Gene expression in Nicotiana tabacum in response to compatible and incompatible isolates of Pseudomonas solanacearum

Ragueh, Fatima; Lescure, N.; Roby, Dominique; Marco, Yves

doi:10.1016/0885-5765(89)90004-0

Cited by 32 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total RNA extraction was done following either the guanidinium/cesium chloride method adapted by de Tapia et al (1987), or the phenol/chloroform/ isoamylalcohol technique (Ragueh et al 1989). Polyadenylated mRNAs were obtained by chromatographing twice total RNA on oligo-(dT) cellulose as described by Sambrook et al (1989).…”

Section: Methodsmentioning

confidence: 99%

Heavy-metal-responsive genes in maize: identification and comparison of their expression upon various forms of abiotic stress

Didierjean

Frendo

Nasser

et al. 1996

Planta

View full text Add to dashboard Cite

To identify genes involved in defense against heavy-metal stresses, a cDNA library originating from mercuric chloride-treated maize (Zea mays L. cv. INRA 258) leaves was constructed and analysed by differential screening using cDNAs derived from treated and untreated plants. Transcriptionally activated cDNA clones, designated CHEM (chemically-activated), were isolated and characterized. They represent various known proteins, such as glycine-rich proteins, pathogenesis-related proteins, chaperones and membrane proteins. The expression of the genes encoding these proteins was studied in maize subjected to other forms of abiotic stress. Expression of glycine-rich proteins was greatly enhanced by heat stress, and also stimulated by NaCl, polluted rainwater, wounding and cold stress. Pathogenesis-related proteins were strongly induced by ultraviolet light and to a lesser extent by NaCl, polluted rainwater and wounding. Heat-shock protein was mainly induced by heat and cold, and ubiquitin by wounding. Expression of the membrane channel protein was stimulated by heat stress, NaCl, polluted rainwater and ultraviolet-light irradiation.

show abstract

Section: Methodsmentioning

confidence: 99%

Heavy-metal-responsive genes in maize: identification and comparison of their expression upon various forms of abiotic stress

Didierjean

Frendo

Nasser

et al. 1996

Planta

View full text Add to dashboard Cite

show abstract

“…Preliminary experiments using the tobacco-P. solanacearum model system have shown that the HR-inducing isolate, GM11000, has an early and dramatic effect on plant gene expression. Significant changes in the mRNA population are not detected at this stage either with a H R -isolate, GMI1178 or with a compatible isolate, K60 [26]. Here, we describe the isolation of several cDNA clones corresponding to plant mRNAs whose expression has been studied in plants challenged with compatible and incompatible bacterial isolates.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional activation of 2 classes of genes during the hypersensitive reaction of tobacco leaves infiltrated with an incompatible isolate of the phytopathogenic bacterium Pseudomonas solanacearum

et al. 1990

Self Cite

View full text Add to dashboard Cite

Fourteen cDNA clones whose corresponding mRNAs accumulate during the hypersensitive reaction (HR) of tobacco leaves infiltrated with an incompatible strain of the bacterial pathogen Pseudomonas solanacearum have been subdivided by sequence homologies into 6 families. Studies on the accumulation of the mRNAs encoded by these genes in compatible and incompatible plant-bacterial interactions have been carried out and indicate that the 6 cDNA clones can be subdivided into 2 groups. In one group corresponding to 3 cDNA clones, the maximal level of mRNA accumulation is similar in both types of interaction, whereas in the other group, maximal mRNA accumulation in leaves undergoing an HR is 3- to 7-fold higher than in leaves infiltrated with the compatible strain. Within each group, the timing and kinetics of accumulation of the corresponding mRNAs differ for each individual cDNA clone. Run-on experiments indicate that transcriptional activation of these genes plays a major role in the control of their expression. Genomic hybridizations have been performed and indicate that the mRNAs corresponding to the cDNA clones are encoded by multigene families (6 to 20 genes).

show abstract

“…Total RNA was isolated from 5 g of each sample according to the method described by Ragueh et al (1989). After grinding in liquid nitrogen, the material was resuspended in extraction buffer (200 mM Tris, pH 7.0, 20 mM EDTA, 100 mM NaCl, 1% SDS) and extracted twice with phenol:chloroform: isoamylalcohol (25:24:1).…”

Section: Rna Extraction and Purificationmentioning

confidence: 99%

MAIZEWALL. Database and Developmental Gene Expression Profiling of Cell Wall Biosynthesis and Assembly in Maize

Guillaumie¹,

San-Clemente²,

Deswarte³

et al. 2006

Plant Physiology

105

View full text Add to dashboard Cite

An extensive search for maize (Zea mays) genes involved in cell wall biosynthesis and assembly has been performed and 735 sequences have been centralized in a database, MAIZEWALL (http://www.polebio.scsv.ups-tlse.fr/MAIZEWALL). MAIZE-WALL contains a bioinformatic analysis for each entry and gene expression data that are accessible via a user-friendly interface. A maize cell wall macroarray composed of a gene-specific tag for each entry was also constructed to monitor global cell wall-related gene expression in different organs and during internode development. By using this macroarray, we identified sets of genes that exhibit organ and internode-stage preferential expression profiles. These data provide a comprehensive fingerprint of cell wall-related gene expression throughout the maize plant. Moreover, an in-depth examination of genes involved in lignin biosynthesis coupled to biochemical and cytological data from different organs and stages of internode development has also been undertaken. These results allow us to trace spatially and developmentally regulated, putative preferential routes of monolignol biosynthesis involving specific gene family members and suggest that, although all of the gene families of the currently accepted monolignol biosynthetic pathway are conserved in maize, there are subtle differences in family size and a high degree of complexity in spatial expression patterns. These differences are in keeping with the diversity of lignified cell types throughout the maize plant.

show abstract

Gene expression in Nicotiana tabacum in response to compatible and incompatible isolates of Pseudomonas solanacearum

Cited by 32 publications

References 22 publications

Heavy-metal-responsive genes in maize: identification and comparison of their expression upon various forms of abiotic stress

Heavy-metal-responsive genes in maize: identification and comparison of their expression upon various forms of abiotic stress

Transcriptional activation of 2 classes of genes during the hypersensitive reaction of tobacco leaves infiltrated with an incompatible isolate of the phytopathogenic bacterium Pseudomonas solanacearum

MAIZEWALL. Database and Developmental Gene Expression Profiling of Cell Wall Biosynthesis and Assembly in Maize

Contact Info

Product

Resources

About