Gene Expression of Secretory Carbonic Anhydrase Isozymes in Striated Ducts of Canine Salivary Glands Using Laser Microdissection System

Ichihara, Nobutsune; Tsukamoto, Atsushi; Kasuya, Tamae; Shibata, S.; Nishita, Toshiho; Murakami, Masaru; Amasaki, Hajime; Asari, Masao

doi:10.1111/j.1439-0264.2007.00774.x

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The CAs physically interact with and regulate the activity of several HCO 3 − transporters, including Na + -HCO 3 − co-transporters and the AE and SLC26A6 Cl − /HCO 3 − exchangers (269). Immunolocalization suggested localization of CAII, CAIV, CAIX, and CAXII in pancreatic (301) and multiple isoforms in salivary gland ducts (18, 172). Several of the ductal Ca 2+ signal are cytoplasmic and some are integral membrane proteins with the catalytic domain in the cell surface.…”

Section: Regulatory Proteins Of Epithelial Hco3− Secretionmentioning

confidence: 99%

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Lee

Ohana

Park

et al. 2012

Physiological Reviews

346

455

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Fluid and HCO3−secretion is a vital function of all epithelia and is required for the survival of the tissue. Aberrant fluid and HCO3−secretion is associated with many epithelial diseases, such as cystic fibrosis, pancreatitis, Sjögren's syndrome, and other epithelial inflammatory and autoimmune diseases. Significant progress has been made over the last 20 years in our understanding of epithelial fluid and HCO3−secretion, in particular by secretory glands. Fluid and HCO3−secretion by secretory glands is a two-step process. Acinar cells secrete isotonic fluid in which the major salt is NaCl. Subsequently, the duct modifies the volume and electrolyte composition of the fluid to absorb the Cl−and secrete HCO3−. The relative volume secreted by acinar and duct cells and modification of electrolyte composition of the secreted fluids varies among secretory glands to meet their physiological functions. In the pancreas, acinar cells secrete a small amount of NaCl-rich fluid, while the duct absorbs the Cl−and secretes HCO3−and the bulk of the fluid in the pancreatic juice. Fluid secretion appears to be driven by active HCO3−secretion. In the salivary glands, acinar cells secrete the bulk of the fluid in the saliva that is driven by active Cl−secretion and contains high concentrations of Na+and Cl−. The salivary glands duct absorbs both the Na+and Cl−and secretes K+and HCO3−. In this review, we focus on the molecular mechanism of fluid and HCO3−secretion by the pancreas and salivary glands, to highlight the similarities of the fundamental mechanisms of acinar and duct cell functions, and to point out the differences to meet gland-specific secretions.

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Section: Regulatory Proteins Of Epithelial Hco3− Secretionmentioning

confidence: 99%

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Lee

Ohana

Park

et al. 2012

Physiological Reviews

346

455

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“…Carbonic anhydrase is a type of metalloenzyme that catalyses the conversion of carbon dioxide to bicarbonate and protons, and affects the secretion of saliva and function of digestion in the upper alimentary tract by controlling the acid–base balance and movement of ions and water in digestive organs (Thashian, 1989, 1992). In mammals, four of 14 currently identified CA isozymes, cytosolic CA‐I, II and III and secretory CA‐VI, are mainly found in salivary glands and are thought to play an important role in the process of digestion (Thashian, 1989, 1992; Ichihara et al., 2007). Expression patterns of these isozymes in the salivary glands may provide important information regarding adaptation and differentiation of digestive organs.…”

Section: Introductionmentioning

confidence: 99%

Histological Structure and Distribution of Carbonic Anhydrase Isozymes (CA‐I, II, III and VI) in Major Salivary Glands in Koalas

Mizuno¹,

McKinnon²,

Ichihara³

et al. 2009

Anat Histol Embryol

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While the mandibular glands usually consist of only mucous acinar cells or a combination of mucous and serous cells in other species of mammals, those of koalas were serous glands. Rabbit mono-specific polyclonal anti-canine CA-I, II, III or VI antiserum showed cross-reactivity against corresponding koala carbonic anhydrase (CA) isozymes. Although immunohistochemical reactions to CA-I, II and VI in ductal cells were moderate to strong in the tested salivary glands, no reaction or only slight reactions were observed against CA-III. In the sublingual glands, moderate immunohistochemical reactions to CA-I, II and VI were also evident in serous acinar cells and serous demilunes. However, no reactions to the tested isozymes were observed in mucous acinar cells in these glands. With the exception of the histological structure of the mandibular glands, histological features and the distributional profile of CA isozymes of the salivary glands in koalas are relatively close to results obtained from horses.

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Identification of canine saliva using mRNA-based assay

et al. 2016

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A dog saliva analysis in addition to a bite-mark analysis may be important for evidence when a crime involves a dog bite. In this study, the utility of detecting canine saliva-specific mRNAs to identify canine saliva was evaluated. Canine saliva swabs (n = 20), urine swabs (n = 20), body surface swabs (n = 20), whole blood samples (n = 10), human saliva (n = 20), human skin surface swabs (n = 20), and human whole blood (n = 20) were tested. The saliva-specific genes encoding statherin (STATH), carbonic anhydrase VI (CA-VI), and dog allergens (Canf1 and Canf2) were analyzed as candidate genes. Moreover, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as confirmation of canine mRNA extraction. STATH, CA-VI, Canf1, Canf2, and GAPDH mRNAs were detected in 19/20, 1/20, 11/20, 4/20, and 20/20 saliva samples, respectively. The STATH, CA-VI, Canf1, Canf2, and GAPDH mRNAs did not exhibit cross-reactivity with samples of human origin. This mRNA-based assay was also able to detect canine saliva in mock forensic samples. The results of this study indicated that the detection of STATH mRNA is useful for the identification of canine saliva, and GAPDH is a suitable marker for canine mRNA extraction.

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Gene Expression of Secretory Carbonic Anhydrase Isozymes in Striated Ducts of Canine Salivary Glands Using Laser Microdissection System

Cited by 5 publications

References 19 publications

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Histological Structure and Distribution of Carbonic Anhydrase Isozymes (CA‐I, II, III and VI) in Major Salivary Glands in Koalas

Identification of canine saliva using mRNA-based assay

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Gene Expression of Secretory Carbonic Anhydrase Isozymes in Striated Ducts of Canine Salivary Glands Using Laser Microdissection System

Cited by 5 publications

References 19 publications

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO3−Secretion

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO3−Secretion

Histological Structure and Distribution of Carbonic Anhydrase Isozymes (CA‐I, II, III and VI) in Major Salivary Glands in Koalas

Identification of canine saliva using mRNA-based assay

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Product

Resources

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Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion