Gene expression of PLAT and ATS3 proteins increases plant resistance to insects

Savadogo, Eric Hyrmeya; Shiomi, Yui; Yasuda, Junko; Akino, Toshiharu; Yamaguchi, Masamitsu; Yoshida, Hideki; Umegawachi, Takanari; Tanaka, Ryo; Suong, Dang Ngoc Anh; Miura, Kenji; Yazaki, Kazufumi; Kitajima, Sakihito

doi:10.1007/s00425-020-03530-y

Cited by 6 publications

(3 citation statements)

References 51 publications

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“…With GO enrichment analysis, we found that some proteins were directly involved in regulating the ABA pathway and the seed growth and development pathway, such as AZI1, which regulates the root length of Arabidopsis [ 49 , 50 ], responding to ABA after LSH8 deficiency. Other proteins involved in seed storage, such as LEA family proteins AT5G44310 and LEA (AT2G21490) [ 51 ], CRU2 and CRU3 [ 52 , 53 , 54 , 55 , 56 ], ATS3 [ 51 , 57 ], AT1G03890 [ 52 ] and CRA1 [ 54 , 55 ], could also respond to ABA following LSH8 deficiency. Therefore, it was speculated that LSH8 may also play an important role in the ABA maintenance of seed dormancy.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins

Zou

Tang

et al. 2021

IJMS

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Phytohormone ABA regulates the expression of numerous genes to significantly affect seed dormancy, seed germination and early seedling responses to biotic and abiotic stresses. However, the function of many ABA-responsive genes remains largely unknown. In order to improve the ABA-related signaling network, we conducted a large-scale ABA phenotype screening. LSH, an important transcription factor family, extensively participates in seedling development and floral organogenesis in plants, but whether its family genes are involved in the ABA signaling pathway has not been reported. Here we describe a new function of the transcription factor LSH8 in an ABA signaling pathway. In this study, we found that LSH8 was localized in the nucleus, and the expression level of LSH8 was significantly induced by exogenous ABA at the transcription level and protein level. Meanwhile, seed germination and root length measurements revealed that lsh8 mutant lines were ABA insensitive, whereas LSH8 overexpression lines showed an ABA-hypersensitive phenotype. With further TMT labeling quantitative proteomic analysis, we found that under ABA treatment, ABA-responsive proteins (ARPs) in the lsh8 mutant presented different changing patterns with those in wild-type Col4. Additionally, the number of ARPs contained in the lsh8 mutant was 397, six times the number in wild-type Col4. In addition, qPCR analysis found that under ABA treatment, LSH8 positively mediated the expression of downstream ABA-related genes of ABI3, ABI5, RD29B and RAB18. These results indicate that in Arabidopsis, LSH8 is a novel ABA regulator that could specifically change the expression pattern of APRs to positively mediate ABA responses.

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Section: Discussionmentioning

confidence: 99%

Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins

Zou

Tang

et al. 2021

IJMS

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“…The role of the PLAT domain in function and evolution remains obscure. Recent reports demonstrated that prokaryotic LOX lacking the PLAT domain are fully active [ 1 ], whereas single PLAT proteins exist in eukaryotic organisms such as plants and display similar stress-associated functions as described for LOXs [ 9 ]. In mammals and humans (h), LOX12 and LOX15 a/b are 13-LOX-like enzymes in terms of linolenic acid (LeA) hydroperoxidation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Biochemical Characterization of 13-Lipoxygenases of Arabidopsis thaliana

Maynard

Chibani

Schmidtpott

et al. 2021

IJMS

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13-Lipoxygenases (13-LOX) catalyze the dioxygenation of various polyunsaturated fatty acids (PUFAs), of which α-linolenic acid (LeA) is converted to 13-S-hydroperoxyoctadeca-9, 11, 15-trienoic acid (13-HPOT), the precursor for the prostaglandin-like plant hormones cis-(+)-12-oxophytodienoic acid (12-OPDA) and methyl jasmonate (MJ). This study aimed for characterizing the four annotated A. thaliana 13-LOX enzymes (LOX2, LOX3, LOX4, and LOX6) focusing on synthesis of 12-OPDA and 4Z,7Z,10Z)-12-[[-(1S,5S)-4-oxo-5-(2Z)-pent-2-en-1yl] cyclopent-2-en-1yl] dodeca-4,7,10-trienoic acid (OCPD). In addition, we performed interaction studies of 13-LOXs with ions and molecules to advance our understanding of 13-LOX. Cell imaging indicated plastid targeting of fluorescent proteins fused to 13-LOXs-N-terminal extensions, supporting the prediction of 13-LOX localization to plastids. The apparent maximal velocity (Vmaxapp) values for LOX-catalyzed LeA oxidation were highest for LOX4 (128 nmol.s−1.mg protein−1), with a Km value of 5.8 µM. A. thaliana 13-LOXs, in cascade with 12-OPDA pathway enzymes, synthesized 12-OPDA and OCPD from LeA and docosahexaenoic acid, previously shown only for LOX6. The activities of the four isoforms were differently affected by physiologically relevant chemicals, such as Mg2+, Ca2+, Cu2+ and Cd2+, and by 12-OPDA and MJ. As demonstrated for LOX4, 12-OPDA inhibited enzymatic LeA hydroperoxidation, with half-maximal enzyme inhibition at 48 µM. Biochemical interactions, such as the sensitivity of LOX toward thiol-reactive agents belonging to cyclopentenone prostaglandins, are suggested to occur in human LOX homologs. Furthermore, we conclude that 13-LOXs are isoforms with rather specific functional and regulatory enzymatic features.

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“…Furthermore, Drosophila has advantageous characteristics, including a short life cycle, good reproductive ability, and ease of husbandry. In addition to these properties, it is also compatible with many types of pests, and a highly sensitive experimental system has been established to test the insecticidal activity of plant extracts ( Savadogo et al., 2021 ). Moreover, established libraries of Drosophila mutants and transgenes, as well as transgenic and reporter lines, are available, suggesting that Drosophila offers the opportunity to pursue innovative experimental approaches that are thought to be difficult to implement in other insect model systems ( Pandey and Nichols, 2011 ).…”

Section: Introductionmentioning

confidence: 99%