Gene expression of enzymes involved in utilization of xylooligosaccharides byLactobacillusstrains

Abstract

Prebiotics are defined as food components that confer health benefits on the host through modulation of the microbiota. Xylooligosaccharides (XOS) are non-digestible oligosaccharides that have recently received increasing attention as potential prebiotic candidates. XOS are sugar oligomers composed of 1,4-linked xylopyranosyl backbone and are obtained by either chemical or, more commonly, enzymatic hydrolysis of xylan polysaccharides, extracted from the plant cell wall. The bifidogenic effect of XOS was demonstrated by both in vitro studies and small-scale in vivo human studies. Some intestinal bacterial strains are able to grow on XOS, yet numerous studies have demonstrated that the ability to utilize these oligosaccharides varies considerably among these bacteria. The aim of this study is to investigate the ability of several strains Lactobacillus to use XOS. Fifteen Lactobacillus strains, allifiated to L. plantarum, L. brevis and L. sakei, were studied. Screening procedure was performed for the ability of the strains to utilize XOS as an alternative carbon source. Only some of them utilize XOS. The growth kinetics show the presence of two lag phases, indicating that these bacteria utilize probably some monosaccharides present in the used XOS. XOS were fermented with high specificity by Bifidobacteria strains, but Lactobacilli did not metabolize XOS efficiently.

Metabolic Profiling of Xylooligosaccharides by Lactobacilli

“…brevis —2; Lb. sakei —1) show that the examined strains fermented XOSs with different capacity, changing the optical density [ 23 ]. Our next investigation was focused on the properties of three of the strains: Lb.…”

“…In vitro growth assays and human trials that explored changes in gut microbiota composition after XOS intake also pointed to XOSs as prebiotic polysaccharides that stimulate the growth of certain lactobacilli [ 26 ]. Notwithstanding, the capacity to ferment XOSs by lactobacilli seems to be limited [ 23 , 36 ].…”

Novel Synergistic Mechanism for Lignocellulose Degradation by a Thermophilic Filamentous Fungus and a Thermophilic Actinobacterium Based on Functional Proteomics

“…XOS and xylose were inducers for many microorganisms, for example, T. lanuginosus and Lactobacillus (Maria et al, 2014), while high concentrations of them were inhibitors for T. fusca. The concentration of XOS and xylose, may be a signal factor, could regulate the growth of T. lanuginosus and T. fusca (Figure 7).…”

Combining of transcriptome and metabolome analyses for understanding the utilization and metabolic pathways of Xylo‐oligosaccharide in Bifidobacterium adolescentis ATCC 15703

“…Thus, XOS may enhance the sugar transport process by ABC transporters system. (Kelly et al, 2016;Maria, Margarita, IIlia, & Iskra, 2014). 74↓ ko01220/ko01110/ko00260/ko01130/ko00010/ ko00770/ko00620/ko01100/ko01200/ko00730/ ko01502/ko00622/ko00660/ko00270/ko00760/ ko00710/ko00250/ko00020/ko00440/ko00040/ ko01210/ko00430/ko00030)/ko01230/ko00900/ ko00680/ko00650/ko00630/ko01120/ko00362/ ko00360/ko00621/ko00290/ko00350/ko00053/ ko00473/ko02060/ko00330/ko00261/ko00720…”