Gene expression endpoints following chronic waterborne copper exposure in a genomic model organism, the zebrafish, Danio rerio

Craig, Paul M.; Högstrand, Christer; Wood, Chris M.; McClelland, Grant B.

doi:10.1152/physiolgenomics.00089.2009

Cited by 44 publications

(19 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While fish can habituate to some chronic stressors, sustained plasma cortisol levels similar to those used in this study have been observed in response to diverse stressors of anthropogenic and natural origins. For example, chronic exposure to aluminum, copper, selenium or low environmental pH can result in prolonged elevations in plasma cortisol levels (Craig et al 2009, Wiseman et al 2011, Kennedy & Picard 2012, Grassie et al 2013. In salmonids, sustained increases in cortisol levels are also associated with social subordination (Gilmour et al 2005) and with specific phases of the life cycle such as smolting (Nilsen et al 2008) and sexual maturation (Fuzzen et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Chronic cortisol and the regulation of food intake and the endocrine growth axis in rainbow trout

Madison

Tavakoli

Krämer

et al. 2015

Journal of Endocrinology

View full text Add to dashboard Cite

To gain a better understanding of the mechanisms by which cortisol suppresses growth during chronic stress in fish, we characterized the effects of chronic cortisol on food intake, mass gain, the expression of appetite-regulating factors, and the activity of the GH/IGF axis. Fish given osmotic pumps that maintained plasma cortisol levels at w70 or 116 ng/ml for 34 days were sampled 14, 28 and 42 days post-implantation. Relative to shams, the cortisol treatments reduced food intake by 40-60% and elicited marked increases in liver leptin (lep-a1) and brain preoptic area (POA) corticotropin-releasing factor (crf) mRNA levels. The cortisol treatments also elicited 40-80% reductions in mass gain associated with increases in pituitary gh, liver gh receptor (ghr), liver igfI and igf binding protein (igfbp)-1 and -2 mRNA levels, reduced plasma GH and no change in plasma IGF1. During recovery, while plasma GH and pituitary gh, liver ghr and igfI gene expression did not differ between treatments, the high cortisol-treated fish had lower plasma IGF1 and elevated liver igfbp1 mRNA levels. Finally, the cortisol-treated fish had higher plasma glucose levels, reduced liver glycogen and lipid reserves, and muscle lipid content. Thus, our findings suggest that the growth-suppressing effects of chronic cortisol in rainbow trout result from reduced food intake mediated at least in part by increases in liver lep-a1 and POA crf mRNA, from sustained increases in hepatic igfbp1 expression that reduce the growth-promoting actions of the GH/IGF axis, and from a mobilization of energy reserves.

show abstract

Section: Discussionmentioning

confidence: 99%

Chronic cortisol and the regulation of food intake and the endocrine growth axis in rainbow trout

Madison

Tavakoli

Krämer

et al. 2015

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…These changes may be the result of a common generalized stress response in fishes to metal ion toxicity (Craig et al, 2009) or in response to systemic hypoxia as a result of impaired gas transport at the gills. Increases in Hct and Hb could occur to elevate the blood oxygen carrying capacity (Larsson et al, 1985;Cyriac et al, 1989) and to increase extraction at the gills.…”

Section: Hematological Parametersmentioning

confidence: 99%

Oxidative stress and metabolic responses to copper in freshwater- and seawater-acclimated killifish, Fundulus heteroclitus

et al. 2015

Self Cite

View full text Add to dashboard Cite

“…Indeed, corticosteroids are believed to be endocrine regulators of some immune components and mechanisms in the short term (Kawano et al 2003;Esteban et al 2004). In contrast, chronic toxic exposure may be linked to long-term activation of the corticotropic axis (Craig et al 2009), reflected by immunosuppression. Furthermore, there is evidence for molecular interactions between the sex-steroid pathways and the corticotropic axis in fish (Milla et al 2009).…”

Section: Future Directionsmentioning

confidence: 99%

The effects of estrogenic and androgenic endocrine disruptors on the immune system of fish: a review

2011

View full text Add to dashboard Cite

During the last decade, a number of studies have shown that, in addition to their classically described reproductive function, estrogens and androgens also regulate the immune system in teleosts. Today, several molecules are known to interfere with the sex-steroid signaling. These chemicals are often referred to as endocrine disrupting contaminants (EDCs). We review the growing evidence that these compounds interfere with the fish immune system. These studies encompass a broad range of approaches from field studies to those at the molecular level. This integrative overview improves our understanding of the various endocrine-disrupting processes triggered by these chemicals. Furthermore, the research also explains why fish that have been exposed to EDCs are more sensitive to pathogens during gametogenesis. In this review, we first discuss the primary actions of sex-steroid-like endocrine disruptors in fish and the specificity of the fish immune system in comparison to mammals. Then, we review the known interactions between the immune system and EDCs and interpret the primary effects of sex steroids (estrogens and androgens) and their related endocrine disruptors on immune modulation. The recent literature suggests that immune parameters may be used as biomarkers of contamination by EDCs. However, caution should be used in the assessment of such immunotoxicity. In particular, more attention should be paid to the specificity of these biomarkers, the external/internal factors influencing the response, and the transduction pathways induced by these molecules in fish. The use of the well-known mammalian models provides a useful guide for future research in fish.

show abstract

Gene expression endpoints following chronic waterborne copper exposure in a genomic model organism, the zebrafish, Danio rerio

Abstract: Craig PM, Hogstrand C, Wood CM, McClelland GB. Gene expression endpoints following chronic waterborne copper exposure in a genomic model organism, the zebrafish, Danio rerio. Physiol Genomics 40: 23

Cited by 44 publications

References 67 publications

Chronic cortisol and the regulation of food intake and the endocrine growth axis in rainbow trout

Chronic cortisol and the regulation of food intake and the endocrine growth axis in rainbow trout

Oxidative stress and metabolic responses to copper in freshwater- and seawater-acclimated killifish, Fundulus heteroclitus

The effects of estrogenic and androgenic endocrine disruptors on the immune system of fish: a review

Contact Info

Product

Resources

About