Gene Expression Analysis of Microtubers of Potato Solanum tuberosum L. Induced in Cytokinin Containing Medium and Osmotic Stress

Abstract

Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using osmotic stress and cytokinin signaling will help us to elucidate this process. We demonstrate in this work a rapid and efficient protocol for microtuber development and gene expression analysis. Medium with high content of sucrose and gelrite supplemented with 2iP as cytokinin under darkness condition produced the higher quantity and quality of microtubers. Gene expression analysis of genes involved in the two-component signaling system (StHK1), cytokinin signaling, (StHK3, StHP4, StRR1) homeodomains (WUSCHEL, POTH1, BEL5), auxin signaling, ARF5, carbon metabolism (TPI, TIM), protein synthesis, NAC5 and a morphogenetic regulator of tuberization (POTH15) was performed by qPCR real time. Differential gene expression was observed during microtuber development. Gene regulation of two component and cytokinin signaling is taking place during this developmental process, yielding more microtubers. Further analysis of each component is required to elucidate it.

In Vitro Propagation and Phytochemistry of Thymol-Producing Plants from a Horticultural Form of Thymus × josephi-angeli Mansanet & Aguil. (Lamiaceae)

“…However, particularities in the different cytokinin perception by the plant cells and signal transduction at the molecular level by a differential genetic expression might be associated with a wide array of factors. For instance, Herrera-Isidron et al [47] recently proposed differential cytokinin perception depending on certain developmental events during the organogenesis in potatoes. In addition, it is still not clear whether cytokinin signaling takes place from one or more than one site at the cellular level (endoplasmic reticulum and/or plasma membrane), if the action is equal in all tissues or if it depends on the tissue, its developmental stage, and/or the action of signalling molecules from different origins [48], or if synergies exist between endogenous levels of hormones and exogenously applied PGRs [49].…”

Exploring the Potential Role of Ribosomal Proteins to Enhance Potato Resilience in the Face of Changing Climatic Conditions

“…In the control medium, MS medium was supplemented with 1% sucrose, 3 g/L gelrite and 10 mg/L 2iP identified as MR1-G3-2iP. Flasks were sealed with plastic and incubated in the dark at 25/17 • C for 15 days [6].…”

“…In our previous work, we developed a protocol for MTs induction in potato S. tuberosum var α using stolons cultured in MS medium supplemented with 8% sucrose, gelrite 6.0 g/L, 2iP 10 mg/L under darkness conditions. The rationale for this protocol was that CK signaling interacts with homeobox transcription factors, RPs, cell cycle, carbon metabolism, auxin-responsive factors and stem cell genes in the microtuberization process [6].…”

Effect of moderate heat stress on tuberization of potato genotypes: Morphological and physiological changes using in vitro and ex vitro propagation

“…In recent years, studies on the influence of various physiological, genetic and microbiological factors on potato tuberization have been diverse. Thus, we have the studies on hormonal regulation and auxincytokinin signaling pathways in gene expression (Kolachevskaya et al, 2021), the use of supplements (Dalimunthe et al, 2021), the effect of red LED light and blue LED light, correlated with a greater expression of tuberization-related genes (Pundir et al, 2021), the use of a culture medium with a high content of sucrose and gelrite (Herrera-Isidron et al, 2021), and the application of microbiological substances (Fedorova et al, 2021). However, there are not many studies that relate potato tuberization to stress conditions, especially heat stress, hence the need for this study to fill a gap in our current knowledge.…”