Gene expression analyses in malformed skeletal structures of gilthead sea bream (<i>Sparus aurata</i>)

Riera‐Heredia, Natàlia; Vélez, Emilio J.; Gutiérrez, Joaquím; Navarro, Isabel; Capilla, Encarnación

doi:10.1111/jfd.13019

Cited by 12 publications

(9 citation statements)

References 62 publications

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“…In gilthead sea bream, osteocalcin appearance at around 30 days post-hatching coincides with the onset of skeleton mineralization ( 245 ), and the levels of expression at that time are greatly induced in response to increased incubation temperature during embryogenesis ( 262 ), which can be related with accelerated growth and the appearance of skeletal abnormalities. Indeed, inverse correlation between malformation severity and osteocalcin levels have been reported in fish ( 263 – 265 ). These evidences are supported by the knockout mice study in which osteocalcin deficiency led to higher bone mass by increased and accelerated bone formation ( 266 ).…”

Section: Osteokinesmentioning

confidence: 98%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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Control of tissue metabolism and growth involves interactions between organs, tissues, and cell types, mediated by cytokines or direct communication through cellular exchanges. Indeed, over the past decades, many peptides produced by adipose tissue, skeletal muscle and bone named adipokines, myokines and osteokines respectively, have been identified in mammals playing key roles in organ/tissue development and function. Some of them are released into the circulation acting as classical hormones, but they can also act locally showing autocrine/paracrine effects. In recent years, some of these cytokines have been identified in fish models of biomedical or agronomic interest. In this review, we will present their state of the art focusing on local actions and inter-tissue effects. Adipokines reported in fish adipocytes include adiponectin and leptin among others. We will focus on their structure characteristics, gene expression, receptors, and effects, in the adipose tissue itself, mainly regulating cell differentiation and metabolism, but in muscle and bone as target tissues too. Moreover, lipid metabolites, named lipokines, can also act as signaling molecules regulating metabolic homeostasis. Regarding myokines, the best documented in fish are myostatin and the insulin-like growth factors. This review summarizes their characteristics at a molecular level, and describes both, autocrine effects and interactions with adipose tissue and bone. Nonetheless, our understanding of the functions and mechanisms of action of many of these cytokines is still largely incomplete in fish, especially concerning osteokines (i.e., osteocalcin), whose potential cross talking roles remain to be elucidated. Furthermore, by using selective breeding or genetic tools, the formation of a specific tissue can be altered, highlighting the consequences on other tissues, and allowing the identification of communication signals. The specific effects of identified cytokines validated through in vitro models or in vivo trials will be described. Moreover, future scientific fronts (i.e., exosomes) and tools (i.e., co-cultures, organoids) for a better understanding of inter-organ crosstalk in fish will also be presented. As a final consideration, further identification of molecules involved in inter-tissue communication will open new avenues of knowledge in the control of fish homeostasis, as well as possible strategies to be applied in aquaculture or biomedicine.

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Section: Osteokinesmentioning

confidence: 98%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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“…In this regeneration study, a regulatory role for myostatin could also be hypothesized, since bone decreased mstn2 expression by day 4 in response to the muscle injury possibly to allow proper muscle remodeling, thus giving more insights of the importance of myostatin between the bone and muscle crosstalk in fish. Finally, the ECM marker on showed a progressive increase during regeneration with the maximum expression reached at day 30 coinciding with the highest expression observed for on at the end of in vitro and in vivo osteogenesis in gilthead sea bream according to its key role regulating the later stages of tissue development ( 33 , 92 ). In summary, these results open a promising new line of research to study the possible crosstalk between bone and muscle in fish to regulate skeletal muscle development and growth.…”

Section: Discussionmentioning

confidence: 86%

“…The bmp2 and ctsk genes showed an early activation of expression at days 1 and 2 post-injury, respectively, although bmp2 expression decreased at day 8 recovering basal levels at the end of the experiment. This early upregulation of bmp2 could be related to the highest expression found for this molecule at the beginning of embryonic development in gilthead sea bream ( 92 ), but also to situations in which bone (and muscle) growth is stimulated, such as for example in Atlantic salmon ( Salmo salar ) reared at elevated water temperatures ( 93 ). Moreover, the peak of expression of ctsk at day 2 resembled the increase in expression observed after 20 days of fasting ( 57 ) suggesting that ctsk and therefore, bone resorption, may have a role in muscle remodeling after stressful conditions.…”

Section: Discussionmentioning

confidence: 99%

Muscle regeneration in gilthead sea bream: Implications of endocrine and local regulatory factors and the crosstalk with bone

et al. 2023

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Fish muscle regeneration is still a poorly known process. In the present study, an injury was done into the left anterior epaxial skeletal muscle of seventy 15 g gilthead sea bream (Sparus aurata) juveniles to evaluate at days 0, 1, 2, 4, 8, 16 and 30 post-wound, the expression of several muscle genes. Moreover, transcripts’ expression in the bone (uninjured tissue) was also analyzed. Histology of the muscle showed the presence of dead tissue the first day after injury and how the damaged fibers were removed and replaced by new muscle fibers by day 16 that kept growing up to day 30. Gene expression results showed in muscle an early upregulation of igf-2 and a downregulation of ghr-1 and igf-1. Proteolytic systems expression increased with capn2 and ctsl peaking at 1 and 2 days post-injury, respectively and mafbx at day 8. A pattern of expression that fitted well with active myogenesis progression 16 days after the injury was then observed, with the recovery of igf-1, pax7, cmet, and cav1 expression; and later on, that of cav3 as well. Furthermore, the first days post-injury, the cytokines il-6 and il-15 were also upregulated confirming the tissue inflammation, while tnfα was only upregulated at days 16 and 30 to induce satellite cells recruitment; overall suggesting a possible role for these molecules as myokines. The results of the bone transcripts showed an upregulation first, of bmp2 and ctsk at days 1 and 2, respectively; then, ogn1 and ocn peaked at day 4 in parallel to mstn2 downregulation, and runx2 and ogn2 increased after 8 days of muscle injury, suggesting a possible tissue crosstalk during the regenerative process. Overall, the present model allows studying the sequential involvement of different regulatory molecules during muscle regeneration, as well as the potential relationship between muscle and other tissues such as bone to control musculoskeletal development and growth, pointing out an interesting new line of research in this group of vertebrates.

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“…Malformations are congenital in origin, occurring early in development, and deformities occur on previously formed structures. Malformations can be caused by a variety of factors in a hatchery setting including genetics (Riera-Heredia, Velez, Gutierrez, Navarro, & Capilla, 2019;Sadler, Pankhurst, & King, 2001), the environment (e.g., improper water temperature, toxins, and so on; Koumoundouros et al, 1997;Sfakianakis, Georgakopoulou, Papadakis, Divanach, & Kentouri, and M., Koumoundouros, G., 2006;Cobcroft & Battaglene, 2009), nutrition (e.g., excesses or deficiencies; Takeuchi, 2014;Fjelldal et al, 2016;Shefat, 2018), and husbandry (e.g., density, physical trauma, excessive water currents, and so on; .…”

Section: Noninfectious Disease and Fish Qualitymentioning

confidence: 99%

The status of white seabass, Atractoscion nobilis as a commercially ready species for marine US aquaculture

Drawbridge

Shane

Silbernagel

2021

J World Aquaculture Soc

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The white seabass, Atractoscion nobilis, is a member of the family Sciaenidae, which includes croakers and drums.Atractoscion nobilis have numerous aquaculture characteristics desirable for commercialization, including excellent market appeal. Consumer and market surveys of farmed A. nobilis have consistently yielded reviews of 'good to excellent' when rated for taste, texture, appearance, and freshness. Wild adult A. nobilis adapt readily to captivity, are highly fecund batch spawners (100,000 eggs/kg of female), will spawn out of season, and produce eggs with high viability (median 73%) and with good hatching rates (median 88%). Atractoscion nobilis larvae are first-feed Artemia in clear water and weaning is complete by 30 days post hatch (dph). Survival of A. nobilis from unhatched egg to 50 dph is consistently 20-40%. A market size of 1.0 kg has been achieved in 18 months. Infectious diseases are uncommon and are effectively mitigated using recirculating systems with appropriate biosecurity. Atractoscion nobilis are highly susceptible to gas bubble disease, which is mitigated by vacuum degassing, culture in cooler temperatures, or using deep culture units like net pens. Commercial culture of A. nobilis will benefit from selective breeding programs, custom diet formulations, and more health management tools.

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Gene expression analyses in malformed skeletal structures of gilthead sea bream (Sparus aurata)

Cited by 12 publications

References 62 publications

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Muscle regeneration in gilthead sea bream: Implications of endocrine and local regulatory factors and the crosstalk with bone

The status of white seabass, Atractoscion nobilis as a commercially ready species for marine US aquaculture

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