Gene expression analysis of wounding‐induced root‐to‐shoot communication in <i>Arabidopsis thaliana</i>

Hasegawa, Satoko; Sogabe, Yusuke; Asano, Taku; Nakagawa, Tomoyuki; Nakamura, Haruna; Kodama, Hiroaki; Ohta, Hiroyuki; Yamaguchi, Kazuo; Mueller, Martin J.; Nishiuchi, Takumi

doi:10.1111/j.1365-3040.2011.02274.x

Cited by 33 publications

(39 citation statements)

References 64 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, wounding or systemin treatment of tomato roots caused induction of systemic responses in leaves and this was dependent on JA signaling (Li et al, 2002), and wounding of Arabidopsis roots caused increased JA biosynthesis in shoots but not roots (Hasegawa et al, 2011). Thus it is possible that LBD20 may be involved in the development of regulatory signals that move from roots to shoots.…”

Section: Discussionmentioning

confidence: 99%

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) gene family encodes plant-specific transcriptional regulators functioning in organ development. In a screen of Arabidopsis (Arabidopsis thaliana) sequence-indexed transferred DNA insertion mutants, we found disruption of the LOB DOMAIN-CONTAINING PROTEIN20 (LBD20) gene led to increased resistance to the root-infecting vascular wilt pathogen Fusarium oxysporum. In wild-type plants, LBD20 transcripts were barely detectable in leaves but abundant in roots, where they were further induced after F. oxysporum inoculation or methyl jasmonate treatment. Induction of LBD20 expression in roots was abolished in coronatine insensitive1 (coi1) and myc2 (allelic to jasmonate insensitive1) mutants, suggesting LBD20 may function in jasmonate (JA) signaling. Consistent with this, expression of the JA-regulated THIONIN2.1 (Thi2.1) and VEGETATIVE STORAGE PROTEIN2 (VSP2) genes were up-regulated in shoots of lbd20 following treatment of roots with F. oxysporum or methyl jasmonate. However, PLANT DEFENSIN1.2 expression was unaltered, indicating a repressor role for LBD20 in a branch of the JA-signaling pathway. Plants overexpressing LBD20 (LBD20-OX) had reduced Thi2.1 and VSP2 expression. There was a significant correlation between increased LBD20 expression in the LBD20-OX lines with both Thi2.1 and VSP2 repression, and reduced survival following F. oxysporum infection. Chlorosis resulting from application of F. oxysporum culture filtrate was also reduced in lbd20 leaves relative to the wild type. Taken together, LBD20 is a F. oxysporum susceptibility gene that appears to regulate components of JA signaling downstream of COI1 and MYC2 that are required for full elicitation of F. oxysporum-and JA-dependent responses. To our knowledge, this is the first demonstration of a role for a LBD gene family member in either biotic stress or JA signaling.

show abstract

Section: Discussionmentioning

confidence: 99%

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…In addition, ethylene (ET)-related genes such as 1-aminocyclopropane-1-carboxylic acid (ACC) synthase 6 (ACS6 ) were also induced in the shoots with wounded roots. 6,8 In fact, root-to-shoot response of some early RtS genes examined in the response. For this purpose, we introduced some RtS promoter::firefly Luc fusion genes into Arabidopsis plants.…”

Section: Ja and Opda Regulate The Wound-induced Root-to-shoot Responsmentioning

confidence: 99%

“…Therefore, we tried to establish a model system to monitor the gene expression during wound-induced root-to-shoot communication in Arabidopsis seedlings. 6 Then, we have performed the transcriptome analysis of wound-induced root-toshoot response in Arabidopsis. 6 When the roots of Arabidopsis seedlings were wounded, the expression in the shoots of many genes was upregulated more than three-fold at 30 min and 6 h post-injury.…”

mentioning

confidence: 99%

“…6 Then, we have performed the transcriptome analysis of wound-induced root-toshoot response in Arabidopsis. 6 When the roots of Arabidopsis seedlings were wounded, the expression in the shoots of many genes was upregulated more than three-fold at 30 min and 6 h post-injury. 6 …”

mentioning

confidence: 99%

“…6 When the roots of Arabidopsis seedlings were wounded, the expression in the shoots of many genes was upregulated more than three-fold at 30 min and 6 h post-injury. 6 …”

mentioning

confidence: 99%

See 2 more Smart Citations

Visualization of wounding-induced root-to-shoot communication in arabidopsis

Sogabe

Nakamura

Nakagawa

et al. 2011

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

It is known that wounding systemically activates the expression of various defense-related genes in plants. However, most studies of wound-induced systemic response are concerned with a leaf-toleaf response. We have recently reported that the long distance signaling was also observed in the shoots of Arabidopsis seedling with wounded roots. We identified early and late root-to-shoot responsive (RtS) genes that were upregulated in the shoots of root-wounded seedlings at 30 min and 6 h post-injury, respectively. It is likely that the primary signals were rapidly transfered from injured roots to shoots, and then these signals were converted into chemical signals. In fact, increase of JA and OPDA content activated the expression of early and late RtS genes in shoots, respectively. In addition, we visualized wound-induced root-to-shoot response by using RtS promoter-luciferase (Luc) transgenic plants. Analysis of the AtERF13 promoter::Luc transgenic plants clearly shows that the wound-induced root-to-shoot signaling was rapidly activated via the vascular systems.In higher plants, "root-to-hoot" signaling plays an important role in their adaptation in response to various environmental stresses such as dehydration, flooding, larvae attack and pathogen infection.

show abstract

Comparative transcriptome profiling reveals distinct regulatory responses of secondary defensive metabolism in Datura species (Solanaceae) under plant development and herbivory‐mediated stress

Kariñho Betancourt,

Calderón Cortés,

Tapia López

et al. 2024

Ecology and Evolution

View full text Add to dashboard Cite

Differential expression of genes is key to mediating developmental and stress‐related plant responses. Here, we addressed the regulation of plant metabolic responses to biotic stress and the developmental variation of defense‐related genes in four species of the genus Datura with variable patterns of metabolite accumulation and development. We combine transcriptome profiling with phylogenomic techniques to analyze gene expression and coexpression in plants subjected to damage by a specialist folivore insect. We found (1) common overall gene expression in species of similar chemical profiles, (2) species‐specific responses of proteins involved in specialized metabolism, characterized by constant levels of gene expression coupled with transcriptional rearrangement, and (3) induction of transcriptional rearrangement of major terpene and tropane alkaloid genes upon herbivory. Our results indicate differential modulation of terpene and tropane metabolism linked to jasmonate signaling and specific transcription factors to regulate developmental variation and stress programs, and suggest plastic adaptive responses to cope with herbivory. The transcriptional profiles of specialized metabolism shown here reveal complex genetic control of plant metabolism and contribute to understanding the molecular basis of adaptations and the physiological variation of significant ecological traits.

show abstract

Gene expression analysis of wounding‐induced root‐to‐shoot communication in Arabidopsis thaliana

Cited by 33 publications

References 64 publications

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

Visualization of wounding-induced root-to-shoot communication in arabidopsis

Comparative transcriptome profiling reveals distinct regulatory responses of secondary defensive metabolism in Datura species (Solanaceae) under plant development and herbivory‐mediated stress

Contact Info

Product

Resources

About