Gene expression and immunolocalization of amelogenin in enamel hypoplasia induced by successive injections of bisphosphonate in rat incisors

Yamada, Yuya; Fuangtharnthip, Pornpoj; Tamura, Yukihiko; Takagi, Yasuaki; Ohya, Keiichi

doi:10.1016/s0003-9969(99)00134-x

Cited by 17 publications

(23 citation statements)

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“…Fejerskov et al (1990) De fato, os ameloblastos no estágio de maturação são essenciais para proporcionar a mineralização do esmalte, provavelmente controlando o influxo de minerais ou criando condições físicas para a precipitação de hidroxiapatita de uma maneira bastante controlada . Yamada et al (2000) propuseram elucidar a patogênese da hipoplasia de esmalte induzida por etidronato e, especialmente, determinar que processo inibitório da síntese e secreção de proteína está envolvido. Com base nos resultados obtidos, os autores sugeriram que a hipoplasia de esmalte nos ratos tratados com a droga não foram relacionadas a um distúrbio na síntese de amelogenina, mas a um distúrbio em um processo posterior, presumivelmente de secreção das proteínas do esmalte.…”

Section: Discussionunclassified

“…Essas observações refletem a inibição do processo de secreção protéica, causado de alguma forma, por sucessivas injeções de etidronato Yamada et al, 2000).…”

Section: Discussionunclassified

“…Além disso, nos estudos que verificaram alterações nas estruturas dentárias, após uso do etidronato, (Ogawa et al, 1989;Josephsen et al, 1990;Fejerskov et al, 1990;Weile et al, 1990;Fuangtharnthip et al, 2000;Yamada et al, 2000), o regime utilizado foi a injeção de uma dose única ou a administração diária da droga durante um curto intervalo de tempo.…”

Section: Discussionunclassified

“…O etidronato é um bisfosfonato não aminado, diferente do alendronato, um aminobisfosfonato, que possui elevada afinidade mineral e potente atividade antirreabsortiva, dada a sua capacidade de afetar processos celulares bioquímicos, como a inibição da enzima farnesil pirofosfato sintase (FPPS) (Russell et al, 2008). Tais características podem ser utilizadas também para justificar os resultados do presente estudo, que divergem da maioria dos achados de outros pesquisadores, que demonstraram diversos distúrbios nos tecidos dentários mineralizados, incluindo hipomineralização, hipoplasias e cistos, quando utilizaram o etidronato (Beertsen et al, 1985;Simmelink, 1987;Ogawa et al, 1989;Fejerskov et al, 1990;Josephsen et al, 1990;Weile et al, 1990;Fouda et al, 1991;Weile et al, 1993;Takano et al, 1998;Sakai et al, 1999;Fuangtharnthip et al, 2000;Yamada et al, 2000;Ohma et al, 2000). No presente estudo, não foi possível observar qualquer efeito significativo do alendronato sódico na formação do esmalte e da dentina de incisivos de ratos, por meio das metodologias empregadas.…”

Section: Discussionunclassified

“…Foi relatado também que ratos injetados com etidronato (HEBP) (8mgP/Kg/dia), por 7 dias, desenvolveram hipoplasia de esmalte, devido a um distúrbio na secreção de proteínas Yamada et al, 2000).…”

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Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

Lucisano¹

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Section: Discussionunclassified

“…Essas observações refletem a inibição do processo de secreção protéica, causado de alguma forma, por sucessivas injeções de etidronato Yamada et al, 2000).…”

Section: Discussionunclassified

Section: Palavrasunclassified

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Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

Lucisano¹

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Systemically alendronate was incorporated into dental tissues but did not cause morphological or mechanical changes in rats teeth

Filho

Lucisano

Silva

et al. 2012

Microscopy Res & Technique

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2012Systemically alendronate was incorporated into dental tissues but did not cause morphological or mechanical changes in rats teeth MICROSCOPY RESEARCH AND TECHNIQUE, HOBOKEN, v. 75, n. 9, pp. 1265-1271, SEP, 2012 http://www.producao.usp.br/handle/BDPI/42701 ABSTRACTThis study evaluated the effect of the systemic use of sodium alendronate in rats in vivo. Forty-five Wistar rats aged 36 to 42 days and weighing 200 to 230 g were randomly assigned to a control group (n 5 20), which received distilled water, and an experimental group (n 5 25), which received 2 weekly doses of 1 mg/kg of chemically pure sodium alendronate. The animals were killed after 60 days of treatment. The tibias were removed for analysis of bone mineral density by dual-energy X-ray absorptiometry (DXA). Then, the maxillary incisors were extracted for analysis of the mineralized dental tissues using fluorescence spectroscopy (FS), scanning electron microscopy (SEM), bright field microscopy (BFM), and cross-sectional microhardness (CSMH) testing. DXA and CSMH data were subjected to statistical analysis by Kruskal-Wallis test (5% significance level). The experimental group presented higher bone mineral density than the control group by DXA. FS analysis revealed presence of alendronate in the mineralized dental tissues of the specimens of the experimental group. Significant morphological differences were not found by SEM and BFM. Enamel and dentin (100 and 300 lm from the dentinoenamel junction) CSMH data did not show significant difference between the control and experimental groups. Based on the obtained results, we conclude that while alendronate increased the bone mineral density and was incorporated into the mineralized dental tissues it did not cause significant alterations in the morphology and microhardness of rat incisor enamel and dentin. Microsc. Res. Tech. 75:1265-1271, 2012

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Administration of the Bisphosphonate Zoledronic Acid During Tooth Development Inhibits Tooth Eruption and Formation and Induces Dental Abnormalities in Rats

et al. 2010

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Bisphosphonates (BPs) are potent inhibitors of osteoclastic bone resorption and widely used for the treatment of osteoporosis and metastatic bone diseases. Recently, BPs have also been shown to benefit children with primary and secondary osteoporosis, including osteogenesis imperfecta; however, their long-term safety has not been established yet. Clinical and experimental studies have demonstrated that BPs delay or inhibit tooth eruption. The failure of tooth eruption causes several dental abnormalities. In this study, to determine the effects of BPs on tooth formation, the BP zoledronic acid (ZOL) was injected into 7- and 14-day-old rats, and the development of the mandibular teeth was examined. X-ray analysis demonstrated that ZOL inhibited the eruption of both incisors and molars and their formation, especially in the molar roots. Histological examination showed that, in ZOL-treated animals, alveolar bone remained unresorbed around tooth crowns, which injured ameloblasts and enamel matrix, leading to defects of the enamel. Furthermore, haphazard proliferation of odontogenic epithelium and mesenchyme associated with primitive tooth structures, which resembles human odontomas, was induced at the basal end of incisors but not around the molars. Tooth ankylosis to alveolar bone was occasionally observed in molars. These results suggest that administration of BPs during tooth development has the potential to inhibit tooth eruption and formation and to induce several types of dental abnormalities, which may be attributed to the altered osteoclastic activities.

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Gene expression and immunolocalization of amelogenin in enamel hypoplasia induced by successive injections of bisphosphonate in rat incisors

Cited by 17 publications

References 40 publications

Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

Systemically alendronate was incorporated into dental tissues but did not cause morphological or mechanical changes in rats teeth

Administration of the Bisphosphonate Zoledronic Acid During Tooth Development Inhibits Tooth Eruption and Formation and Induces Dental Abnormalities in Rats

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