Gene expression patterns associated with chicken jejunal development

Schokker, Dirkjan; Hoekman, A.J.W.; Smits, M.A.; Rebel, Johanna M.J.

doi:10.1016/j.dci.2009.06.002

Cited by 34 publications

(31 citation statements)

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“…Briefly, total RNA was extracted from 50 to 100 mg jejunum tissue. Jejunum is the intestinal segment of interest, mainly because the jejunum is both involved in absorption of nutrients and minerals, exerts immunological activity and in early life this segment is involved in the programming of the (local) immune system [13, 14]. Each sample was handled individually and subsequently pools were made for further analyses.…”

Section: Methodsmentioning

confidence: 99%

“…Dietary interventions at young age, such as the usage of (pre)starter feeds, prebiotics, probiotics and antibiotics, are regarded to affect the crosstalk between microbiota and host mucosal cells in the intestinal tract, which may result in a change of immune development [8–11]. During the first weeks of life different categories of immunological processes have been identified in broilers [12–14]. Based on spatio-temporal gene expression profiles, the following sequential order for immune related processes have been reported: 1) innate development and influx of immune cells; 2) immune differentiation and specialization; and 3) maturation and immune regulation [14].…”

Section: Introductionmentioning

confidence: 99%

“…During the first weeks of life different categories of immunological processes have been identified in broilers [12–14]. Based on spatio-temporal gene expression profiles, the following sequential order for immune related processes have been reported: 1) innate development and influx of immune cells; 2) immune differentiation and specialization; and 3) maturation and immune regulation [14]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Schokker

Jansman

Veninga³

et al. 2017

BMC Genomics

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BackgroundGut microbial colonization and development of immune competence are intertwined and are influenced by early-life nutritional, environmental, and management factors. Perturbation of the gut microbiome at young age affects the crosstalk between intestinal bacteria and host cells of the intestinal mucosa.ResultsWe investigated the effect of a perturbation of the normal early life microbial colonization of the jejunum in 1-day old chickens. Perturbation was induced by administering 0.8 mg amoxicillin per bird per day) via the drinking water for a period of 24 h. Effects of the perturbation were measured by 16S rRNA profiling of the microbiome and whole genome gene expression analysis. In parallel to what has been observed for other animal species, we hypothesized that such an intervention may have negative impact on immune development.Trends were observed in changes of the composition and diversity of the microbiome when comparing antibiotic treated birds with their controls. in the jejunum, the expression of numerous genes changed, which potentially leads to changes in biological activities of the small intestinal mucosa. Validation of the predicted functional changes was performed by staining immune cells in the small intestinal mucosa and a reduction in the number of macrophage-like (KUL01+) cells was observed due to a direct or indirect effect of the antibiotic treatment. We provide evidence that a short, early life antibiotic treatment affects both the intestinal microbiota (temporarily) and mucosal gene expression over a period of 2 weeks.ConclusionThese results underscore the importance of early life microbial colonization of the gut in relation to immune development and the necessity to explore the capabilities of a variety of early life dietary and/or environmental factors to modulate the programming for immune competence in broilers.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3625-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Schokker

Jansman

Veninga³

et al. 2017

BMC Genomics

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“…These become functional during the first 2 weeks of post-hatch development (Bar-Shira et al, 2003). Shifts in gene expression including those associated with the innate immune functioning have been reported during development of the jejunum of the chicken (Schokker et al, 2009). There are marked shifts in immunoglobulin and cytokine expression in the small intestine during chicken Based on Coates et al, 1968. post hatch development with peak expression of IgM during week 1, of IgY in week 5 and IgA after week 7, and of IL-1, IL-10, IL-12p40, iNOS and interferon-γ between days 14 and 42 (Lammers et al, 2010).…”

Section: Development Of the Gastro-intestinal Tractmentioning

confidence: 98%

Biology of the Gastrointestinal Tract in Poultry

Scanes

Pierzchała-Koziec

2014

Avian Biology Research

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This review provides an overview of the anatomy and physiology of the gastro-intestinal tract (GIT) of poultry, particularly of the domesticated chicken. The structure and functioning of the major regions of the GIT are discussed bringing together recent studies with the older, often neglected, literature. Attention is focused on the GIT as an immune organ and on GIT fermentation/bacterial colonisation. In addition, the interactions of nutrition with GIT biology are discussed. The roles of neuropeptides and hormones on the development and functioning of the GIT are extensively reviewed.

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“…In the present study, greater PepT1 expression than SGLT1 probably related to the importance of proteins during development and may be necessary to maximize amino acid assimilation when the feed become available (Mott et al, 2008). It was previously described that genes that are important for functional developments should have the highest expression levels at early life (Schokker et al, 2009). On the other hand, it was also reported an increase in PepT1 expression in response to starvation in rats (Ihara et al, 2000) and chickens (Mott et al, 2008).…”

Section: Discussionmentioning

confidence: 50%

Egg storage duration and hatch window affect gene expression of nutrient transporters and intestine morphological parameters of early hatched broiler chicks

Yalçın

Gürsel

Bilgen

et al. 2016

Animal

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In recent years, researchers have given emphasis on the differences in physiological parameters between early and late hatched chicks within a hatch window. Considering the importance of intestine development in newly hatched chicks, however, changes in gene expression of nutrient transporters in the jejunum of early hatched chicks within a hatch window have not been studied yet. This study was conducted to determine the effects of egg storage duration before incubation and hatch window on intestinal development and expression of PepT1 (H + -dependent peptide transporter) and SGLT1 (sodium-glucose co-transporter) genes in the jejunum of early hatched broiler chicks within a 30 h of hatch window. A total of 1218 eggs obtained from 38-week-old Ross 308 broiler breeder flocks were stored for 3 (ES3) or 14 days (ES14) and incubated at the same conditions. Eggs were checked between 475 and 480 h of incubation and 40 chicks from each egg storage duration were weighed; chick length and rectal temperature were measured. The chicks were sampled to evaluate morphological parameters and PepT1 and SGLT1 expression. The remaining chicks that hatched between 475 and 480 h were placed back in the incubator and the same measurements were conducted with those chicks at the end of hatch window at 510 h of incubation. Chick length, chick dry matter content, rectal temperature and weight of small intestine segments increased, whereas chick weight decreased during the hatch window. The increase in the jejunum length and villus width and area during the hatch window were higher for ES3 than ES14 chicks. PepT1 expression was higher for ES3 chicks compared with ES14. There was a 10.2 and 17.6-fold increase in PepT1 and SGLT1 expression of ES3 chicks at the end of hatch window, whereas it was only 2.3 and 3.3-fold, respectively, for ES14 chicks. These results suggested that egg storage duration affected development of early hatched chicks during 30 h of hatch window. It can be concluded that the ES14 chicks would be less efficiently adapted to absorption process for carbohydrates and protein than those from ES3 at the end of the hatch window.

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Gene expression patterns associated with chicken jejunal development

Cited by 34 publications

References 84 publications

Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Biology of the Gastrointestinal Tract in Poultry

Egg storage duration and hatch window affect gene expression of nutrient transporters and intestine morphological parameters of early hatched broiler chicks

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