Gene expression profile evaluation of integrins in 3T3-L1 cells differentiated to adipocyte, insulin resistant and hypertrophied cells

Malekpour-Dehkordi, Zahra; Mohiti-Ardakani, Javad; Nourbakhsh, Mitra; Teimourian, Shahram; Naghiaee, Yousof; Hemati, Mahdie; Jafary, Fariba

doi:10.1016/j.gene.2019.06.019

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…At later stages, our results demonstrates that differentiated beige adipocytes present lower levels of ITA7. In contrast, further upregulation of ITA7 have been seen in insulin resistant and hypertrophied 3T3-l1 adipocytes 38 . Recent studies have shown that brown/beige adipocytes can also be derived from muscle progenitor cells.…”

Section: Discussionmentioning

confidence: 92%

“…ITB1 is widely present in many cells, playing a role in a broad range of cellular processes, making ITB1 a difficult target for therapeutic purposes. ITA7 has been linked with the regulation of cell adhesion and migration 37 , and although its role in adipocytes has not been studied, ITA7 is strongly upregulated in differentiated white adipocytes compared to ASC 38,39 . Our results showed that ITA7 levels in hASCs are also upregulated in the initial stages of beige adipocyte differentiation, but at later stages it decreases as UCP1 levels increase.…”

Section: Discussionmentioning

confidence: 99%

“…The role of the ITA7 spatial distribution in beige adipogenesis remains to be studied. More ITA7 positive cells could indicate an increase in ASC differentiation to white adipocytes, since ITA7 increases during the process of white adipocyte differentiation 38,39 . Less hyperplasia (increased in adipocyte number) in SubQ of KO mice could explain their resistant to obesity and enhanced metabolic function that was previously observed 18 .…”

Section: Discussionmentioning

confidence: 99%

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Integrins and extracellular matrix proteins modulate adipocyte thermogenic capacity

Porras

Stojkova

Vaicik

et al. 2021

Sci Rep

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Obesity and the metabolic disease epidemic has led to an increase in morbidity and mortality. A rise in adipose thermogenic capacity via activation of brown or beige fat is a potential treatment for metabolic diseases. However, an understanding of how local factors control adipocyte fate is limited. Mice with a null mutation in the laminin α4 (LAMA4) gene (KO) exhibit resistance to obesity and enhanced expression of thermogenic fat markers in white adipose tissue (WAT). In this study, changes in WAT extracellular matrix composition in the absence of LAMA4 were evaluated using liquid chromatography/tandem mass spectrometry. KO-mice showed lower levels of collagen 1A1 and 3A1, and integrins α7 (ITA7) and β1 (ITB1). ITA7-ITB1 and collagen 1A1-3A1 protein levels were lower in brown adipose tissue compared to WAT in wild-type mice. Immunohistochemical staining confirmed lower levels and different spatial distribution of ITA7 in KO-WAT. In culture studies, ITA7 and LAMA4 levels decreased following a 12-day differentiation of adipose-derived stem cells into beige fat, and knock-down of ITA7 during differentiation increased beiging. These results demonstrate that extracellular matrix interactions regulate adipocyte thermogenic capacity and that ITA7 plays a role in beige adipose formation. A better understanding of the mechanisms underlying these interactions can be used to improve systemic energy metabolism and glucose homeostasis.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Integrins and extracellular matrix proteins modulate adipocyte thermogenic capacity

Porras

Stojkova

Vaicik

et al. 2021

Sci Rep

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“…The ERK pathway, on the other hand, also plays a vital role in pathological adipogenesis [33]. The obesityrelated hypertrophic adipocytes might express metabolic dysfunction through different biochemical properties, including pro-inflammatory cytokine secretion, hypoxia, elevated lipolysis, and fibrosis [28,34].…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of TG‐interacting factor 1 at carboxyl‐terminal sites in response to insulin regulates adipocyte differentiation

Chang,

Tseng,

Wang

et al. 2024

FEBS Letters

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TG‐interacting factor 1 (TGIF1) contributes to the differentiation of murine white preadipocyte and human adipose tissue‐derived stem cells; however, its regulation is not well elucidated. Insulin is a component of the adipogenic cocktail that induces ERK signaling. TGIF1 phosphorylation and sustained stability in response to insulin were reduced through the use of specific MEK inhibitor U0126. Mutagenesis at T235 or T239 residue of TGIF1 in preadipocytes led to dephosphorylation of TGIF1. The reduced TGIF1 stability resulted in an increase in p27kip1 expression, a decrease in phosphorylated Rb expression and cellular proliferation, and a reduced accumulation of lipids compared to the TGIF1‐overexpressed cells. These findings highlight that insulin/ERK‐driven phosphorylation of the T235 or T239 residue at TGIF1 is crucial for adipocyte differentiation.

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“…All four MAPKs were associated with adipocyte differentiation and lipid metabolism. ERK1/2, the first member of the MAPK family, reportedly, participates in adipocyte differentiation regulation (Malekpour‐Dehkordi et al, 2019). The p38‐MAPK is a major kinase in the MAPK family, and is strongly related to pre‐adipocyte differentiation (Engelman et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

RNA sequencing analysis of the longissimus dorsi to identify candidate genes underlying the intramuscular fat content in Anqing Six‐end‐white pigs

et al. 2023

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Intramuscular fat (IMF) is a significant marker for pork quality. The Anqing Six‐end‐white pig has the characteristics of high meat quality and IMF content. Owing to the influence of European commercial pigs and a late start in resource conservation, the IMF content within local populations varies between individuals. This study analyzed the longissimus dorsi transcriptome of purebred Anqing Six‐end‐white pigs with varying IMF content to recognize differentially expressed genes. We identified 1528 differentially expressed genes between the pigs with high (H) and low (L) IMF content. Based on these data, 1775 Gene Ontology terms were significantly enriched, including lipid metabolism, modification and storage, and regulation of lipid biosynthesis. Pathway analysis revealed 79 significantly enriched pathways, including the Peroxisome proliferator‐activated receptor and mitogen‐activated protein kinase signaling pathways. Moreover, gene set enrichment analysis indicated that the L group had increased the expression of genes related to ribosome function. Additionally, the protein–protein interaction network analyses revealed that VEGFA, KDR, LEP, IRS1, IGF1R, FLT1 and FLT4 were promising candidate genes associated with the IMF content. Our study identified the candidate genes and pathways involved in IMF deposition and lipid metabolism and provides data for developing local pig germplasm resources.

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Gene expression profile evaluation of integrins in 3T3-L1 cells differentiated to adipocyte, insulin resistant and hypertrophied cells

Cited by 7 publications

References 33 publications

Integrins and extracellular matrix proteins modulate adipocyte thermogenic capacity

Integrins and extracellular matrix proteins modulate adipocyte thermogenic capacity

Phosphorylation of TG‐interacting factor 1 at carboxyl‐terminal sites in response to insulin regulates adipocyte differentiation

RNA sequencing analysis of the longissimus dorsi to identify candidate genes underlying the intramuscular fat content in Anqing Six‐end‐white pigs

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