Gene expression patterns during adaptation of a helminth parasite to different environmental niches

Jolly, Emmitt R.; Chin, Chen-Shan; Miller, Steve; Bahgat, Mahmoud Mohamed; Lim, K. C.; DeRisi, Joseph L.; McKerrow, James H.

doi:10.1186/gb-2007-8-4-r65

Cited by 81 publications

(98 citation statements)

References 76 publications

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“…A description of affected genes for each stage is given below. In general, all observed patterns of expression of protein-coding genes among life cycle stages that will be described here, are not inconsistent with the previously published microarray results (Dillon et al 2006, Jolly et al 2007, Fitzpatrick et al 2009, Gobert et al 2009). Here we would like to especially highlight the non-coding genes that were never the focus of study before.…”

Section: Non-coding Expression Signature Among Life Cycle Stagessupporting

confidence: 70%

“…A number of papers already describe differences in gene expression among the developmental stages of schistosomes (Dillon et al 2006, Vermeire et al 2006, Jolly et al 2007, Fitzpatrick et al 2009, Gobert et al 2009). Here we performed a set of experiments with 5 developmental stages (eggs, miracidia, cercariae, 7-dayold schistosomula and adult worms) using a 4k-element cDNA microarray platform that was designed to have a considerable fraction of probes for non-protein-coding genes (1133 probes); a detailed description of this platform is deposited in GEO under accession number GP-L3929 (Demarco et al 2006).…”

Section: Non-coding Expression Signature Among Life Cycle Stagesmentioning

confidence: 99%

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Non-coding RNAs in schistosomes: an unexplored world

Oliveira

Carvalho

Maracaja-Coutinho

et al. 2011

An. Acad. Bras. Ciênc.

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Non-coding RNAs (ncRNAs) were recently given much higher attention due to technical advances in sequencing which expanded the characterization of transcriptomes in different organisms. ncRNAs have different lengths (22 nt to >1,000 nt) and mechanisms of action that essentially comprise a sophisticated gene expression regulation network. Recent publication of schistosome genomes and transcriptomes has increased the description and characterization of a large number of parasite genes. Here we review the number of predicted genes and the coverage of genomic bases in face of the public ESTs dataset available, including a critical appraisal of the evidence and characterization of ncRNAs in schistosomes. We show expression data for ncRNAs in Schistosoma mansoni. We analyze three different microarray experiment datasets: (1) adult worms' large-scale expression measurements; (2) differentially expressed S. mansoni genes regulated by a human cytokine (TNF-α) in a parasite culture; and (3) a stage-specific expression of ncRNAs. All these data point to ncRNAs involved in different biological processes and physiological responses that suggest functionality of these new players in the parasite's biology. Exploring this world is a challenge for the scientists under a new molecular perspective of host-parasite interactions and parasite development.

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Section: Non-coding Expression Signature Among Life Cycle Stagessupporting

confidence: 70%

Section: Non-coding Expression Signature Among Life Cycle Stagesmentioning

confidence: 99%

Non-coding RNAs in schistosomes: an unexplored world

Oliveira

Carvalho

Maracaja-Coutinho

et al. 2011

An. Acad. Bras. Ciênc.

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“…Third, RNAi pathway sharing common components with miRNA is positive for some protozoan parasites (Lemos and Menezes 1978;Blackman 2003). Finally, the complex life cycles of many protozoan parasites require the ability to respond to environmental and developmental signals through regulating gene expression (Jolly et al 2007).…”

Section: Mirnas In Protozoan Parasitesmentioning

confidence: 99%

MicroRNAs of parasites: current status and future perspectives

Liu¹,

Tuo

Gao³

et al. 2010

Parasitol Res

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MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs regulating gene expression in eukaryotes at the post-transcriptional level. The complex life cycles of parasites may require the ability to respond to environmental and developmental signals through miRNA-mediated gene expression. Over the past 17 years, thousands of miRNAs have been identified in the nematode Caenorhabditis elegans and other parasites. Here, we review the current status and potential functions of miRNAs in protozoan, helminths, and arthropods, and propose some perspectives for future studies.

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“…Prior to the sequencing of the genomes, comprehensive transcriptomic datasets (Verjovski-Almeida et al, 2003) and genetic maps (Criscione et al, 2009) were published, providing scaffolds with which to assemble the genome sequences. DNA microarrays have been generated by multiple groups and utilized to explore transcriptional profiles of different developmental stages of the parasite (Fitzpatrick et al, 2009;Gobert et al, 2009bGobert et al, , 2010Jolly et al, 2007) (Fig. 1) and the transcriptional effects of different treatments (Gobert, 2010;Gobert et al, 2010).…”

Section: Schistosomesmentioning

confidence: 99%

Vaccinomics for the Major Blood Feeding Helminths of Humans

Loukas

Gaze

Mulvenna

et al. 2011

OMICS: A Journal of Integrative Biology

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Approximately one billion people are infected with hookworms and/or blood flukes (schistosomes) in developing countries. These two parasites are responsible for more disability adjusted life years lost than most other neglected tropical diseases (NTDs), and together, are second only to malaria. Although anthelmintic drugs are effective and widely available, they do not protect against reinfection, resistant parasites are likely to emerge, and mass drug administration programs are unsustainable. Therefore, there is a pressing need for the development of vaccines against these parasites. In recent years, there have been major advances in our understanding of hookworms and schistosomes at the molecular level through the use of ''omics'' technologies. The secretomes of these parasites have been characterized using transcriptomics, genomics, proteomics, and newly developed gene manipulation and silencing techniques, and the proteins of interest are now the target of novel antigen discovery approaches, notably immunomics. This research has resulted in the discovery, development, and early stage clinical trials of subunit vaccines against hookworms and schistosomes.

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Gene expression patterns during adaptation of a helminth parasite to different environmental niches

Abstract: Using a genomic microarray, gene expression at three different developmental stages of the schistosome parasite were analyzed, resulting in the identification of 1154 developmentally enriched transcripts.

Cited by 81 publications

References 76 publications

Non-coding RNAs in schistosomes: an unexplored world

Non-coding RNAs in schistosomes: an unexplored world

MicroRNAs of parasites: current status and future perspectives

Vaccinomics for the Major Blood Feeding Helminths of Humans

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