Gene editing in Brassica napus for basic research and trait development

Gocal, Greg F. W.

doi:10.1007/s11627-021-10212-1

Cited by 10 publications

(8 citation statements)

References 119 publications

(183 reference statements)

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“…In contrast with RNAi-based approaches which are leading to a knock down of expression GE approaches can lead to a complete knock out of the expression of selected genes. These GE technologies might have a promising perspective for application in molecular breeding of oilseed rape (summarised in Chang et al 2021 ; Gocal 2021 ). Many examples for different plant species exist where genome editing was used to induce plant resistance against fungal pathogens, e.g.…”

Section: Breeding Resources and Breeding Strategiesmentioning

confidence: 99%

Perspectives for integrated insect pest protection in oilseed rape breeding

et al. 2022

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In the past, breeding for incorporation of insect pest resistance or tolerance into cultivars for use in integrated pest management schemes in oilseed rape/canola (Brassica napus) production has hardly ever been approached. This has been largely due to the broad availability of insecticides and the complexity of dealing with high-throughput phenotyping of insect performance and plant damage parameters. However, recent changes in the political framework in many countries demand future sustainable crop protection which makes breeding approaches for crop protection as a measure for pest insect control attractive again. At the same time, new camera-based tracking technologies, new knowledge-based genomic technologies and new scientific insights into the ecology of insect–Brassica interactions are becoming available. Here we discuss and prioritise promising breeding strategies and direct and indirect breeding targets, and their time-perspective for future realisation in integrated insect pest protection of oilseed rape. In conclusion, researchers and oilseed rape breeders can nowadays benefit from an array of new technologies which in combination will accelerate the development of improved oilseed rape cultivars with multiple insect pest resistances/tolerances in the near future.

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Section: Breeding Resources and Breeding Strategiesmentioning

confidence: 99%

Perspectives for integrated insect pest protection in oilseed rape breeding

et al. 2022

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“…Conventional plant breeding strategies that have been used in the past and can be used involve evaluating and selecting silique shattering resistant parental plant traits with the objective of combining those traits to increase performance (Gocal, 2021). Many literatures on the successful application of CRISPR/Cas9 technology in rapeseed breeding have been published as of 2022, indicating that this technology's application in rapeseed is becoming more mature, and it has been widely used in the creation of germplasm resources and genetic improvement of rapeseed.…”

Section: Agronomic Managementmentioning

confidence: 99%

“…Many literatures on the successful application of CRISPR/Cas9 technology in rapeseed breeding have been published as of 2022, indicating that this technology's application in rapeseed is becoming more mature, and it has been widely used in the creation of germplasm resources and genetic improvement of rapeseed. In B. napus, CRISPR/Cas9 technology has become a key tool for studying gene function and molecular mechanisms (Ali et al, 2014;Bortesia and Fischer, 2015;Brookes and Barfoot, 2018;Cabral et al, 2018;Bernabé-Orts et al, 2019;Gocal, 2021).…”

Section: Agronomic Managementmentioning

confidence: 99%

Genetic and Physiological Aspects of Silique Shattering in Rapeseed and Mustard

MUSTAFA¹,

MAHMOOD²,

BASHIR³

et al. 2022

SABRAOJBG

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Rapeseed (Brassica napus L.) and mustard (Brassica juncea L.) are two important oilseed crops grown worldwide for edible oil and meal production, as well as, a source of renewable energy. Silique shattering at the maturity stage is the major cause of seed yield reduction in brassica. Losses in seed yield are more in developing countries due to poor management and the non-availability of combine harvesters. Silique shattering resistance is essential for achieving good seed yield especially in Brassica napus. The silique on plants of rapeseed and mustard mature in different phases due to indeterminate growth habit, which is also a reason for shattering losses. Silique shattering is linked with the creation of a dehiscence zone in a brassica pod. When the siliqua wall loses its hydration, along the length of the siliqua, a few cell layers separate the replum from the pericarp tip of the two silique valves. In the dehiscence zone, it involves the collapse of cell walls and cell separation, as well as, the destruction of the middle lamella and enhanced hydrolytic enzyme activity. To avoid seed yield losses, resistance against silique shattering is essential in rapeseed and mustard cultivars. There are multiple QTLs discovered that control variance in silique shattering. Previous studies validated the shattering process in the model plant Arabidopsis thaliana was controlled by eight different genes. However, their role in controlling silique shattering in rapeseed and mustard is unknown. Modern tools of mutation breeding and genetic engineering, especially CRISPR/Cas9 technology, can be utilized to identify the genetic source for shattering resistance in rapeseed and mustard, which will be helpful for the development of silique-shattering

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“…Afterwards, other effective protocols have been developed, also based on the use of cotyledons [ 21 ] or other explants such as stem portions [ 22 ], protoplasts [ 23 ] or hypocotyl sections [ 24 ]. Among the different selection markers available, kanamycin has been commonly used as an effective selection agent in B. napus transformation, whereas the use of BASTA has been less frequent [ 25 ]. The third major factor is the genotype.…”

Section: Introductionmentioning

confidence: 99%

The Highly Embryogenic Brassica napus DH4079 Line Is Recalcitrant to Agrobacterium-Mediated Genetic Transformation

Calabuig-Serna

Mir

Porcel

et al. 2023

Plants

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Brassica napus is a species of high agronomic interest, used as a model to study different processes, including microspore embryogenesis. The DH4079 and DH12075 lines show high and low embryogenic response, respectively, which makes them ideal to study the basic mechanisms controlling embryogenesis induction. Therefore, the availability of protocols for genetic transformation of these two backgrounds would help to generate tools to better understand this process. There are some reports in the literature showing the stable transformation of DH12075. However, no equivalent studies in DH4079 have been reported to date. We explored the ability of DH4079 plants to be genetically transformed. As a reference to compare with, we used the same protocols to transform DH12075. We used three different protocols previously reported as successful for B. napus stable transformation with Agrobacterium tumefaciens and analyzed the response of plants. Whereas DH12075 plants responded to genetic transformation, DH4079 plants were completely recalcitrant, not producing any single regenerant out of the 1784 explants transformed and cultured. Additionally, an Agrobacterium rhizogenes transient transformation assay was performed on both lines, and only DH12075, but no DH4079 seedlings, responded to A. rhizogenes infection. Therefore, we propose that the DH4079 line is recalcitrant to Agrobacterium-mediated transformation.

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Gene editing in Brassica napus for basic research and trait development

Cited by 10 publications

References 119 publications

Perspectives for integrated insect pest protection in oilseed rape breeding

Perspectives for integrated insect pest protection in oilseed rape breeding

Genetic and Physiological Aspects of Silique Shattering in Rapeseed and Mustard

The Highly Embryogenic Brassica napus DH4079 Line Is Recalcitrant to Agrobacterium-Mediated Genetic Transformation

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