Gene expression analysis of four WIR1-like genes in floret tissues of European winter wheat after challenge with G. zeae

Diethelm, M.; Rhiel, M.; Wagner, Christian; Mikolajewski, Sabine; Groth, Jennifer; Hartl, L.; Friedt, Wolfgang; Schweizer, Günther

doi:10.1007/s10681-011-0498-7

Cited by 7 publications

(8 citation statements)

References 52 publications

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“…Previous transcriptomic studies on the F. graminearum –wheat interaction have led to the characterization of multiple defence‐associated gene classes that describe a general or genotype‐specific response of wheat to F. graminearum or DON (Ansari et al ., ; Bernardo et al ., ; Diethelm et al ., ; Ding et al ., ; Foroud et al ., ; Golkari et al ., ; Gottwald et al ., ; Steiner et al ., ; Walter et al ., ). Walter et al .…”

Section: Discussionmentioning

confidence: 99%

“…Most of the F. graminearum-responsive genes found represent a general response to the pathogen, regardless of resistance level and QTL. Previous transcriptomic studies on the F. graminearum-wheat interaction have led to the characterization of multiple defenceassociated gene classes that describe a general or genotype- specific response of wheat to F. graminearum or DON (Ansari et al, 2007;Bernardo et al, 2007;Diethelm et al, 2012;Ding et al, 2011;Foroud et al, 2011;Golkari et al, 2009;Gottwald et al, 2012;Steiner et al, 2009;Walter et al, 2008). Walter et al (2008) examined the gene expression of DONtreated doubled haploid (DH) lines segregating for Fhb1, and reported the Fhb1-dependent upregulation of several genes.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa‐5A, identifies novel candidate genes

Schweiger

Steiner

Ametz

et al. 2013

Molecular Plant Pathology

111

105

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Fusarium head blight, caused by Fusarium graminearum, is a devastating disease of wheat. We developed near-isogenic lines (NILs) differing in the two strongest known F. graminearum resistance quantitative trait loci (QTLs), Qfhs.ndsu-3BS (also known as resistance gene Fhb1) and Qfhs.ifa-5A, which are located on the short arm of chromosome 3B and on chromosome 5A, respectively. These NILs showing different levels of resistance were used to identify transcripts that are changed significantly in a QTL-specific manner in response to the pathogen and between mock-inoculated samples. After inoculation with F. graminearum spores, 16 transcripts showed a significantly different response for Fhb1 and 352 for Qfhs.ifa-5A. Notably, we identified a lipid transfer protein which is constitutively at least 50-fold more abundant in plants carrying the resistant allele of Qfhs.ifa-5A. In addition to this candidate gene associated with Qfhs.ifa-5A, we identified a uridine diphosphate (UDP)-glycosyltransferase gene, designated TaUGT12887, exhibiting a positive difference in response to the pathogen in lines harbouring both QTLs relative to lines carrying only the Qfhs.ifa-5A resistance allele, suggesting Fhb1 dependence of this transcript. Yet, this dependence was observed only in the NIL with already higher basal resistance. The complete cDNA of TaUGT12887 was reconstituted from available wheat genomic sequences, and a synthetic recoded gene was expressed in a toxin-sensitive strain of Saccharomyces cerevisiae. This gene conferred deoxynivalenol resistance, albeit much weaker than that observed with the previously characterized barley HvUGT13248.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa‐5A, identifies novel candidate genes

Schweiger

Steiner

Ametz

et al. 2013

Molecular Plant Pathology

111

105

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“…This result is in agreement with the work of Long et al ( 2015 ) where a member of the WIR1 family, WIR1A, was more expressed in the R 2DL+ 2-2618 spikelets than in the S null 2-2890 spikelets during F. graminearum infection. Although WIR1A has not been functionally characterized, it is thought to play a role in basal and non-host resistance and two WIR1A variants were mapped within the interval of a wheat genomic locus on chromosome 7BS that confers FHB resistance (Diethelm et al, 2012 ). Thus, the high level of miR9653a identified in the S null 2-2890 and the higher expression of WIR1A (and other members of the WIR1 family) in the R NILS might contribute to the differential response to FHB recorded among the two NILs.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Profiles of Near-Isogenic Hexaploid Wheat Lines Differing for Effective Alleles at the 2DL FHB Resistance QTL

Biselli¹,

Bagnaresi²,

Faccioli³

et al. 2018

Front. Plant Sci.

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Fusarium head blight (FHB), caused by the fungus Fusarium graminearum, represents one of the major wheat diseases worldwide, determining severe yield losses and reduction of grain quality due to the accumulation of mycotoxins. The molecular response associated with the wheat 2DL FHB resistance QTL was mined through a comprehensive transcriptomic analysis of the early response to F. graminearum infection, at 3 days post-inoculation, in spikelets and rachis. The analyses were conducted on two near isogenic lines (NILs) differing for the presence of the 2DL QTL (2-2618, resistant 2DL+ and 2-2890, susceptible null). The general response to fungal infection in terms of mRNAs accumulation trend was similar in both NILs, even though involving an higher number of DEGs in the susceptible NIL, and included down-regulation of the primary and energy metabolism, up-regulation of enzymes implicated in lignin and phenylpropanoid biosynthesis, activation of hormons biosynthesis and signal transduction pathways and genes involved in redox homeostasis and transcriptional regulation. The search for candidate genes with expression profiles associated with the 2DL QTL for FHB resistance led to the discovery of processes differentially modulated in the R and S NILs related to cell wall metabolism, sugar and JA signaling, signal reception and transduction, regulation of the redox status and transcription factors. Wheat FHB response-related miRNAs differentially regulated were also identified as putatively implicated in the superoxide dismutase activities and affecting genes regulating responses to biotic/abiotic stresses and auxin signaling. Altered gene expression was also observed for fungal non-codingRNAs. The putative targets of two of these were represented by the wheat gene WIR1A, involved in resistance response, and a gene encoding a jacalin-related lectin protein, which participate in biotic and abiotic stress response, supporting the presence of a cross-talk between the plant and the fungus.

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“…Therefore we do not expect significant differences in the recorded metabolomics and transcriptomics data sets (an example for phenylalanine and is given in Figure S15 ). Comparable transcriptomics and metabolomics studies have used a wide range of concentrations from 200 to 1000 conidia/floret to elicit a defense response to FHB ( Jia et al 2009 ; Gottwald et al 2012 ; Foroud et al 2012 ; Zhuang et al 2013 ; Schweiger et al 2013 ; Steiner et al 2009 ; Gunnaiah et al 2012 ; Diethelm et al 2012 ). However, we cannot fully exclude the possibility that the observed metabolite changes, i.e.…”

Section: Discussionmentioning

confidence: 99%

Joint Transcriptomic and Metabolomic Analyses Reveal Changes in the Primary Metabolism and Imbalances in the Subgenome Orchestration in the Bread Wheat Molecular Response toFusarium graminearum

Nußbaumer

Warth

Sharma

et al. 2015

G3 Genes|Genomes|Genetics

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Fusarium head blight is a prevalent disease of bread wheat (Triticum aestivum L.), which leads to considerable losses in yield and quality. Quantitative resistance to the causative fungus Fusarium graminearum is poorly understood. We integrated transcriptomics and metabolomics data to dissect the molecular response to the fungus and its main virulence factor, the toxin deoxynivalenol in near-isogenic lines segregating for two resistance quantitative trait loci, Fhb1 and Qfhs.ifa-5A. The data sets portrait rearrangements in the primary metabolism and the translational machinery to counter the fungus and the effects of the toxin and highlight distinct changes in the metabolism of glutamate in lines carrying Qfhs.ifa-5A. These observations are possibly due to the activity of two amino acid permeases located in the quantitative trait locus confidence interval, which may contribute to increased pathogen endurance. Mapping to the highly resolved region of Fhb1 reduced the list of candidates to few genes that are specifically expressed in presence of the quantitative trait loci and in response to the pathogen, which include a receptor-like protein kinase, a protein kinase, and an E3 ubiquitin-protein ligase. On a genome-scale level, the individual subgenomes of hexaploid wheat contribute differentially to defense. In particular, the D subgenome exhibited a pronounced response to the pathogen and contributed significantly to the overall defense response.

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Gene expression analysis of four WIR1-like genes in floret tissues of European winter wheat after challenge with G. zeae

Abstract: Fusarium head blight (FHB) is a highly destructive disease of wheat and other cereals which causes serious mycotoxin contaminations of grain.

Cited by 7 publications

References 52 publications

Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa‐5A, identifies novel candidate genes

Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa‐5A, identifies novel candidate genes

Comparative Transcriptome Profiles of Near-Isogenic Hexaploid Wheat Lines Differing for Effective Alleles at the 2DL FHB Resistance QTL

Joint Transcriptomic and Metabolomic Analyses Reveal Changes in the Primary Metabolism and Imbalances in the Subgenome Orchestration in the Bread Wheat Molecular Response toFusarium graminearum

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