Gene expression in spermiogenesis

Tanaka, Hiromitsu; Baba, Tadashi

doi:10.1007/s00018-004-4394-y

Cited by 124 publications

(115 citation statements)

References 115 publications

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“…Another special feature of gene expression in round spermatids is the very high level of transcriptional activity that supports the dramatic induction of haploid gene expression. For example, TATA-binding protein, a component of the basic transcription machinery, accumulates at much higher levels in early haploid germ cells than in any somatic cell type, and TFIIB and RNA polymerase II are also overexpressed in the testis (Tanaka & Baba 2005). Therefore, there are high requirements for posttranscriptional mRNA control to secure the correct timing of protein expression and the quality of the extensive mRNA synthesis in male germ cells.…”

Section: The Cb and Mrna Regulation In Spermatogenesismentioning

confidence: 99%

Chromatoid body and small RNAs in male germ cells

Meikar¹,

Ros²,

Korhonen³

et al. 2011

Reproduction

144

154

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The chromatoid body (CB) is a germ granule in the cytoplasm of postmeiotic haploid round spermatids that is loaded with RNA and RNAbinding proteins. Following the discovery of small non-coding RNA-mediated gene regulation and the identification of PIWI-interacting RNAs (piRNAs) that have crucial roles in germ line development, the function of the CB has slowly begun to be revealed. Male germ cells utilise small RNAs to control the complex and specialised process of sperm production. Several microRNAs have been identified during spermatogenesis. In addition, a high number of piRNAs are present both in embryonic and postnatal male germ cells, with their expression being impressively induced in late meiotic cells and haploid round spermatids. At postmeiotic stage of germ cell differentiation, the CB accumulates piRNAs and proteins of piRNA machinery, as well as several other proteins involved in distinct RNA regulation pathways. All existing evidence suggests a role for the CB in mRNA regulation and small RNA-mediated gene control, but the mechanisms remain uncharacterised. In this review, we summarise the current knowledge of the CB and its association with small RNA pathways.

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Section: The Cb and Mrna Regulation In Spermatogenesismentioning

confidence: 99%

Chromatoid body and small RNAs in male germ cells

Meikar¹,

Ros²,

Korhonen³

et al. 2011

Reproduction

144

154

View full text Add to dashboard Cite

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“…Correspondingly, the expression levels of Tnp1, Tnp2, Prm1, Prm2, Tesk1 (testis-specific kinase 1) and Ldhc (lactate dehydrogenase C) increased greatly as testis development proceeded ( Figure S6). Crem has a coactivator in the testis called Fhl5 (four and a half LIM domains 5), which is also a testis-specific transcription factor [51]. Fhl5 was continuously up-regulated during the mouse testis development.…”

Section: Differentially Expressed Transcription Factorsmentioning

confidence: 99%

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

Gong

Pan

Lin

et al. 2012

Sci. China Life Sci.

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Mammalian testis development is a complex and highly sophisticated process. To study the dynamic change of normal testis development at the transcriptional level, we investigated mouse testes at three postnatal ages: 6 days postnatal, 4 weeks old, and 10 weeks old, representing infant (PN1), juvenile (PN2), and adult (PN3) stages, respectively. Using ultra high-throughput RNA sequencing (RNA-seq) technology, we obtained 211 million reads with a length of 35 bp. We identified 18837 genes that were expressed in mouse testes, and found that genes expressed at the highest level were involved in spermatogenesis. The gene expression pattern in PN1 was distinct from that in PN2 and PN3, which indicates that spermatogenesis has commenced in PN2. We analyzed a large number of genes related to spermatogenesis and somatic development of the testis, which play important roles at different developmental stages. We also found that the MAPK, Hedgehog, and Wnt signaling pathways were significantly involved at different developmental stages. These findings further our understanding of the molecular mechanisms that regulate testis development. Our study also demonstrates significant advantages of RNA-seq technology for studying transcriptome during development. next-generation sequencing, transcriptome, mouse testis, development Citation:Gong W, Pan L L, Lin Q, et al. Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing.

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“…Ten regions showed at least one significant gene that was expressed at fourfold or lower in the tumors with losses compared to normal testes. Many of these genes have been reported to be involved in spermatogenesis or have expression restricted to testis (Tanaka and Baba, 2005;DeJong, 2006), including CATSPER3; ROPN1L (ASP) (chr5), ODF2 (chr9), APOA1 (chr11), CCNA1 (chr13), CPEB1, DPP8, RKHD3, and SPESP1 (chr15); and RFPL3S (chr22).…”

Section: Target Gene Identification In Frequently Altered Regionsmentioning

confidence: 99%

In vivo differentiation and genomic evolution in adult male germ cell tumors

Korkola

Heck

Olshen

et al. 2007

Genes Chromosomes & Cancer

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Germ cell tumors (GCTs) are the most common solid malignancy in young adult men, but the genes and genomic regions involved in their etiology are not fully defined. We report here an investigation of DNA copy number changes in GCTs using 1 Mb BAC arrays. As expected, 12p gain was the defining genomic alteration, occurring in 72/74 GCTs. Parallel expression profiling of these tumors identified potential oncogenes from gained regions (LYN and RAB25) and potential tumor suppressor genes in regions of loss (SYNPO2, TTC12, IGSF4, and EPB41L3). Notably, we observed specific genomic alterations associated with histology, including gain of 17p11.2-q21.32 and loss of 2p25.3 in embryonal carcinoma, gain of 8p23.3-12 and loss of 5p15. 33-35.3, 11q23.1-25, and 13q12.11-34 in seminoma, and gain of 1q31.3-42.3, 3p, 14q11.2-32.33, and 20q and loss of 8q11.1-23.1 in yolk sac tumors (YST). Many significant genes that mapped to these regions had previously been associated with specific histologies, such as EOMES (chr3) and BMP2 (chr20) in YST and SPRY2 (chr13) and SOX17 (chr8) in seminomas. Additionally, our results suggest a model in which histologic differentiation of GCTs may drive genomic evolution. V V C 2007 Wiley-Liss, Inc.

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Gene expression in spermiogenesis

Cited by 124 publications

References 115 publications

Chromatoid body and small RNAs in male germ cells

Chromatoid body and small RNAs in male germ cells

Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing

In vivo differentiation and genomic evolution in adult male germ cell tumors

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