Gene Expression in Fixed Tissues and Outcome in Hepatocellular Carcinoma

Hoshida, Yujin; Villanueva, Augusto; Kobayashi, Masahiro; Peix, Judit; Chiang, Derek Y.; Camargo, Amy L.; Gupta, Supriya; Moore, Jamie; Wrobel, Matthew J.; Lerner, Jim; Reich, Michael; Chan, Jennifer A.; Glickman, Jonathan N.; Ikeda, Kenji; Hashimoto, Masaji; Watanabe, Goro; Daidone, Maria Grazia; Roayaie, Sasan; Schwartz, Myron; Thung, Swan N.; Salvesen, Helga B.; Gabriel, Stacey; Mazzaferro, Vincenzo; Bruix, Jordi; Friedman, Scott L.; Kumada, Hiromitsu; Llovet, Josep M.; Golub, Todd R.

doi:10.1056/nejmoa0804525

Cited by 1,133 publications

(1,135 citation statements)

References 23 publications

(34 reference statements)

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“…Gene expression data for tumours and adjacent non‐tumour cirrhotic formalin‐fixed, paraffin‐embedded tissues were conducted using the 6k Transcriptionally Informative Gene Panel for DASL (Illumina, GEO‐GPL5474) (Table S1) 14, 15.…”

Section: Methodsmentioning

confidence: 99%

Integrated transcriptomic and proteomic analyses reveal ɑ‐lipoic acid‐regulated cell proliferation via Grb2‐mediated signalling in hepatic cancer cells

Yang

Wang

et al. 2018

J Cellular Molecular Medi

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Hepatocellular carcinoma is the most frequent primary liver cancer worldwide. The use of antioxidants as cancer prevention and treatment agents has become a focus of research in recent years due to their limited adverse effects. Alpha lipoic acid (ɑ‐LA) is synthesized in the liver and is considered a naturally occurring antioxidant. In this study, a total of 4446 differentially expressed genes (2097 down‐regulated and 2349 up‐regulated) were identified via RNA‐Seq in HepG2 cells after exposure to α‐LA for 24 hrs. Moreover, GO and KEGG pathway analyses showed that cancer‐relevant cell membrane proteins were significantly affected. An interaction network analysis predicted that Grb2 might mediate the key target pathways activated by exposure to ɑ‐LA. Verification of the RNA‐Seq and iTRAQ results confirmed that Grb2 mediated the ɑ‐LA‐induced inhibition of cell proliferation in vitro. Furthermore, the analysis of human hepatocellular carcinoma specimens obtained from the GEO database showed that the expression of EGFR and Met correlated with that of Grb2. These findings provide a novel mechanism through which ɑ‐LA regulates cell proliferation via the down‐regulation of growth factor‐stimulated Grb2 signalling.

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Section: Methodsmentioning

confidence: 99%

Integrated transcriptomic and proteomic analyses reveal ɑ‐lipoic acid‐regulated cell proliferation via Grb2‐mediated signalling in hepatic cancer cells

Yang

Wang

et al. 2018

J Cellular Molecular Medi

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“…Post-operative recurrence is common and accounts for the main cause of mortality for HCC patients who underwent resection surgery. Several factors, including tumor size, the number of tumors, presence of venous invasion and degree of liver functional reserve were demonstrated to determine post-operative tumor recurrence [1,2,[7][8][9][10]. Advanced liver fibrosis is one of the most important factors in the development of HCC for patients with chronic hepatitis B (CHB) [11].…”

Section: Introductionmentioning

confidence: 99%

Fibrosis and AST to platelet ratio index predict post-operative prognosis for solitary small hepatitis B-related hepatocellular carcinoma

Hung

Lai

et al. 2010

Hepatol Int

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Purpose Although advanced liver fibrosis is crucial in the development of hepatocellular carcinoma (HCC) for patients with chronic hepatitis B, whether it is associated with the recurrence of HCC after resection remains obscure. This study was aimed to compare the outcomes for patients with minimal or advanced fibrosis in solitary small hepatitis B virus (HBV)-related HCC.Methods This study enrolled 76 patients with small (\5 cm) solitary HBV-related HCC who underwent resection. The outcomes of patients with minimal and advanced fibrosis in non-tumor areas were compared. Serum markers were tested to assess the stage of hepatic fibrosis and to predict prognosis. Results Fourteen patients with an Ishak fibrosis score of 0 or 1 were defined as having minimal fibrosis; the remaining 62 patients were defined as having advanced fibrosis. During a follow-up period of 77.0 ± 50.7 months, 41 patients died. The overall survival rate was significantly higher (P = 0.018) and recurrence rate was lower

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“…Liu and Ye correctly pointed out, weak association of risk score with prognosis in data from Hoshida et al's study might have been the result of a difference in platform and sample sources. 4 Our risk score was developed with data generated from frozen tissues. However, data from GSE10141 were generated by applying complementary DNA-mediated annealing, selection, extension, and ligation technology with the use of fragmented RNAs from formalin-fixed paraffin-embedded tissues.…”

Section: Replymentioning

confidence: 99%

Connectivity-based risk score for hepatocellular carcinoma prognosis

Liu

2013

Hepatology

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Gene Expression in Fixed Tissues and Outcome in Hepatocellular Carcinoma

Cited by 1,133 publications

References 23 publications

Integrated transcriptomic and proteomic analyses reveal ɑ‐lipoic acid‐regulated cell proliferation via Grb2‐mediated signalling in hepatic cancer cells

Integrated transcriptomic and proteomic analyses reveal ɑ‐lipoic acid‐regulated cell proliferation via Grb2‐mediated signalling in hepatic cancer cells

Fibrosis and AST to platelet ratio index predict post-operative prognosis for solitary small hepatitis B-related hepatocellular carcinoma

Connectivity-based risk score for hepatocellular carcinoma prognosis

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