Gene expression of cumulus cells in women with poor ovarian response after dehydroepiandrosterone supplementation

Tsui, Kuan‐Hao; Lin, Lie Chwen; Horng, Huann-Cheng; Chang, Renin; Huang, Ben-Shian; Cheng, Jiin‐Tsuey; Wang, Peng-Hui

doi:10.1016/j.tjog.2014.09.003

Cited by 30 publications

(32 citation statements)

References 63 publications

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“…72e74 Thus, investigation of the effect of DHEA on gene expression of CCs may be a good idea to identify the exact mechanisms of DHEA in follicular microenvironment. 45 In our previous study, we found that DHEA supplementation might influence gene expression of CCs in women with POR, and these genes were involved in extracellular matrix formation, cell development, differentiation, and apoptosis regulation. Additionally, changes of the abovementioned genes favored the oocyte maturation.…”

Section: Further Workmentioning

confidence: 92%

“…Additionally, changes of the abovementioned genes favored the oocyte maturation. 45 In conclusion, based on previous research, the effect of DHEA is believed to be mediated by androgen and IGF-1, although the exact mechanisms remain elusive. However, the effect of DHEA on gene changes of CCs may be a promising research field to explore the molecular mechanisms of DHEA on reproduction.…”

Section: Further Workmentioning

confidence: 95%

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Clinical application of dehydroepiandrosterone in reproduction: A review of the evidence

Lin

Tsui

Wang

2015

Journal of the Chinese Medical Association

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The effect of dehydroepiandrosterone (DHEA) therapy on improvement of reproduction outcome is uncertain. Many earlier studies have shown conflicting results. Therefore, a review of the literature is needed to explore the role of DHEA in reproduction. We conducted a literature search of MEDLINE (Ovid) and Pub-Med (2000-June 2014) for all relevant articles that included the terms "dehydroepiandrosterone," "DHEA," and "in vitro fertilization." Among the search-identified articles, seven prospective self-controlled studies and four retrospective case-control studies showed DHEA as an adjuvant therapy able to improve in vitro fertilization outcomes in poor responders (women with diminished ovarian reserve and/or poor ovarian response). However, four randomized controlled trials did not support the benefit of DHEA therapy for poor responders. By contrast, one prospective randomized study showed that DHEA might be beneficial to reproduction in women without diminished ovarian reserve (normal responders). In summary, a review of the previously published studies does not provide clear evidence that DHEA can be a useful treatment to improve ovarian function in poor responders.

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Section: Further Workmentioning

confidence: 92%

Section: Further Workmentioning

confidence: 95%

Clinical application of dehydroepiandrosterone in reproduction: A review of the evidence

Lin

Tsui

Wang

2015

Journal of the Chinese Medical Association

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“…DHEA may improve ovarian function in women with poor ovarian response by activating anti-apoptotic processes in cumulus cells. These processes most likely involve the upregulation of genes related to extracellular matrix (ECM) formation and downregulating genes related to cell development, differentiation, and apoptosis (84). In other areas, DHEA supplementation has been reported to have an effect on regulation of the Th1/Th2 response.…”

Section: Balance Of the Th1 And Th2 Immune Responsementioning

confidence: 99%

Dehydroepiandrosterone improves the ovarian reserve of women with diminished ovarian reserve and is a potential regulator of the immune response in the ovaries

Zhang

Gui

et al. 2015

BST

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“…Numerous studies indicate that gene expression in CCs can serve as biomarkers for oocyte or embryo quality and pregnancy outcomes 14-16. Our previous self-controlled studies demonstrated the potential anti-apoptotic effect on CCs following DHEA treatment 17, 18. Apoptosis plays a critical role on oogenesis, folliculogenesis, oocyte loss, selection, atresia and luteogenesis 19.…”

Section: Introductionmentioning

confidence: 97%

“…Based on our previous studies 17, 18 and the numerous studies discussed thus far, we hypothesized that DHEA may improve reproductive outcomes in PORs through increasing mitochondrial function and decreasing apoptosis in CCs. To verify this hypothesis, we compared the IVF outcomes of normal ovarian responders (NORs), PORs with or without DHEA supplementation and collected their CCs for researches.…”

Section: Introductionmentioning

confidence: 99%

The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders

Lin¹,

Wang²,

Wen³

et al. 2017

Int. J. Med. Sci.

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Poor ovarian responders (PORs) pose a great challenge for in vitro fertilization (IVF). Previous studies have suggested that dehydroepiandrosterone (DHEA) may improve IVF outcomes in PORs. The current study attempted to investigate the clinical benefits of DHEA in PORs and the possible mechanisms of DHEA on cumulus cells (CCs). This was a prospective study performed at one tertiary center from January 2015 to March 2016. A total of 131 women who underwent IVF treatment participated, including 59 normal ovarian responders (NORs) and 72 PORs. PORs were assigned to receive DHEA supplementation or not before the IVF cycle. For all patients, CCs were obtained after oocyte retrieval. In the CCs, mRNA expression of apoptosis-related genes and mitochondrial transcription factor A (TFAM) gene, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, mitochondrial dehydrogenase activity and mitochondrial mass were measured. The results indicated that PORs with DHEA supplementation produces a great number of top-quality embryos at day 3 and increased the number of transferred embryos and fertilization rate compared with those without DHEA supplementation. Additionally, supplementation with DHEA in PORs decreased DNA damage and apoptosis in CCs while enhancing the mitochondrial mass, mitochondrial dehydrogenase activity and TFAM expression in CCs. In conclusion, our results showed that the benefits of DHEA supplementation on IVF outcomes in PORs were significant, and the effects may be partially mediated by improving mitochondrial function and reducing apoptosis in CCs.

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Gene expression of cumulus cells in women with poor ovarian response after dehydroepiandrosterone supplementation

Abstract: The study showed that DHEA therapy positively affected the gene expression of CCs in women with POR, and provided evidence to support the positive effect of DHEA supplementation on women with POR.

Cited by 30 publications

References 63 publications

Clinical application of dehydroepiandrosterone in reproduction: A review of the evidence

Clinical application of dehydroepiandrosterone in reproduction: A review of the evidence

Dehydroepiandrosterone improves the ovarian reserve of women with diminished ovarian reserve and is a potential regulator of the immune response in the ovaries

The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders

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