Gene Expression Patterns in Renal Cell Carcinoma Assessed by Complementary DNA Microarray

Higgins, Julian P T; Shinghal, Rajesh; Gill, Harcharan; Reese, Jeffrey; Terris, Martha K.; Cohen, Ronald J.; Fero, M.J.; Pollack, Jonathan R.; Rijn, Matt van de; Brooks, James D.

doi:10.1016/s0002-9440(10)63887-4

Cited by 239 publications

(187 citation statements)

References 41 publications

(40 reference statements)

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“…Disease-specific survival of RCC patients with type I hCG␤ mRNApositive tumors (n ϭ 42) compared with those with hCG␤ mRNA-negative tumors (n ϭ 62; log-rank P ϭ 0.0234; Breslow P ϭ 0.0029 (A), diseasespecific survival in CRCCs (n ϭ 79; log-rank P ϭ 0.15 and Breslow P ϭ 0.026) (B), and papillary chromophobe as well as unclassified tumors (n ϭ 25; log-rank P ϭ 0.017; Breslow P ϭ 0.019) (C). 8,No. 5 tumors, which generally bear a more favorable prognosis than CRCCs.…”

Section: Discussionmentioning

confidence: 99%

Expression Of Human Chorionic Gonadotropin β-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma

Hotakainen

Lintula

Ljungberg

et al. 2006

The Journal of Molecular Diagnostics

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Expression of the free ␤-subunit of human chorionic gonadotropin (hCG␤) in malignant tumors is frequently associated with aggressive disease. The pretreatment serum concentration of hCG␤ is an independent prognostic variable in renal cell carcinoma (RCC). The three so-called type II genes (hCG␤ 3/9, 5, and 8) have been shown to be up-regulated in relation to type I genes (hCG␤ 6/7) in some malignant tumors. We developed a reverse transcription-polymerase chain reaction method for quantification of relative levels of the mRNAs for the two types of hCG␤ genes and studied the association between the expression in RCC tissue (n ‫؍‬ 104) and clinical outcome. hCG␤ mRNA expression was detected in 40% (42 of 104) of the tumors, and in 40 of these (93%), this consisted of hCG␤ type I mRNA only, whereas type II hCG␤ mRNA was detected in two samples. hCG␤ mRNA expression was significantly associated with a shorter diseasespecific (log-rank P ‫؍‬ 0.023; median survival 1.4 versus 7.9 years) and overall survival (log-rank P ‫؍‬ 0.011). In a Cox regression model, stage (P < 0.0001) and hCG␤ mRNA expression (P < 0.0001) were independent prognostic variables. We conclude that expression of type I hCG␤ genes indicates adverse prognosis in

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Section: Discussionmentioning

confidence: 99%

Expression Of Human Chorionic Gonadotropin β-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma

Hotakainen

Lintula

Ljungberg

et al. 2006

The Journal of Molecular Diagnostics

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“…[1][2][3] Patients diagnosed with metastatic disease have a very poor prognosis because these tumors are commonly resistant to chemotherapy and radiotherapy. Immunotherapeutic treatment options are associated with very high toxicities and poor outcomes.…”

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“…Immunotherapeutic treatment options are associated with very high toxicities and poor outcomes. 1,2,4 The current classification system for RCC has a number of subtypes. 5 The two most common subtypes are conventional or clear cell RCC (ccRCC), representing approximately 70 -80% of patients, and papillary RCC (PRCC), which is reportedly observed in only 10 -15% of patients.…”

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confidence: 99%

“…5 The two most common subtypes are conventional or clear cell RCC (ccRCC), representing approximately 70 -80% of patients, and papillary RCC (PRCC), which is reportedly observed in only 10 -15% of patients. 2,3,5 A number of distinguishing features at the macroscopic, microscopic, and cytogenetic levels have been recognized that suggest these tumors differ significantly. These include the hypervascular nature of ccRCC compared with the characteristically hypovascular appearance of PRCC, the presence of infiltrating lymphocytes and macrophages in PRCC but not in ccRCC, and the increased incidence of necrosis in patients with PRCC but not in patients with ccRCC.…”

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“…Distinct karyotypic aberrations also set these subtypes apart, such as loss of 3p in ccRCC compared with trisomies of 7,12,16,17, and 20 and loss of the Y chromosome in PRCC. 2,5,6 The endothelin axis comprises three endothelin peptides (ET-1, ET-2, and ET-3) and two cell-surface receptors (ET A and ET B ). Preproforms of each endothelin (PPET-1, PPET-2, and PPET-3) are cleaved to form propeptides, referred to as big ETs, which are further processed by endothelin-converting enzymes (ECEs) to generate the active 21 amino acid peptide ligands for the two receptors.…”

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Endothelin axis expression is markedly different in the two main subtypes of renal cell carcinoma

Douglas

Richardson

Nicol

2004

Cancer

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BACKGROUNDThe endothelin axis has been implicated in cancer growth, angiogenesis, and metastasis, but to the authors' knowledge the expression of endothelin genes has not been defined in renal cell carcinoma (RCC).METHODSTissue specimens were harvested from both normal and tumor‐affected regions at the time of radical nephrectomy from 35 patients with RCC (22 with clear cell RCC [ccRCC] and 13 with papillary RCC [PRCC]). Real‐time reverse transcriptase‐polymerase chain reaction analysis determined the expression profile of the preproendothelins (PPET‐1, PPET‐2, and PPET‐3), the endothelin receptors (ETA and ETB), and the endothelin‐converting enzymes (ECE‐1 and ECE‐2).RESULTSPPET‐1 was found to be up‐regulated in ccRCC tumor specimens and down‐regulated in PRCC tumor specimens. ETA was significantly down‐regulated in PRCC tumor specimens. ECE‐1 was expressed in all tissue specimens at comparable levels, with moderate but significant elevation in normal tissue specimens associated with PRCC. Of the other genes, PPET‐2 and ETB were expressed in all tissue specimens and no differences were observed between tumor subtypes or tumor‐affected and normal tissue specimens, whereas PPET‐3 and ECE‐2 were present in all tissue specimens but were barely detectable.CONCLUSIONSThe endothelin axis was expressed differently in the two main subtypes of RCC and appeared to match macroscopic features commonly observed in these tumors (i.e., high expression of PPET‐1 in hypervascular ccRCC contrasted against low PPET‐1 and ETA expression in hypovascular PRCC). The presence of ECE‐1 mRNA in these tissue specimens suggested that active endothelin ligands were present, indicating endothelin axis activity was elevated in ccRCC compared with normal kidney, but impaired in PRCC. The current study provided further evidence that it is not appropriate to consider ccRCC and PRCC indiscriminately in regard to treatment. Cancer 2004. © 2004 American Cancer Society.

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Current awareness on comparative and functional genomics

2003

Comp Funct Genom

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on comparative and functional genomics. Each bibliography is divided into 16 sections. 1 Reviews & symposia; 2 General; 3 Large‐scale sequencing and mapping; 4 Genome evolution; 5 Comparative genomics; 6 Gene families and regulons; 7 Pharmacogenomics; 8 Large‐scale mutagenesis programmes; 9 Functional complementation; 10 Transcriptomics; 11 Proteomics; 12 Protein structural genomics; 13 Metabolomics; 14 Genomic approaches to development; 15 Technological advances; 16 Bioinformatics. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted

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Gene Expression Patterns in Renal Cell Carcinoma Assessed by Complementary DNA Microarray

Abstract: Renal cell carcinoma comprises 3% of malignancies worldwide and is increasing in incidence.

Cited by 239 publications

References 41 publications

Expression Of Human Chorionic Gonadotropin β-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma

Expression Of Human Chorionic Gonadotropin β-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma

Endothelin axis expression is markedly different in the two main subtypes of renal cell carcinoma

Current awareness on comparative and functional genomics

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