Gene expression analysis of human red blood cells

Kabanova, Sveta; Kleinbongard, Petra; Volkmer, Jens Peter; Andrée, Birgit; Kelm, Malte; Jax, Thomas

doi:10.7150/ijms.6.156

Cited by 90 publications

(67 citation statements)

References 19 publications

(22 reference statements)

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“…Evidence has recently been accumulating of the ability of adult human RBCs to secrete signalling entities or transmitter molecules, including nucleotides, gases and other mediators that take part in cell-cell communications and control biochemical processes. Transcripts of 1019 different genes involved in cellular metabolism, signal transduction, development, immune responses, programmed cell death and autophagy have been identified in circulating human RBCs (Kabanova et al, 2009;CorteseKrott & Kelm, 2014).…”

Section: Discussionmentioning

confidence: 99%

Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates

et al. 2016

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SummaryPregnancy is a state associated with an enhanced metabolism and demand for O 2 , which may lead to the overproduction of reactive oxygen species (ROS) and hence to oxidative stress. An elevated ROS level may result in delayed development and a low birth weight. The aim of this study was to reveal the consequences of multiple pregnancies on the redox status of neonatal human red blood cells (RBCs) and evaluate the role of endothelial nitric oxide synthase (NOS3) -expressing RBCs in the generation of oxidative stress. The study presents evidence of higher levels of production of hydrogen peroxide, peroxynitrite and nitrate content in the RBCs of twin neonates, clearly reflected by an elevated level of protein and lipid damages. This phenotype appears to be a consequence of multiple pregnancies, regardless of the level of maturity or the birth weight of the twins. Besides the higher level of ROS, there was a general decrease in the expression of genes coding for antioxidants. The first data are presented on NOS3-expressing neonatal human RBCs. The number of RBCs producing NOS3 was more than twice as high in twin neonates compared to singletons, with no correlation to maturity.

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Section: Discussionmentioning

confidence: 99%

Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates

et al. 2016

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“…Red blood cells contain no nucleus, and protein biosynthesis is assumed to be absent in these cells. However, mRNAs for the translational machinery are still detected in mature red blood cells (35). Furthermore, many Plasmodium species preferentially invade reticulocytes (36), which contain high levels of tRNAs (34) compared with mature erythrocytes (35).…”

Section: Discussionmentioning

confidence: 99%

Apicomplexa -specific tRip facilitates import of exogenous tRNAs into malaria parasites

Bour

Mahmoudi

Kapps

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The malaria-causing Plasmodium parasites are transmitted to vertebrates by mosquitoes. To support their growth and replication, these intracellular parasites, which belong to the phylum Apicomplexa, have developed mechanisms to exploit their hosts. These mechanisms include expropriation of small metabolites from infected host cells, such as purine nucleotides and amino acids. Heretofore, no evidence suggested that transfer RNAs (tRNAs) could also be exploited. We identified an unusual gene in Apicomplexa with a coding sequence for membrane-docking and structurespecific tRNA binding. This Apicomplexa protein-designated tRip (tRNA import protein)-is anchored to the parasite plasma membrane and directs import of exogenous tRNAs. In the absence of tRip, the fitness of the parasite stage that multiplies in the blood is significantly reduced, indicating that the parasite may need host tRNAs to sustain its own translation and/or as regulatory RNAs. Plasmodium is thus the first example, to our knowledge, of a cell importing exogenous tRNAs, suggesting a remarkable adaptation of this parasite to extend its reach into host cell biology.tRNA | Plasmodium | trafficking

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“…Mature human erythrocytes, the main cellular components of blood, are anucleate cells; thus they are not capable of active RNA synthesis. However, Kabanova et al (2009) provided strong evidence that red blood cells have substantial RNA content. This fact supports the assumption of nucleus-independent protein synthesis and an obvious lack of the transcriptional regulation of gene expression in mature erythrocytes.…”

Section: P450 Expression In Leukocytesmentioning

confidence: 99%

Estimation of Drug-Metabolizing Capacity by Cytochrome P450 Genotyping and Expression

Temesvári

Kopper

Paulik³

et al. 2012

J Pharmacol Exp Ther

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Many undesired side effects or therapeutic failures of drugs are the result of differences or changes in drug metabolism, primarily depending on the levels and activities of cytochrome P450 (P450) enzymes. To assess whether P450 expression profiles can reflect hepatic drug metabolism, we compared P450 mRNA levels in the liver or peripheral leukocytes with the corresponding hepatic P450 activities. A preliminary P450 genotyping for the most frequent polymorphisms in white populations (CYP2C9*2, CYP2C9*3, CYP2C19*2, CYP2C19*3, CYP2D6*3, CYP2D6*4, CYP2D6*6, and CYP3A5*3) was carried out before P450 phenotyping, excluding the donors with nonfunctional alleles of CYP2C9, CYP2C19, and CYP2D6 and those with a functional CYP3A5*1 allele from a correlation analysis. The hepatic mRNA levels of CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 displayed a strong association with P450 activities in the liver, whereas the expression of CYP1A2, CYP2C9, CYP2C19, and CYP3A4 in leukocytes was proven to reflect the hepatic activities of these P450 species. The leukocytes were found to be inappropriate cells for the assessment of hepatic CYP2B6 and CYP2D6 activities. Combining the results of P450 genotyping and phenotyping analyses, patients' drug-metabolizing capacities can be estimated by the P450 expression in the liver and in leukocytes with some limitations. Patients' genetic and nongenetic variations in P450 status can guide the appropriate selection of drugs and the optimal dose, minimizing the risk of harmful side effects and ensuring a successful outcome of drug therapy.

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Gene expression analysis of human red blood cells

Cited by 90 publications

References 19 publications

Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates

Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates

Apicomplexa -specific tRip facilitates import of exogenous tRNAs into malaria parasites

Estimation of Drug-Metabolizing Capacity by Cytochrome P450 Genotyping and Expression

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