Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinities

Inokuchi, Mayu; Hiroi, Junya; Watanabe, Shinji; Lee, Kyung Mi; Kaneko, Toyoji

doi:10.1016/j.cbpa.2008.06.012

Cited by 139 publications

(131 citation statements)

References 30 publications

Supporting

Mentioning

111

Contrasting

Order By: Relevance

“…Recently, a series of studies has elucidated the sub-cellular components of FW-type MRCs in Mozambique tilapia , Inokuchi et al 2008. These studies described the expression of either a sodium proton exchanger type 3 (nhe3) or a sodium chloride cotransporter (ncc) in two distinct FW-type MRCs.…”

Section: Discussionmentioning

confidence: 99%

Switching of Na+, K+-ATPase isoforms by salinity and prolactin in the gill of a cichlid fish

Tipsmark

Breves²,

Seale³

et al. 2011

Journal of Endocrinology

105

View full text Add to dashboard Cite

We identified and investigated the changes in expression of two gill Na C , K C -ATPase a-subunit isoforms (a-1a and a-1b) in relationship with salinity acclimation in a cichlid fish, Mozambique tilapia. Transfer of freshwater (FW)-acclimated fish to seawater (SW) resulted in a marked reduction in a-1a expression within 24 h and a significant increase in a-1b expression with maximum levels attained 7 days after the transfer. In contrast, transfer of SWacclimated fish to FW induced a marked increase in a-1a expression within 2 days, while a-1b expression decreased significantly after 14 days. Hypophysectomy resulted in a virtual shutdown of a-1a mRNA expression in both FW-and SW-acclimated fish, whereas no significant effect was observed in a-1b expression. Replacement therapy by ovine prolactin (oPrl) fully restored a-1a expression in FW-acclimated fish, while cortisol had a modest, but significant, stimulatory effect on a-1a expression. In hypophysectomized fish in SW, replacement therapy with oPrl alone or in combination with cortisol resulted in a marked increase in a-1a mRNA to levels far exceeding those observed in sham-operated fish. Expression of a-1b mRNA was unaffected by hormone treatment either in FW-acclimated fish or in SW-acclimated fish. The mRNA expression of fxyd-11, a regulatory Na C , K C -ATPase subunit, was transiently enhanced during both FW and SW acclimation. In hypophysectomized fish in FW, oPrl and cortisol stimulated fxyd-11 expression in a synergistic manner. The clear Prl dependence of gill a-1a expression may partially explain the importance of this hormone to hyperosmoregulation in this species.

show abstract

Section: Discussionmentioning

confidence: 99%

Switching of Na+, K+-ATPase isoforms by salinity and prolactin in the gill of a cichlid fish

Tipsmark

Breves²,

Seale³

et al. 2011

Journal of Endocrinology

105

View full text Add to dashboard Cite

show abstract

“…Hiroi and coworkers cloned an orthologue of zNCCg from tilapia and used a homologous antibody to specifically localize the NCC isoform in the apical membrane of type-II MR cells but not in other MR cell types Inokuchi et al, 2008). Acclimation to FW or deionized FW caused an increase in the density of NCC-expressing type-II MR cells and a concomitant upregulation of NCC mRNA in tilapia adult gills and embryonic yolk-sac membranes, compared with the SW controls Inokuchi et al, 2008). Hiroi and colleagues' studies could not distinguish between the uptake of Na + , Cl -or both ions as the role of NCC in tilapia.…”

Section: Ncc Cells Apical Nccmentioning

confidence: 99%

Ion uptake and acid secretion in zebrafish (Danio rerio)

Hwang

2009

Journal of Experimental Biology

166

165

View full text Add to dashboard Cite

SummaryTransepithelial transport is one of the major processes involved in the mechanism of homeostasis of body fluids in vertebrates including fish. The current models of ion regulation in fish gill ionocytes have been proposed mainly based on studies in traditional model species like salmon, trout, tilapia, eel and killifish, but the mechanisms are still being debated due to the lack of convincing molecular physiological evidence. Taking advantage of plentiful genetic databases for zebrafish, we studied the molecular/cellular mechanisms of ion regulation in fish skin/gills. In our recently proposed model, there are at least three subtypes of ionocytes in zebrafish skin/gills: Na + -K + -ATPase-rich (NaR), Na + -Cl -cotransporter (NCC) and H + -ATPase-rich (HR) cells. Specific isoforms of transporters and enzymes have been identified as being expressed by these ionocytes: zECaC, zPMCA2 and zNCX1b by NaR cells; zNCC gill form by NCC cells; and zH + -ATPase, zNHE3b, zCA2-like a and zCA15a by HR cells. Serial molecular physiological experiments demonstrated the distinct roles of these ionocytes in the transport of various ions: HR, NaR and NCC cells are respectively responsible for acid secretion/Na + uptake, Ca 2+ uptake and Cl -uptake. The expression, regulation and function of transporters in HR and NaR cells are much better understood than those in NCC cells. The basolateral transport pathways in HR and NCC cells are still unclear, and the driving forces for the operations of apical NHE and NCC are another unresolved issue. Studies on zebrafish skin/gill ionocytes are providing new insights into fish ion-regulatory mechanisms, but the zebrafish model cannot simply be applied to other species because of species differences and a lack of sufficient molecular physiological evidence in other species.

show abstract

“…The NKCC1a was termed a secretory type based on its higher level in tilapia gill after SW transfer , Inokuchi et al 2008. Only one NKCC1 (partial) sequence has been described in striped bass (Tipsmark et al 2004), and a BLAST search suggests this to be the secretory NKCC1a isoform.…”

Section: Kmentioning

confidence: 99%

“…The different acclimation 'strategies' are also evident in the endocrine response when the two species are challenged with changing salinity. In tilapia, direct transfer from fresh water (FW)-to seawater (SW) results in drastically increased levels of plasma cortisol, and subsequently chloride cell number, NKCC1a and Na C ,K C -ATPase mRNA, protein and activity levels in the gill (Assem & Hanke 1981, Morgan et al 1997, Hwang et al 1998, Inokuchi et al 2008. Accordingly, cortisol seems to be an important osmoregulatory hormone in tilapia, where it stimulates Na C ,K C -ATPase activity and chloride cell proliferation and differentiation (Dangé 1986, McCormick 1990.…”

Section: Introductionmentioning

confidence: 99%

Differential effects of cortisol and 11-deoxycorticosterone on ion transport protein mRNA levels in gills of two euryhaline teleosts, Mozambique tilapia (Oreochromis mossambicus) and striped bass (Morone saxatilis)

Kiilerich¹,

Tipsmark²,

Borski³

et al. 2011

Journal of Endocrinology

View full text Add to dashboard Cite

The role of cortisol as the only corticosteroid in fish osmoregulation has recently been challenged with the discovery of a mineralocorticoid-like hormone, 11-deoxycorticosterone (DOC), and necessitates new studies of the endocrinology of osmoregulation in fish. Using an in vitro gill explant incubation approach, DOC-mediated regulation of selected osmoregulatory target genes in the gill was investigated and compared with that of cortisol in two euryhaline teleosts, Mozambique tilapia (Oreochromis mossambicus) and striped bass (Morone saxatilis). The effects were tested in gills from both fresh water (FW)-and seawater (SW)-acclimated fish. Both cortisol and DOC caused an up-regulation of the Na C ,K C -ATPase a1 subunit in SW-acclimated tilapia but had no effect in FW-acclimated fish. Cortisol conferred an increase in NaK cotransporter (NKCC) isoform 1a transcript levels in FW-and SW-acclimated tilapia, whereas DOC had a stimulatory effect only in SW-acclimated fish. Cortisol had no effect on NKCC isoform 1b mRNA levels at both salinities, while DOC stimulated this isoform in SW-acclimated fish. In striped bass, cortisol conferred an up-regulation of Na C ,K C -ATPase a1 and NKCC transcript levels in FW-and SW-acclimated fish, whereas DOC resulted in down-regulation of these transcripts in FW-acclimated fish. It was also found that both corticosteroids may rapidly (30 min) alter the mitogen-activated protein kinase signalling pathway in gill, inducing phosphorylation of extracellular signalregulated kinase 1 (ERK1) and ERK2 in a salinity-dependent manner. The study shows a disparate organisation of corticosteroid signalling mechanisms involved in ion regulation in the two species and adds new evidence to a role of DOC as a mineralocorticoid hormone in teleosts.

show abstract

Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinities

Cited by 139 publications

References 30 publications

Switching of Na+, K+-ATPase isoforms by salinity and prolactin in the gill of a cichlid fish

Switching of Na+, K+-ATPase isoforms by salinity and prolactin in the gill of a cichlid fish

Ion uptake and acid secretion in zebrafish (Danio rerio)

Differential effects of cortisol and 11-deoxycorticosterone on ion transport protein mRNA levels in gills of two euryhaline teleosts, Mozambique tilapia (Oreochromis mossambicus) and striped bass (Morone saxatilis)

Contact Info

Product

Resources

About