Gene expression and biochemical responses in brain of zebrafish Danio rerio exposed to organic nanomaterials: Carbon nanotubes (SWCNT) and fullerenol (C60(OH)18–22(OK4))

Rocha, Ângela Sanches; Ferreira, Josencler Ribas; Barros, Daniela M.; Pereira, Talita Carneiro Brandão; Bogo, Maurı́cio Reis; Oliveira, Sabrynna Brito; Geraldo, Viviany; Lacerda, Rodrigo G.; Ferlauto, André S.; Ladeira, Luiz Orlando; Pinheiro, M. V. B.; Monserrat, José M.

doi:10.1016/j.cbpa.2013.03.025

Cited by 30 publications

(21 citation statements)

References 46 publications

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“…Intraperitoneal injections were conducted using a 3/10-mL U-100 BD ultra-fine short insulin syringe 8 mm (5/16′′) × 31G short veedle (Becton Dickinson and Company, NJ, USA) according to the protocol established by Phelps and colleagues [29]. Briefly, the volume injected into the animal (mean injection volume was 10 μ L) was adjusted to the fish bodyweight (mean mass of the animals was 0.75 g ± 0.06 g/mean ± SEM) to achieve 7.5, 15, and 30 mg/kg based on a previous study [30]. The animals of the control group received the same volume of saline solution, and the animals of the vehicle control received the same volume of 12.5% DMSO.…”

Section: Methodsmentioning

confidence: 99%

Intraperitoneal Exposure to Nano/Microparticles of Fullerene (C60) Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish (Danio rerio) Brain

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Kist

Azevedo

et al. 2013

BioMed Research International

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Even though technologies involving nano/microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biological properties that are important for the development of many technological applications. The aim of this study was to evaluate the consequences of nonphotoexcited fullerene C60 exposure in brain acetylcholinesterase expression and activity, antioxidant responses, and oxidative damage using adult zebrafish as an animal model. None of the doses tested (7.5, 15, and 30 mg/kg) altered AChE activity, antioxidant responses, and oxidative damage when zebrafish were exposed to nonphotoexcited C60 nano/microparticles during 6 and 12 hours. However, adult zebrafish exposed to the 30 mg/kg dose for 24 hours have shown enhanced AChE activity and augmented lipid peroxidation (TBARS assays) in brain. In addition, the up-regulation of brain AChE activity was neither related to the transcriptional control (RT-qPCR analysis) nor to the direct action of nonphotoexcited C60 nano/microparticles on the protein (in vitro results) but probably involved a posttranscriptional or posttranslational modulation of this enzymatic activity. Taken together these findings provided further evidence of toxic effects on brain after C60 exposure.

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Section: Methodsmentioning

confidence: 99%

Intraperitoneal Exposure to Nano/Microparticles of Fullerene (C60) Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish (Danio rerio) Brain

Forno

Kist

Azevedo

et al. 2013

BioMed Research International

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“…However, few studies reported the effects of CNT on the central nervous system of animals. Studies of systemically exposed mice have shown cognitive deficits and increased oxidative stress levels in brain [ 4 ], although these effects were not observed in fishes [ 5 , 6 ]. It should be considered that systemic administration of CNT likely results in different biodistribution and toxicity profiles based on the type of nanomaterial, the route of administration, and the animal model used [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

PEGylated Carbon Nanotubes Impair Retrieval of Contextual Fear Memory and Alter Oxidative Stress Parameters in the Rat Hippocampus

Bosco

Weber

Parfitt

et al. 2015

BioMed Research International

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Carbon nanotubes (CNT) are promising materials for biomedical applications, especially in the field of neuroscience; therefore, it is essential to evaluate the neurotoxicity of these nanomaterials. The present work assessed the effects of single-walled CNT functionalized with polyethylene glycol (SWCNT-PEG) on the consolidation and retrieval of contextual fear memory in rats and on oxidative stress parameters in the hippocampus. SWCNT-PEG were dispersed in water at concentrations of 0.5, 1.0, and 2.1 mg/mL and infused into the rat hippocampus. The infusion was completed immediately after training and 30 min before testing of a contextual fear conditioning task, resulting in exposure times of 24 h and 30 min, respectively. The results showed that a short exposure to SWCNT-PEG impaired fear memory retrieval and caused lipid peroxidation in the hippocampus. This response was transient and overcome by the mobilization of antioxidant defenses at 24 h. These effects occurred at low and intermediate but not high concentration of SWCNT-PEG, suggesting that the observed biological response may be related to the concentration-dependent increase in particle size in SWCNT-PEG dispersions.

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“…The increased expression of Nrf2 was also reported in the brains of zebrafish after systemic exposure to SWCNT [46]. These studies demonstrated the ability of carbon nanomaterials to induce antioxidant defenses and to prevent a potential damage to the neural tissue.…”

Section: Resultsmentioning

confidence: 57%

Biopersistence of PEGylated Carbon Nanotubes Promotes a Delayed Antioxidant Response after Infusion into the Rat Hippocampus

et al. 2015

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Carbon nanotubes are promising nanomaterials for the diagnosis and treatment of brain disorders. However, the ability of these nanomaterials to cross cell membranes and interact with neural cells brings the need for the assessment of their potential adverse effects on the nervous system. This study aimed to investigate the biopersistence of single-walled carbon nanotubes functionalized with polyethylene glycol (SWCNT-PEG) directly infused into the rat hippocampus. Contextual fear conditioning, Y-maze and open field tasks were performed to evaluate the effects of SWCNT-PEG on memory and locomotor activity. The effects of SWCNT-PEG on oxidative stress and morphology of the hippocampus were assessed 1 and 7 days after infusion of the dispersions at 0.5, 1.0 and 2.1 mg/mL. Raman analysis of the hippocampal homogenates indicates the biopersistence of SWCNT-PEG in the hippocampus 7 days post-injection. The infusion of the dispersions had no effect on the acquisition or persistence of the contextual fear memory; likewise, the spatial recognition memory and locomotor activity were not affected by SWCNT-PEG. Histological examination revealed no remarkable morphological alterations after nanomaterial exposure. One day after the infusion, SWCNT-PEG dispersions at 0.5 and 1.0 mg/mL were able to decrease total antioxidant capacity without modifying the levels of reactive oxygen species or lipid hydroperoxides in the hippocampus. Moreover, SWCNT-PEG dispersions at all concentrations induced antioxidant defenses and reduced reactive oxygen species production in the hippocampus at 7 days post-injection. In this work, we found a time-dependent change in antioxidant defenses after the exposure to SWCNT-PEG. We hypothesized that the persistence of the nanomaterial in the tissue can induce an antioxidant response that might have provided resistance to an initial insult. Such antioxidant delayed response may constitute an adaptive response to the biopersistence of SWCNT-PEG in the hippocampus.

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Gene expression and biochemical responses in brain of zebrafish Danio rerio exposed to organic nanomaterials: Carbon nanotubes (SWCNT) and fullerenol (C60(OH)18–22(OK4))

Cited by 30 publications

References 46 publications

Intraperitoneal Exposure to Nano/Microparticles of Fullerene (C60) Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish (Danio rerio) Brain

Intraperitoneal Exposure to Nano/Microparticles of Fullerene (C60) Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish (Danio rerio) Brain

PEGylated Carbon Nanotubes Impair Retrieval of Contextual Fear Memory and Alter Oxidative Stress Parameters in the Rat Hippocampus

Biopersistence of PEGylated Carbon Nanotubes Promotes a Delayed Antioxidant Response after Infusion into the Rat Hippocampus

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