Gene expression analysis of mammary tissue during fetal bud formation and growth in two pig breeds – indications of prenatal initiation of postnatal phenotypic differences

Chomwisarutkun, K.; Muráni, Eduard; Ponsuksili, Siriluck; Wimmers, Klaus

doi:10.1186/1471-213x-12-13

Cited by 6 publications

(9 citation statements)

References 53 publications

(38 reference statements)

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“…In multiparous pigs, the number and the shape of functional mammary gland complexes are major determinants of the mothering ability of sows. Sow milk yield and quality is crucial for the survival and growth of piglets [ 16 ]. The mammary gland prepares for lactation by undergoing extensive growth and differentiation during pregnancy.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Profiling of Lactating Mammary Gland in Divergent Phenotype Swine Breeds

Peng

Zhao

Shen

et al. 2015

IJMS

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MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

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Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Profiling of Lactating Mammary Gland in Divergent Phenotype Swine Breeds

Peng

Zhao

Shen

et al. 2015

IJMS

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“…hNSSCs were isolated and cultured in accordance with our previously published protocols [ 6 , 8 ]. In brief, cells were isolated by explant organ culture to establish outgrowth cell culture ( Figure 1(a) ).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, hNSSCs were shown to express thirty-three CD markers including known stromal cell-associated as well as several novel markers [ 6 ]. Moreover, these cells could be induced to differentiate into cells expressing endothelial markers and to form densely packed large diameter tubules during in vitro angiogenesis assay [ 5 , 8 ]. However, the angiogenic capacity of hNSSCs ex vivo remains unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, there is substantial heterogeneity in the system of recruitment, collection, and storage of autologous clinical grade source [ 9 ]. Our preliminary studies using neonatal foreskin showed promising results indicating that hNSSCs could be an alternative potential source for cell based angiogenesis [ 6 , 8 ]. Thus, improved understanding of the cellular mechanisms of hNSSCs vasculogenesis and angiogenesis could offer new therapeutic approaches for hNSSCs.…”

Section: Introductionmentioning

confidence: 99%

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Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model

Vishnubalaji

Atteya

Al-Nbaheen

et al. 2015

Stem Cells International

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Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

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“…Moreover, RAPGEF1 was also found to be a differentially expressed gene relevant to mammogenesis in pigs during fetal bud formation (Chomwisarutkun et al . ). Thus, we inferred that the SSC5:60296617 SNP may affect teat number by regulating the interaction of EPS8 and RAPGEF1 and, finally, affecting the mammogenesis of pigs.…”

Section: Effect Of the Ssc5:60296617 Snp On Teat Number Traitsmentioning

confidence: 97%

Genotyping by sequencing reveals a new locus for pig teat number

Wang

Zhang

et al. 2017

Animal Genetics

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Teat number is an extremely valuable trait for sow reproduction performance and piglet survival. Here, we used genotyping-by-sequencing and a general liner model to carry out genome-wide analysis of sow total teat number in an Erhualian population. The results indicated that eight SNPs on chromosomes 4, 5, 9 and 10 were significant genome wide (Bonferroni method, P < 2.85E-7) for sow total teat number. Validation analyses were performed in 298 Erhualian and 904 Large White sows using these significant SNPs and the general liner model procedure in sas. Finally, only the SNP on chromosome 5 was found to be significantly associated with sow teat number in both populations. The GG genotype individuals had 2.23 and 0.82 more teat numbers respectively than did the AA genotype individuals in these two populations (P < 0.05). According to the expression and annotation analyses, we inferred the presence of a gene or lincRNA that could affect teat number by regulating other genes and ultimately affecting the mammogenesis of pigs. Further studies using methods such as Cas9 editing and gene silencing analysis are necessary for additional function analysis.

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Gene expression analysis of mammary tissue during fetal bud formation and growth in two pig breeds – indications of prenatal initiation of postnatal phenotypic differences

Cited by 6 publications

References 53 publications

MicroRNA Expression Profiling of Lactating Mammary Gland in Divergent Phenotype Swine Breeds

MicroRNA Expression Profiling of Lactating Mammary Gland in Divergent Phenotype Swine Breeds

Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model

Genotyping by sequencing reveals a new locus for pig teat number

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