Gene expression in plant according to RNAi treatment of the tobacco whitefly

Kim, Jeong-Hee; Seo, Eun-Young; Kim, Jung-Kyu; Lim, Hyoun–Sub; Yu, Yong-Man; Youn, Young‐Nam

doi:10.7744/cnujas.2015.42.2.081

Cited by 2 publications

(2 citation statements)

References 30 publications

(18 reference statements)

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“…Hyalophora cecropia , Spodoptera litura, Plutella xylostella, Spodoptera frugiperda, Choristoneura fumiferana (Bettencourt et al, 2002;Rajagopal et al, 2002, Bautista et al, 2009, Rodriguez-Cabrera et al, 2010, Quan et al ., 2013, Spodoptera litura Bemisia tabaci RNAi (Jeon et al, 2014, Kim et al, 2015.…”

Section: Rnaimentioning

confidence: 99%

Acute Oral Toxicity of dsRNA to Honey Bee, Apis mellifera

Lim¹,

Jung²,

Kim³

et al. 2017

Korean Journal of Environmental Agriculture

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BACKGROUND: RNA interference (RNAi) eliminates or decreases gene expression by disrupting the target mRNA or by interfering with translation. Recently, RNAi technique was applied to generate new crop traits which provide protection against pests. To establish the environmental risk assessment protocol of RNAi LMO in lab scale, we developed dsRNA expression system using E. coli and tested acute oral toxicity assay to honey. METHOD AND RESULTS: The dsRNA expression vector, L4440, was chosen and cloned 240 bp of Snf7 and GFP gene fragment. To develop the maximum dsRNA induction condition in E. coli, we tested induction time, temperature and IPTG concentration in media. To estimate the risk assessment of dsRNA to honey bee, it has been selected and cultured with dsRNA supplement for 48 hours according to OECD guideline. As a result, the optimum condition of dsRNA induction was 37 , 4 hours and 0.4 ℃ mM IPTG concentration and the difference between Snf7 and GFP dsRNA molecules from E. coli was not significant in survival and behavior to honey bee. Furthermore, blast search results indicated that effective match of predicted dsRNA fragments were not existed in honey bee genome. CONCLUSION:In this study, we developed and tested the acute oral toxicity of dsRNA using E. coli expression system to honey bee.

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Section: Rnaimentioning

confidence: 99%

Acute Oral Toxicity of dsRNA to Honey Bee, Apis mellifera

Lim¹,

Jung²,

Kim³

et al. 2017

Korean Journal of Environmental Agriculture

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“…These locations can be enzyme proteins, non-enzyme proteins, cell division mechanism, etc., which are defined as 'sites of action'. Powles and Yu (2010) resistance is based on the mode of action represented by the biochemical mechanism by which an active ingredient affects the target plant (Kim et al, 2015).…”

Section: Types Of Herbicide Resistancementioning

confidence: 99%

Mechanisms of herbicide resistance in weeds

2017

Korean Journal of Agricultural Science

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In major field crops, synthetic herbicides have been used to control weeds worldwide. Globally, herbicide resistance in weeds should be minimized because it is a major limiting factor for food security. Cross resistance can occur with herbicides within the same or in different herbicide families and with the same or different sites of action. Multiple resistance refers to evolved mechanisms of resistance to more than one herbicide (e.g., resistance to both ALS-inhibitors and ACCase-inhibitors) and this resistance was brought about by separate selection processes. Target site resistance could occur from changes at the biochemical site of action of one herbicide. Non target site resistance occurs through mechanisms which reduce the number of herbicide molecules that reach the herbicide target site. There are currently 480 unique cases (species × site of action) of herbicide resistance globally in 252 plant species (145 dicots and 105 monocots). To date, resistance in weeds has been reported to 161 different herbicides, involving 23 of the 26 known herbicide sites of action. Finally, it can be concluded that we can protect crops associated to herbicide resistant weeds by applications of biochemical, genetic and crop control strategies.

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Gene expression in plant according to RNAi treatment of the tobacco whitefly

Cited by 2 publications

References 30 publications

Acute Oral Toxicity of dsRNA to Honey Bee, Apis mellifera

Acute Oral Toxicity of dsRNA to Honey Bee, Apis mellifera

Mechanisms of herbicide resistance in weeds

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