Gene expression in Citrus sinensis (L.) Osbeck following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing Huanglongbing in Florida

Albrecht, Ute; Bowman, Kim D.

doi:10.1016/j.plantsci.2008.05.001

Cited by 190 publications

(225 citation statements)

References 53 publications

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“…PP2 genes have been shown through microarray analyses to be strongly induced in Las infected citrus plants ( ALBRECHT & BOWMAN 2008;KIM et al, 2009;FAN et al, 2011). PP2 genes are encoded by a large gene family with wide distribution in the plant kingdom (DINANT et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

“…However, strong transgene expression without tissue discrimination can result in unnecessary energy expenditure by the plant and possible side effects due to putatively harmful transgene products (ALTPETER et al, 2005;WANG et al, 2005;ZHAO et al, 2004). One method to fine tune transgene expression is to use tissue-specific promoters induced by pathogens or abiotic stresses (BARBOSA-MENDES et al, 2009;DUTT et al, 2012;MIYATA et al, 2012;BENYON et al, 2013;ATTÍLIO et al, 2013;ZOU et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

et al. 2017

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-The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 (Citrus phloem protein 2), known to be expressed in Citrus sinensis phloem. 'Hamlin' sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las. Index terms: Citrus sinensis, Diaphorina citri, genetic transformation, GUS, huanglongbing, quantitative real-time PCR. PROMOTORES DE FLOEMA SÃO DIFERENTEMENTE ATIVADOS POR Candidatus Liberibacter asiaticus EM LARANJEIRAS DOCES TRANSGÊNICASRESUMO -A utilização de promotores preferencialmente expressos em tecidos vegetais específicos é uma estratégia desejável na busca por resistência a patógenos que colonizam tais tecidos. A bacteria Candidatus Liberibacter asiaticus (Las), associada à doença huanglongbing (HLB) em citros, coloniza o tecido floemático. Alguns promotores, além de conferir expressão específica em certos tecidos, podem também responder diferencialmente à presença de patógenos. O objetivo deste estudo foi verificar se a presença de Las poderia modular a ativação dos promotores de floema AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) e CsPP2 (Citrus phloem protein 2), conhecidos por expressão específica em floema de Citrus sinensis. Plantas de laranja 'Hamlin' (Citrus sinensis L. Osbeck) transformadas com o gene reporter uidA (GUS) sob controle dos promotores AtPP2, AtSUC2 e CsPP2 foram infectadas para avaliar a interdependência entre a expressão dos transgenes e concentração de Las. Plantas foram inoculadas com Las por meio de Diaphorina citri e dezoito meses após, a concentração de bactéria e expressão do gene uidA foram determinadas por qPCR e RT-qPCR, respectivamente. A expressão do gene reporter dirigida pelo promotor AtSUC2 foi forte e positivamente correlacionada com a concentração de Las. Portanto, este promotor contém característ...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

et al. 2017

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“…HLB affected fruit do not colour properly. Early flowering in HLB affected plants has also been observed (Albrecht and Bowman, 2008;Martinelli et al, 2012).…”

Section: Introductionmentioning

confidence: 93%

Detection of ‘Candidatus Liberibacter Asiaticus’ the Causal Organism of Huanglongbing, in Mandarin Group of Citrus

Aslam¹,

Khan²,

Rahman³

et al. 2017

IJAB

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Huanglongbing (HLB) is a fatal disease of citrus. We report the detection of 'Candidatus Liberibacter asiaticus' the causal organism of huanglongbing, in mandarin group of citrus. Four genotypes of citrus belonging to mandarin group including: Citrus sunki, Kinnow, Parson's Special and Sun Chu Sha were studied for the detection of HLB bacterium. Taqman based real time qPCR was performed for the detection of Candidatus Liberibacter asiaticus in citrus host. Highest mean cycle threshold value of 25.06 was obtained for Kinnow mandarin while 24.30 for Parson's Special, 23.95 for Citrus Sunki and 22.0 for Sun Chu Sha. These results may be helpful in the development of management plans for HLB control in Pakistan as well as round the world.

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“…2) provided by MapMan [26]. Genes encoding the glycolytic enzymes phosphoglucomutase, glucose-6-phosphate isomerase, and phosphofructokinase were downregulated (Fig.…”

Section: Primary Metabolismmentioning

confidence: 99%

“…Variation among these isoforms could account for unchanged amounts of sucrose, glucose, and fructose in metabolomics results. Starch and sucrose metabolism pathways are also affected by biotic stresses such as Huanglongbing disease [8,[26][27][28] and citrus bacterial canker disease [29] in Citrus (Table S4).…”

Section: Primary Metabolismmentioning

confidence: 99%

Transcriptome and metabolome analysis of Citrus fruit to elucidate puffing disorder

et al. 2014

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a b s t r a c tA systems-level analysis reveals details of molecular mechanisms underlying puffing disorder in Citrus fruit. Flavedo, albedo and juice sac tissues of normal fruits and fruits displaying symptoms of puffing disorder were studied using metabolomics at three developmental stages. Microarrays were used to compare normal and puffed fruits for each of the three tissues. A protein-protein interaction network inferred from previous work on Arabidopsis identified hub proteins whose transcripts show significant changes in expression. Glycolysis, the backbone of primary metabolism, appeared to be severely affected by the disorder, based on both transcriptomic and metabolomic results. Significantly less citric acid was observed consistently in puffed fruits. Gene set enrichment analysis suggested that glycolysis and carbohydrate metabolism were significantly altered in puffed samples in both albedo and flavedo. Expression of invertases and genes for sucrose export, amylose-starch and starch-maltose conversion was higher in puffed fruits. These changes may significantly alter source-sink communications. Genes associated with gibberellin and cytokinin signaling were downregulated in symptomatic albedo tissues, suggesting that these hormones play key roles in the disorder. Findings may be applied toward the development of early diagnostic methods based on host response genes and metabolites (i.e. citric acid), and toward therapeutics based on hormones.

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Gene expression in Citrus sinensis (L.) Osbeck following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing Huanglongbing in Florida

Cited by 190 publications

References 53 publications

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus

Detection of ‘Candidatus Liberibacter Asiaticus’ the Causal Organism of Huanglongbing, in Mandarin Group of Citrus

Transcriptome and metabolome analysis of Citrus fruit to elucidate puffing disorder

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