Gene expression and the diversity of polysomal messenger RNA sequences in regenerating liver.

Scholla, Clara Ann; Tedeschi, M.Valeria; Fausto, Nelson

doi:10.1016/s0021-9258(19)85818-0

Cited by 25 publications

(8 citation statements)

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“…The average length of the mRNA obtained from normal, regenerating liver and tumor mRNA was approximately 1500-1800 nucleotides. These values are similar to those reported previously by Atryzek & Fausto (1979), Reiners & Busch (1980), and Scholia et al (1980). The size of the cDNAs transcribed was determined as previously described (Colbert et al, 1977;Scholia et al, 1980).…”

Section: Resultssupporting

confidence: 84%

“…of normal and regenerating liver are similar (Colbert et al, 1977), and there is extensive, if not complete, homology between these mRNA populations (Wilkes et al, 1979;Scholia et al, 1980). Thus, although there is a large increase in the amount of polysomal poly(A)+ mRNA in regenerating liver (Atryzek & Fausto, 1979), qualitative changes in polysomal mRNA have not been demonstrated in the most recent work (Wilkes et al, 1979;Scholia et al, 1980). Atryzek et al (1980) in one of our laboratories investigated the characteristics of polysomal poly(A)+ mRNA populations in livers of rats kept on a carcinogenic diet which contains ethionine and is choline deficient.…”

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“…Isolation of Total Polysomes. Total polysomes from normal and regenerating liver were isolated exactly as previously described (Scholia et al, 1980). For the isolation of polysomes from hepatoma, the tumors were excised immediately after decapitation and rapidly washed, cleaned, and minced in cold solution H [25 mM Tris (pH 7.4), 250 mM KC1, 40 mM NaCl, 7.5 mM MgCl2, 6 mM 2-mercaptoethanol, 250 Mg/mL heparin, and 0.25 M sucrose].…”

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confidence: 99%

“…Extraction of Polysomal Polyadenylated mRNA. Extraction of RNA from all preparations, with the exception of tumor total polysomes, and isolation of polyadenylated RNA on poly(U)-Sepharose columns were exactly as previously described (Scholia et al, 1980). The extraction procedure was slightly modified for tumor total polysomes because they were not pelleted.…”

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confidence: 99%

“…[3H]cDNAs complementary to polyadenylated RNAs from total and free polysomes from tumor tissue and from normal and regenerating liver were synthesized exactly as previously described (Scholia et al, 1980) by using avian myeloblastosis virus reverse transcriptase (gift of Dr. J. W. Beard, Life Sciences Inc.). The labeled cDNA was passed through a column of Sephadex G-50 over Chelex 100.…”

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Alterations in polyadenylated messenger ribonucleic acid from free and total polysomes of rat hepatoma

Scholla¹,

Petropoulos²,

Becker³

et al. 1981

Biochemistry

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We examined the homology between polysomal polyadenylated ribonucleic acid (mRNA) populations of hepatoma 252, a tumor which is deficient in the synthesis of plasma proteins, and those of normal and regenerating rat liver. Hybridization of polyadenylated mRNA populations with homologous or heterologous complementary deoxyribonucleic acids showed that mRNA from total and free polysomes from hepatoma 252 lack sequences which are present in normal or regenerating liver. Although there are obvious differences in the abundance of sequences between tumor and normal or regenerating liver polysomal mRNA, we did not detect, with the techniques used in this work, tumor-specific sequences. Analysis of hybridization curves using derivative plots did not reveal the presence in tumor mRNA of a high complexity class not present in normal liver. We conclude that alterations in mRNA populations of free and total polysomes of this tumor primarily reflect processes of genetic restriction rather than the derepression of previously unexpressed genes.

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Section: Resultssupporting

confidence: 84%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Alterations in polyadenylated messenger ribonucleic acid from free and total polysomes of rat hepatoma

Scholla¹,

Petropoulos²,

Becker³

et al. 1981

Biochemistry

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Oncogene Expression in Liver Regeneration and Hepatocarcinogenesis

2007

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Studies of gene transcription and translation in regenerating rat liver

et al. 1986

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Specific transcriptional and translational products associated with regenerating liver were analyzed by differential hybridization to a cDNA library and by two-dimensional electrophoresis of hepatic proteins, respectively. Comparisons of approximately 800 soluble and 800 particulate liver proteins from normal and 70% partially hepatectomized Fischer rats resulted in the identification of only three apparently unique polypeptides in 70% partially hepatectomized livers, although many quantitative changes were observed. A subset of these quantitative changes were also observed after sham operation. A cDNA library was generated from polyadenylated RNA isolated 18 hr post-70% partial hepatectomy. Comparative analysis of 6,000 transformants with single-stranded cDNA probes prepared from 18 hr post-70% partial hepatectomy and sham-operated animals identified three clones whose sequences were preferentially expressed 4- to 6-fold 18 hr post-70% partial hepatectomy. Southern blot analysis of one clone, REG-A, showed no homology to albumin, alpha-fetoprotein, three different forms of cytochrome P-450, ornithine decarboxylase, globin, or to a putative tumor promotion associated gene called PRO-2. A single, REG-A specific 2.5 kb band was identified by Northern blot analysis of liver samples. REG-A expression was increased 2-fold 18 hr postsham operation; 4-fold 18 hr post-70% partial hepatectomy and following chronic 2,3,7,8-tetrachlorodibenzo-p-dioxin or phenobarbital treatment. REG-A expression returned to control levels 1 week after 70% partial hepatectomy. Furthermore, expression of REG-A was reduced in chemically induced preneoplastic nodules and in primary and transplantable hepatomas. Hybrid selection studies indicated that the REG-A sequence selected a mRNA(s) species, that in an in vitro translation assay, produced two major polypeptides of 21,000 and 25,000 molecular weight with a pI of 6.9. Thus, these data support the hypothesis that liver regeneration is characterized by quantitative changes in genes normally expressed at low levels in the Go hepatocyte and is not the result of major qualitative changes in gene expression.

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Gene expression and the diversity of polysomal messenger RNA sequences in regenerating liver.

Cited by 25 publications

References 23 publications

Alterations in polyadenylated messenger ribonucleic acid from free and total polysomes of rat hepatoma

Alterations in polyadenylated messenger ribonucleic acid from free and total polysomes of rat hepatoma

Oncogene Expression in Liver Regeneration and Hepatocarcinogenesis

Studies of gene transcription and translation in regenerating rat liver

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