“…Some studies presented a decrease in cleavage and blastocyst rates; however, the embryos were subjected to immediately change of temperature, without adapting period. (Pavani et al, 2016;Sakatani, Yamanaka, Balboula, Takenouchi, & Takahashi, 2015;Sugiyama, McGowan, Kafi, Phillips, & Young, 2003;Yamanaka et al, 2018). Our results have shown that HS during oocyte maturation, fertilization and/or embryo Trophoblastic cells produce IFNT, which is essential for maternal recognition of pregnancy (Spencer, Johnson, Bazer, & Burghardt, 2004), it is also a good marker for embryonic health (Roberts, Chen, Ezashi, & Walker, 2008).…”