Gene expression profile changes induced by acute toxicity of benzo[a]pyrene in marine medaka

Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 2 – Proteomics and metabolomics

“…Hemopexin is a plasma protein with a very high binding affinity for heme complexes (cytochromes, iron and porphyrin complexes), and it plays a protective role against heme-induced oxidative stress (Ingoglia et al, 2017;Tolosano and Altruda, 2002). Altered gene expression or protein levels for hemopexin were already reported in several studies involving different fish species exposed to benzo[a]pyrene or complex PAHs mixtures (Alderman et al, 2017;Bohne-Kjersem et al, 2009;Enerstvedt et al, 2017;Karlsen et al, 2011;Won et al, 2013). Interestingly, pyrene was also able to increase the expression of several proteins involved in porphyrin metabolism (Supplementary file 4B), notably uroporphyrinogen decarboxylase (UROD) which was also upregulated at the transcriptomic level after 7 days of exposure in our related study (submitted manuscript).…”

Gene expression profile changes induced by acute toxicity of [C16mim]Cl in loach (Paramisgurnus dabryanus)

“…). Wap65, C3a, and CF1 all function in a multi‐protein complex in the immune response (Won et al, ). Thus, it is likely that the immune system of the loach was severely damaged by [C 16 mim]Cl exposure.…”

“…At present, there are three major types of toxic metabolic enzymes, including cytochrome P450s (CYPs), glutathione S ‐transferases (GSTs), and acetyl transferases. Among them, Cyp1a metabolizes many xenobiotic compounds, pesticides, and petroleum products (Won et al, ). Induction of cyp1a mRNA by organic pollutants has been widely reported in various fish species (Williams et al, ; Fisher et al, ; Won et al, ).…”

“…Among them, Cyp1a metabolizes many xenobiotic compounds, pesticides, and petroleum products (Won et al, ). Induction of cyp1a mRNA by organic pollutants has been widely reported in various fish species (Williams et al, ; Fisher et al, ; Won et al, ). Additionally, repression of cyp1a1 and cyp1a2 transcription due to oxidative stress has also been observed in hepatocytes (Barker et al, ).…”

“…Wap65 has two isomers, Wap65-1 and Wap65-2, which are homologous with rat hemopexin (Kikuchi et al, 1995;Won et al, 2013). Previous studies showed that the hemopexinlike protein or Wap65 homolog mRNA level increases significantly in fish under stress, such as hypoxia, pathogen challenge, or warm temperature acclimation (Kikuchi et al, 1995;Gracey et al, 2001).…”