Gene expression modulation of ABC transporter genes in response to permethrin in adults of the mosquito malaria vector  Anopheles stephensi

Mastrantonio, Valentina; Ferrari, Marco; Epis, Sara; Negri, Alessandro; Scuccimarra, Giulia; Montagna, Matteo; Favia, Guido; Porretta, Daniele; Urbanelli, Sandra; Bandi, Claudio

doi:10.1016/j.actatropica.2017.03.012

Cited by 24 publications

(29 citation statements)

References 30 publications

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“…sinesis , An. stephensi , and Culex quinquefasciatus [ 23 , 31 , 33 , 62 , 63 , 64 , 65 ]. To the best of our knowledge, this is one of the first documentations showing an association between insecticide resistance and altered mosquito–arbovirus interactions.…”

Section: Discussionmentioning

confidence: 99%

“…Pyrethroid/permethrin resistant mosquitoes exhibit insecticide resistance through elevated levels of multiple detoxification enzymes, including GSTs [ 14 , 20 , 21 , 22 ], ATP-binding cassette (ABC) transporters [ 21 , 23 ], carboxylesterase [ 14 , 24 , 25 ], and cytochrome P450 [ 14 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Penetration resistance occurs through modifications in cuticular proteins [ 14 , 34 ]; metabolic resistance occurs by detoxification enzymes and G-protein-coupled receptor [ 26 ], UDP glucuronosyltransferase, and glucosyl/glucuronosyl transferase [ 32 , 35 ]; and target site insensitivity is mediated by changes in the voltage-gated sodium channel gene [ 11 , 15 , 36 , 37 ], and transcription factor Maf-S [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Permethrin Resistance on Aedes aegypti Transcriptome Following Ingestion of Zika Virus Infected Blood

Zhao

Alto

Shin

et al. 2018

Viruses

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Aedes aegypti (L.) is the primary vector of many emerging arboviruses. Insecticide resistance among mosquito populations is a consequence of the application of insecticides for mosquito control. We used RNA-sequencing to compare transcriptomes between permethrin resistant and susceptible strains of Florida Ae. aegypti in response to Zika virus infection. A total of 2459 transcripts were expressed at significantly different levels between resistant and susceptible Ae. aegypti. Gene ontology analysis placed these genes into seven categories of biological processes. The 863 transcripts were expressed at significantly different levels between the two mosquito strains (up/down regulated) more than 2-fold. Quantitative real-time PCR analysis was used to validate the Zika-infection response. Our results suggested a highly overexpressed P450, with AAEL014617 and AAEL006798 as potential candidates for the molecular mechanism of permethrin resistance in Ae. aegypti. Our findings indicated that most detoxification enzymes and immune system enzymes altered their gene expression between the two strains of Ae. aegypti in response to Zika virus infection. Understanding the interactions of arboviruses with resistant mosquito vectors at the molecular level allows for the possible development of new approaches in mitigating arbovirus transmission. This information sheds light on Zika-induced changes in insecticide resistant Ae. aegypti with implications for mosquito control strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of Permethrin Resistance on Aedes aegypti Transcriptome Following Ingestion of Zika Virus Infected Blood

Zhao

Alto

Shin

et al. 2018

Viruses

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“…In addition, AnstABCB2, AnstABCBmember6, AnstABCG4 were upregulated in male and female adult An. stephensi in response to permethrin insecticide [104]. Both studies indicate that the increased expression of these transporters upon permethrin exposure is crucial for insecticide transport out of the cell and consequently insecticide resistance.…”

Section: Metabolic Resistancementioning

confidence: 95%

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

et al. 2020

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The increasing resistance to currently available insecticides in the malaria vector, Anopheles mosquitoes, hampers their use as an effective vector control strategy for the prevention of malaria transmission. Therefore, there is need for new insecticides and/or alternative vector control strategies, the development of which relies on the identification of possible targets in Anopheles. Some known and promising targets for the prevention or control of malaria transmission exist among Anopheles metabolic proteins. This review aims to elucidate the current and potential contribution of Anopheles metabolic proteins to malaria transmission and control. Highlighted are the roles of metabolic proteins as insecticide targets, in blood digestion and immune response as well as their contribution to insecticide resistance and Plasmodium parasite development. Furthermore, strategies by which these metabolic proteins can be utilized for vector control are described. Inhibitors of Anopheles metabolic proteins that are designed based on target specificity can yield insecticides with no significant toxicity to non-target species. These metabolic modulators combined with each other or with synergists, sterilants, and transmission-blocking agents in a single product, can yield potent malaria intervention strategies. These combinations can provide multiple means of controlling the vector. Also, they can help to slow down the development of insecticide resistance. Moreover, some metabolic proteins can be modulated for mosquito population replacement or suppression strategies, which will significantly help to curb malaria transmission.

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“…Azadirachta indica and other Meliaceae species have shown strong larvicidal, antiemergence, repellency, and antioviposition effects in different mosquito species (Mulla and Su 1999). It is now known that, in several mosquito species, part of the detoxification process against xenobiotics is mediated by adenosine triphosphatebinding cassette (ABC) transporters on Anopheles stephensi Liston (Epis et al 2014a(Epis et al , 2014bPorretta et al 2016;De Marco et al 2017;Mastrantonio et al 2017); Anopheles gambiae Giles (Nkya et al 2014); Aedes aegypti (L.) (Bariami et al 2012); and Aedes albopictus (Skuse) (Esquivel et al 2016). In particular, on An.…”

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confidence: 99%

“…In particular, on An. stephensi it has been demonstrated that, among the 8 subfamilies (from A to H) of ABC transporters existing in insects, the B and G subfamilies play a major role in the detoxification of permethrin, showing a pattern of response that varies with time (Epis et al 2014a(Epis et al , 2014bDe Marco et al 2017;Mastrantonio et al 2017). Despite their importance against pyrethroids, these genes are not differentially expressed in response to larval exposure to temephos, a widely used larvicide, highlighting an insecticide-specific involvement of the transporters in this mosquito species (Porretta et al 2016).…”

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confidence: 99%

Adenosine Triphosphate–Binding Cassette Transporters Are Not Involved In the Detoxification of Azadirachta indica Extracts In Anopheles stephensi Larvae

Ferrari

Negri

Romeo

et al. 2018

Journal of the American Mosquito Control Association

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Detoxifying pathways of mosquitoes against the neem (Azadirachta indica) extracts are still unclear. The aim of the present study was to investigate the role of adenosine triphosphate-binding cassette (ABC) transporters in this process in Anopheles stephensi, one of the main malaria vectors in southern Asia. Third-stage larvae of An. stephensi were fed with fish food alone or in combination with neem extract at 0.5%, 1%, 5%, and 10%. Six ABC-transporter genes from 3 different subfamilies (B, C, and G) were analyzed to assess their relative expression compared with controls. A bioassay was also performed to assess larval mortality rate at different concentrations and in combination with verapamil, an ABC-transporter inhibitor. No significant variation in the expression levels of any transporter belonging to the B, C, and G subfamilies was detected. Furthermore, the use of verapamil did not induce an increase in mortality at any of the tested neem extract concentrations, indicating that ABC transporters are not involved in the detoxification of neem extracts in An. stephensi larvae.

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Gene expression modulation of ABC transporter genes in response to permethrin in adults of the mosquito malaria vector Anopheles stephensi

Cited by 24 publications

References 30 publications

The Effect of Permethrin Resistance on Aedes aegypti Transcriptome Following Ingestion of Zika Virus Infected Blood

The Effect of Permethrin Resistance on Aedes aegypti Transcriptome Following Ingestion of Zika Virus Infected Blood

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

Adenosine Triphosphate–Binding Cassette Transporters Are Not Involved In the Detoxification of Azadirachta indica Extracts In Anopheles stephensi Larvae

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