Gene expression in response to cyclic mechanical stretch in primary human dermal fibroblasts

Abstract

The human dermal skin is permanently exposed to mechanical stress, for instance during facial expression, which might cause wrinkles with age. Cyclic mechanical stretching of cells results in cellular and cytoskeleton alignment perpendicular to the stretch direction regulating cellular response. With gene expression profiling it was aimed to identify the differentially expressed genes associated with the regulation of the cytoskeleton to investigate the stretch-induced cell alignment mechanism. Here, the transcription activity of the genome in response to cyclic mechanical stress was measured using DNA microarray technology with Agilent SurePrint G3 Human GE 8x60k Microarrays, based on the overall measurement of the mRNA. Gene expression was measured at the beginning of the alignment process showing first reoriented cells after 5 h stretching and at the end after 24 h, where nearly all cells are aligned. Gene expression data of control vs. stretched primary human dermal fibroblasts after 5 h and 24 h demonstrated the regulation of differentially expressed genes associated with metabolism, differentiation and morphology and were deposited at http://www.ncbi.nlm.nih.gov/geo with the accession number GSE58389.

Deoxynivalenol induces structural alterations in epidermoid carcinoma cells A431 and impairs the response to biomechanical stimulation

“…5b,c ). These data are in agreement with previous studies that reported up-regulation of the gene expression of alpha tubulin in dermal fibroblasts after stimulation in similar conditions (16% deformation, 24 h cyclic stretching at 0.5 Hz) 45 . This response seemed to be abolished by pre-incubation with DON (0.1 µM), thus suggesting that even sub-toxic concentrations of the mycotoxin can have an impact on the cellular capability to respond to the uniaxial stretching.…”

“…To this aim, we used a prototype device 44 , designed and built for the application of cyclic uniaxial stretching to cultivated cells. In fact, biomechanical stimulation is known to have a positive impact on the cellular cytoskeleton and trophic stimuli sustaining, among others, cellular proliferation and survival 5 , 45 . In the present study the effect of DON was investigated on the epidermoid carcinoma A431 cells, since, in recent times, DON has been described also for its effects at dermal level 46 , 47 and A431 cells have been extensively used as a model for studies on the effect of natural compounds in human skin cell models 48 , 49 .…”

Identification of Common Hub Genes in Human Dermal Fibroblasts Stimulated by Mechanical Stretch at Both the Early and Late Stages

“…Fibroblasts are one of the main cell types present in the skin; thus, determining the effects of mechanical stretch on HDFs may help to improve our understanding of the mechanisms and assist in promoting expanded skin regeneration. Reichenbach et al ( 7 ) compared stretched HDFs with unstretched HDFs to identify DEGs using mRNA microarray data. However, because of unknown reasons, these authors did not further study or discuss the functions of these DEGs.…”

“…Cyclic stretch was applied using the FX-4000T™ Tension Plus™ System (Flexercell International; McKeesport, PA, USA) with 16% elongation at 0.5 Hz in a half sinus regimen. Forty samples were included in the dataset ( 7 ).…”

Caffeine-free hawk tea lowers cholesterol by reducing free cholesterol uptake and the production of very-low-density lipoprotein

“…The purified total RNA was used to produce Cy3-labeled cDNA using Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s instructions. Agilent SurePrint G3 Human GE 8 × 60 k microarrays were used, and the hybridization was performed according to a previous published protocol 53 . Microarrays were scanned with an Agilent’s Microarray Scanner System, and the resulting images were analyzed with the Agilent Feature Extraction software.…”