and renal function.

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Section: Discussionmentioning

confidence: 97%

Cyclosporine A for Refractory Interstitial Cystitis/Bladder Pain Syndrome: Experience of 3 Tertiary Centers

2012

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“…Repeat attempts to obtain valid biopsy samples by invasive surgery using general anesthesia are not feasible, costeffective or necessary since extrapolation of the mast cell count to 1 mm 2 yielded similar results. With respect to differentiating BPS/IC with or without Hunner lesions from OAB we propose lymphocyte infiltration 15,16 and urothelium integrity 16 as superior histopathological criteria.…”

Section: Discussionmentioning

confidence: 99%

Are Mast Cells Still Good Biomarkers for Bladder Pain Syndrome/Interstitial Cystitis?

et al. 2015

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“…Recently, Gamper et al . [14] reported a quantitative transcriptomic analysis of bladder biopsies containing mucosa, lamina propria and detrusor muscle cells (at varying relative ratios and biopsy depth) from five patients with ulcerative IC and six healthy individuals as controls using Affymetrix GeneChip expression arrays (Affymetrix Corp., Santa Clara, CA, USA), with over 54 000 probe sets on the chip [14]. The study by Gamper et al .…”

Section: Introductionmentioning

confidence: 99%

“…The study by Gamper et al . [14] is the largest published in vivo evaluation of tissues from human IC subjects without intervening cell culture steps. In an attempt to identify the potential proteins and genes regulated by APF in vivo , and to possibly expand the APF-regulated network identified by SILAC, we performed an integration analysis of our own SILAC data and the microarray data of Gamper et al .…”

Section: Introductionmentioning

confidence: 99%

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Integration analysis of quantitative proteomics and transcriptomics data identifies potential targets of frizzled‐8 protein‐related antiproliferative factor in vivo

Yang

Kim

et al. 2012

BJU International

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Objectives To enhance our understanding of the interstitial cystitis urine biomarker antiproliferative factor (APF), as well as interstitial cystitis biology more generally at the systems level, we reanalyzed recently published large-scale quantitative proteomics and in vivo transcriptomics data sets using an integration analysis tool that we have developed. Materials and methods To identify more differentially expressed genes with a lower false discovery rate from a previously published microarray data set, an integrative hypothesis-testing statistical approach was applied. For validation experiments, expression and phosphorylation levels of select proteins were evaluated by western blotting. Results Integration analysis of this transcriptomics data set with our own quantitative proteomics data set identified 10 genes that are potentially regulated by APF in vivo from 4140 differentially expressed genes identified with a false discovery rate of 1%. Of these, five (i.e. JUP, MAPKSP1, GSPT1, PTGS2/COX-2 and XPOT) were found to be prominent after network modelling of the common genes identified in the proteomics and microarray studies. This molecular signature reflects the biological processes of cell adhesion, cell proliferation and inflammation, which is consistent with the known physiological effects of APF. Lastly, we found the mammalian target of rapamycin pathway was down-regulated in response to APF. Conclusions This unbiased integration analysis of in vitro quantitative proteomics data with in vivo quantitative transcriptomics data led to the identification of potential downstream mediators of the APF signal transduction pathway.

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Gene expression profile of bladder tissue of patients with ulcerative interstitial cystitis

Cited by 60 publications

References 55 publications

Cyclosporine A for Refractory Interstitial Cystitis/Bladder Pain Syndrome: Experience of 3 Tertiary Centers

Cyclosporine A for Refractory Interstitial Cystitis/Bladder Pain Syndrome: Experience of 3 Tertiary Centers

Are Mast Cells Still Good Biomarkers for Bladder Pain Syndrome/Interstitial Cystitis?

Integration analysis of quantitative proteomics and transcriptomics data identifies potential targets of frizzled‐8 protein‐related antiproliferative factor in vivo

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