Gene editing applications to modulate crop flowering time and seed dormancy

Kishchenko, Olena; Zhou, Yuzhen; Jatayev, Satyvaldy; Shavrukov, Yuri; Borisjuk, Nikolai

doi:10.1007/s42994-020-00032-z

Cited by 12 publications

(12 citation statements)

References 103 publications

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“…In fact, genetic manipulation of key flowering time genes has increased yield production and nutritional quality in several food and forage crop species (Aung et al, 2015 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Dash and Rai, 2022;Kishchenko et al, 2020;Liu et al, 2021;Lorenzo et al, 2020;Tadege et al, 2015;Weller and Ortega, 2015;Wu et al, 2020;Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Multiplex CRISPR/Cas9‐mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality

Wolabu

Mahmood

Jerez

et al. 2023

Plant Biotechnology Journal

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Summary Alfalfa (Medicago sativa L.) is a perennial flowering plant in the legume family that is widely cultivated as a forage crop for its high yield, forage quality and related agricultural and economic benefits. Alfalfa is a photoperiod sensitive long‐day (LD) plant that can accomplish its vegetative and reproductive phases in a short period of time. However, rapid flowering can compromise forage biomass yield and quality. Here, we attempted to delay flowering in alfalfa using multiplex CRISPR/Cas9‐mediated mutagenesis of FLOWERING LOCUS Ta1 (MsFTa1), a key floral integrator and activator gene. Four guide RNAs (gRNAs) were designed and clustered in a polycistronic tRNA–gRNA system and introduced into alfalfa by Agrobacterium‐mediated transformation. Ninety‐six putative mutant lines were identified by gene sequencing and characterized for delayed flowering time and related desirable agronomic traits. Phenotype assessment of flowering time under LD conditions identified 22 independent mutant lines with delayed flowering compared to the control. Six independent Msfta1 lines containing mutations in all four copies of MsFTa1 accumulated significantly higher forage biomass yield, with increases of up to 78% in fresh weight and 76% in dry weight compared to controls. Depending on the harvesting schemes, many of these lines also had reduced lignin, acid detergent fibre (ADF) and neutral detergent fibre (NDF) content and significantly higher crude protein (CP) and mineral contents compared to control plants, especially in the stems. These CRISPR/Cas9‐edited Msfta1 mutants could be introduced in alfalfa breeding programmes to generate elite transgene‐free alfalfa cultivars with improved forage biomass yield and quality.

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Section: Introductionmentioning

confidence: 99%

Multiplex CRISPR/Cas9‐mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality

Wolabu

Mahmood

Jerez

et al. 2023

Plant Biotechnology Journal

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“…Breeders widely use genomic modeling and editing technologies to address plant breeding problems (Salina, 2016). Modern systems demonstrate the possibility of obtaining nontransgenic plants with specified mutations (Borisuyk et al, 2019), for example editing the genes that control the optimal flowering time of the most important crops (Kishchenko et al, 2020).…”

Section: Molecular Biological Methods In Plant Breedingmentioning

confidence: 99%

Scientific support to plant breeding and seed production in Siberia in the XXI century

Гончаров

2021

Vestn. VOGiS

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Agriculture in the Russian Federation is fundamental to the country’s economic performance, living standards, the wellbeing of people and state safety. Considerations relating to food security, prospects of and challenges before plant breeding in the Siberian Federal District (SFD), the largest agricultural area of the Russian Federation, are provided in the article. The agricultural area used in the SFD is about 50 million hectares and accounts for 13 % of the country’s gross grain production. The need for the introduction of modern molecular biological methods, bioengineering and IT technology is demonstrated and discussed. As Russia as a whole, Siberia is largely engaged in unpromising extensive farming practices, which rely on natural soil fertility, and this factor should be taken into account. Another issue is noncompliance with intensive farming technologies used for cultivating new-generation commercial cultivars. Although capital investments in plant breeding are the most cost effective investments in crop production, breeders’ efforts remain underfunded. The article explains the need for fundamental reform in this economic sector: the recognition of plant breeding as being a fundamental science; a fair increase in its funding; the development of a breeding strategy, nationally and regionally; the further expansion of the network of the Breeding Centers; the re-establishment and improvement of the universities’ departments specialized in plant breeding and seed production; having more state-funded places in the universities for training plant breeders to be able to maintain and cement the country’s advanced position in plant breeding and to develop new globally competitive next-generation cultivars of main crops. Should these issues be ignored, all the problems that have accumulated to date will lead to risks of long-term instability in this economic sector. The need for the careful preservation of continuity in plant breeders and plants being bred is stated. The regulatory functions of the state and agricultural science in plant breeding, plant industry and seed production are considered.

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“…In the absence of delayed germination mechanisms, pre-harvest sprouting may occur among grains still on the mother plant, usually in response to rain and causing substantial economic loss [35]. Therefore, modern wheat and barley crop improvement is seeking to selectively re-introduce seed dormancy [36]. Thus, human activities affecting the timing of germination and maturation continue to be a part of the long-term domestication history of cereals.…”

Section: Germinationmentioning

confidence: 99%

A Time to Sow, a Time to Reap: Modifications to Biological and Economic Rhythms in Southwest Asian Plant and Animal Domestication

et al. 2022

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This paper reviews changes to lifecycle temporality in Southwest Asian plant and animal domestication, exploring their relationship to long-term processes associated with ancient and contemporary globalization. We survey changes under domestication to the timing of seed dispersal, germination, vegetative growth, flowering and maturation in wheat and barley and to birth, reproduction, lactation, wool production and death in sheep, goats and cattle. Changes in biological temporality among domesticates are ultimately related to globally increasing production intensity, geographic diffusion, and agricultural diversity associated with cultivar/breed evolution. Recently, however, increasing crop production intensity and geographic diffusion are accompanied by declining agricultural diversity worldwide. Similar processes are apparent in contemporary economic and cultural globalization, suggesting that long-term agricultural developments might be viewed as a subset of globalization. Moreover, the origin of certain features of contemporary globalization may be traced back to the beginnings of plant and animal domestication. Thus, while biologists since Darwin considered domestication as a model for the study of evolution, we suggest that domestication may also offer a model for the study of globalization.

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Gene editing applications to modulate crop flowering time and seed dormancy

Cited by 12 publications

References 103 publications

Multiplex CRISPR/Cas9‐mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality

Multiplex CRISPR/Cas9‐mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality

Scientific support to plant breeding and seed production in Siberia in the XXI century

A Time to Sow, a Time to Reap: Modifications to Biological and Economic Rhythms in Southwest Asian Plant and Animal Domestication

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