Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.)

Ba, Qingsong; Zhang, Gaisheng; Wang, Junsheng; Niu, Na; Ma, Shen; Wang, Junwei

doi:10.1007/s11738-013-1431-6

Cited by 35 publications

(29 citation statements)

References 43 publications

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“…Some studies have found altered methylation patterns in male sterile plants and evidence that methylation regulates EGMS in rice [23,24,25], CMS in rice [26] and maize [27] and GMS in tomato [28] and wheat [29]. However, to date, no study has reported these methylation profiles and their possible roles in the cabbage male sterility line.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Han

Zhang

Liu

et al. 2017

Genes

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Methylation modifications play an important role in multiple biological processes. Several studies have reported altered methylation patterns in male sterile plants such as rice and wheat, but little is known about the global methylation profiles and their possible roles in the cabbage (Brassica oleracea) male sterile line. In this study, single-base-resolution bisulfite sequencing (BS-Seq) was adopted to identify the pattern and degree of cytosine methylation in the male sterile line 01-20S and its near-isogenic fertile line 01-20F. Similar methylation patterns were profiled, with some changes observed in local positions. In total, 505 differentially methylated genomic regions (DMRs) and 106 DMR-associated genes were detected. Nine genes related to pollen development were discovered and further validated by a quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Among these, four were downregulated in 01-20S. In particular, Bol039180 (an invertase/pectin methylesterase inhibitor family protein) is likely involved in pectin degradation, and might play an important role in the pollen separation defects of 01-20S. This study facilitates a better understanding of DNA methylation alterations and their possible roles in genic male sterility in cabbages.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Han

Zhang

Liu

et al. 2017

Genes

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“…According to the morphological and cytological characteristics observed by light microscopy and previous classifications of microsporogenesis (Ba et al, 2014), we divided the wheat microsporogenesis process into five stages. The anthers of the sterile plants appeared to be normal during the first few stages.…”

Section: Phenotypic Characteristicsmentioning

confidence: 99%

A Sterility Induction Trait in the Genic Male Sterility Wheat Line 4110S Induced by High Temperature and its Cytological Response

et al. 2018

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Male sterility has an essential role to exploit heterosis. In particular, the development of the photo‐thermosensitive male sterility system has accelerated hybrid wheat (Triticum aestivum L.) breeding. In this study, we obtained a novel thermosensitive genic male sterility (TGMS) wheat line 4110S, where its fertility was sensitive to temperature during the later uninucleate stage and the line was completely male sterile when the average temperature exceeded ∼20°C. Phenotypic and cytological analyses detected morphologically distinct hallmarks of fertility‐to‐sterility conversion in the anther during the trinucleate stage. Delayed programmed cell death in the tapetum affected microspore development and caused defective pollen exine formation, which ultimately resulted in male sterility. The TGMS line could be used in the two‐line hybrid production systems, where it can maintain male sterility via self‐pollination at low temperatures and can be crossed with a pollen donor to produce hybrid wheat seeds at higher temperatures. Our findings provide a better understanding of the mechanisms responsible for male sterility in wheat, thereby facilitating production of hybrid wheat seed.

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“…Based on morphological landmarks or cellular events observed by light microscopy and according to a previous classification of anther development (Zhang et al, 2013; Ba et al, 2014), we assigned wheat anther development to five stages. The results showed that the AS anthers appeared to be normal in the first few stages ( Figures 1A–D,H–K ), but there were some differences in anther morphology between AF and AS during the trinucleate stage.…”

Section: Resultsmentioning

confidence: 99%

“…Before the introduction of high-throughput sequencing technology, two-dimensional gel electrophoresis (Li et al, 2011), DDRT-PCR (Zhao et al, 2007), SSH (Li et al, 2008), and cDNA-AFLP (Song et al, 2006) were employed to obtain the gene expression profiles related to male sterility in wheat. The mechanism of male sterility has also been studied with respect to polyubiquitin-related protein and DNA methylation (Ba et al, 2014; Liu et al, 2014). However, the transcription profiles of cytoplasmic male sterile wheat have been investigated little.…”

Section: Introductionmentioning

confidence: 99%

Identification of Candidate Genes and Biosynthesis Pathways Related to Fertility Conversion by Wheat KTM3315A Transcriptome Profiling

Duan

Gan

et al. 2017

Front. Plant Sci.

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The Aegilops kotschyi thermo-sensitive cytoplasmic male sterility (K-TCMS) system may facilitate hybrid wheat (Triticum aestivum L.) seed multiplication and production. The K-TCMS line is completely male sterile during the normal wheat-growing season, whereas its fertility can be restored in a high-temperature environment. To elucidate the molecular mechanisms responsible for male sterility/fertility conversion and candidate genes involved with pollen development in K-TCMS, we employed RNA-seq to sequence the transcriptomes of anthers from K-TCMS line KTM3315A during development under sterile and fertile conditions. We identified 16840 differentially expressed genes (DEGs) in different stages including15157 known genes (15135 nuclear genes and 22 plasmagenes) and 1683 novel genes. Bioinformatics analysis identified possible metabolic pathways involved with fertility based on KEGG pathway enrichment of the DEGs expressed in fertile and sterile plants. We found that most of the genes encoding key enzyme in the phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were significant upregulated in uninucleate, binuclate or trinucleate stage, which both interact with MYB transcription factors, and that link between all play essential roles in fertility conversion. The relevant DEGs were verified by quantitative RT-PCR. Thus, we suggested that phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were involved in fertility conversion of K-TCMS wheat. This will provide a new perspective and an effective foundation for the research of molecular mechanisms of fertility conversion of CMS wheat. Fertility conversion mechanism in thermo-sensitive cytoplasmic male sterile/fertile wheat involves the phenylpropanoid biosynthesis pathway, jasmonate biosynthesis pathway, and MYB transcription factors.

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Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.)

Cited by 35 publications

References 43 publications

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

A Sterility Induction Trait in the Genic Male Sterility Wheat Line 4110S Induced by High Temperature and its Cytological Response

Identification of Candidate Genes and Biosynthesis Pathways Related to Fertility Conversion by Wheat KTM3315A Transcriptome Profiling

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