Gene expression following transfection of fish cells

Béarzotti, M.; Perrot, Elisabeth; Michard-Vanhée, Christine; Jolivet, Geneviève; Attal, J.; Théron, Marie-Claire; Puissant, C.; Dréano, Michel; Kopchick, John J.; Powell, Richard; Gannon, F.; Houdebiné, Louis‐Marie; Chourrout, Daniel

doi:10.1016/0168-1656(92)90015-2

Cited by 34 publications

(16 citation statements)

References 18 publications

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“…Thus, although heterologous sequences, especially viral sequences, continue to be used in fish systems with success (e.g. Volckaert et al, 1994;Winkler et al, 1994;Michard-Vanhee et al, 1994), a number of researchers have recommended the use of fish-derived sequences for appropriately regulated, high levels of transgene expression (Friedenreich and Schartl, 1990;Bearzotti et al, 1992;B6tancourt et al, 1993). In addition to the possibility of sub-optimal/inappropriate expression, the unacceptability to the food market of transgenic fish containing heterologous sequences has resulted in a considerable effort to isolate appropriate fish sequences.…”

Section: Introductionmentioning

confidence: 97%

Regulation and expression of transgenes in fish—a review

Iyengar

Müller

Maclean

1996

Transgenic Research

121

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Transgenic fish, owing to a number of advantages which they offer over other species, are proving to be valuable model systems for the study of gene regulation and development genetics in addition to being useful targets for the genetic manipulation of commercially important traits. Despite having begun only a decade ago, the production of transgenic fish has become commonplace in a number of laboratories world-wide and considerable progress has been made. In this review, we initially consider the various regulatory elements and coding genes which have been used in fish, and subsequently discuss and compare both the transient and long-term fate and expression patterns of injected DNA sequences in the context of the different factors which are likely to have an effect on the expression of transgenes.

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Section: Introductionmentioning

confidence: 97%

Regulation and expression of transgenes in fish—a review

Iyengar

Müller

Maclean

1996

Transgenic Research

121

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“…The copy numbers and integration sites in each type of host cell are not identical (Zhu et al 1989). Transgene expression is regulated by the fusion promoter (Bearzotti et al 1992), and is also restricted by many other unknown factors. These factors may include the flanking sequences of the host chromosome where the transgene integration occurred randomly and the nuclear proteins of the different cell types in which the transgene mosaic is distributed.…”

Section: Discussionmentioning

confidence: 99%

Hormonal replacement therapy in fish:human growth hormone gene function in hypophysectomized carp

Cuil

Zhang

1993

Fish Physiol Biochem

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Transgenic common carp, Cyprinus carpio, produced by the microinjection of fertilized eggs with a linearized chimeric plasmid pMThGH, a human growth hormone (hGH) gene with a mouse metallothionein-1 (MT) gene promoter in pBR322, were used to produce F and F 2 transgenics. Following hypophysectomy of the transgenic F 2 common carp, non-transgenic common carp and non-transgenic crucian carp, growth was monitored for up to 110 days. In addition, recombinant hGH was injected subcutaenously into a group of the non-transgenic crucian carp. Growth rate analyses indicated that (1) hypophysectomy of non-transgenic common carp and crucian carp results in the cessation of growth, (2) hGH administration can stimulate the growth of hypophysectomized crucian carp and (3) hypophysectomized hGH-transgenic common carp continue to grow in the absence of their own growth hormone, suggesting that the hGH-transgene is being expressed in tissues other than the pituitary.

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“…Most of these methods have been used singly and only a few reports describe the combination of these methods to improve e. Modifications in the DNA backbone for optimal transgene delivery The DNA used for transient transfection are plasmids (circular double strain DNA) containing an expression cassette with the transgene and -3 Kb of bacterial sequences to manipulate the construct in bacterias (24). Modifications in the plasmid backbone have been directed to increase transcription by promoter selection (5,41,68) or to increase plasmid replication inside the cells by including replication origins such as the SV40 origin. Improvements of 2-1000-fold in the e have been described when the SV40 early promoter and enhancer were present in the plasmids (19).…”

Section: Cellular Pathway Of the Dna-cationic Liposome Complexes (Dnamentioning

confidence: 99%

Improvement of DNA transfection with cationic liposomes

Rocha

Ruiz

Coll

2002

J. Physiol. Biochem.

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The increasing use of cationic liposomes as vectors for DNA transfection of eukaryotic cells is due to its high efficiency and reproducibility. After the interaction of the DNA cationic-liposome complexes (DNA-CLC) with the plasma membrane, the entry into the cells delivers the DNA-CLC to the endosome-lysosome pathway where some of the DNA-CLC are degraded. The non-degraded DNA that escapes to the cytoplasm, still has to transverse the nuclear membrane to be transcribed and then translated. To improve the efficiency of the whole process, we can manipulate the DNA (sequences, promoters, enhancers, nuclear localisation signals, etc), the DNA-CLC (lipids) or the plasmatic, endosomal and/or nuclear cellular membranes (ultrasound, electroporation, Ca++, pH of the endosomes, mitosis, fusogenic peptides, nuclear localisation signals, etc). Most of these methods have been generally used individually but in combination, may greatly improve the efficiency and reproducibility of in vitro transfection. While much of this work remains yet to be done and present results further explored, the application of these efforts is essential to the future development of new gene therapy strategies.

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Gene expression following transfection of fish cells

Cited by 34 publications

References 18 publications

Regulation and expression of transgenes in fish—a review

Regulation and expression of transgenes in fish—a review

Hormonal replacement therapy in fish:human growth hormone gene function in hypophysectomized carp

Improvement of DNA transfection with cationic liposomes

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