Gene Editing and Systems Biology Tools for Pesticide Bioremediation: A Review

Abstract

Bioremediation is the degradation potential of microorganisms to dissimilate the complex chemical compounds from the surrounding environment. The genetics and biochemistry of biodegradation processes in datasets opened the way of systems biology. Systemic biology aid the study of interacting parts involved in the system. The significant keys of system biology are biodegradation network, computational biology, and omics approaches. Biodegradation network consists of all the databases and datasets which aid in assisting the degradation and deterioration potential of microorganisms for bioremediation processes. This review deciphers the bio-degradation network, i.e., the databases and datasets (UM-BBD, PAN, PTID, etc.) aiding in assisting the degradation and deterioration potential of microorganisms for bioremediation processes, computational biology and multi omics approaches like metagenomics, genomics, transcriptomics, proteomics, and metabolomics for the efficient functional gene mining and their validation for bioremediation experiments. Besides, the present review also describes the gene editing tools like CRISPR Cas, TALEN, and ZFNs which can possibly make design microbe with functional gene of interest for degradation of particular recalcitrant for improved bioremediation.

Design, Synthesis, and Antimicrobial Evaluation of New Annelated Pyrimido[2,1-c][1,2,4]triazolo[3,4-f][1,2,4]triazines

“…Molecules 2020, 25, x FOR PEER REVIEW 2 of 17 phenyl-6-(thiophen-2-yl)-6,7-dihydro-2H-pyrimido[2,1-c] [1,2,4]triazine-3,4-dione tripod-based congeners.…”

“…Genetic mutations are major contributors to the prognosis of drug-resistant microbial strains [1]. These strains are, in most cases, able to detoxify drugs using mutant digestive enzymes like β-lactamases [2].…”

“…Due to our interest in these azines, their ligation, and modification, we revisited and continued upon our previous research projects [24][25][26][27][28][29][30] with this study. We describe the annulation and in vitro preliminary antimicrobial impact of new 8-phenyl-6-(thiophen-2-yl)-6,7-dihydro-2H-pyrimido[2,1-c] [1,2,4]triazine-3,4-dione tripod-based congeners.…”

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A series of 34 new pyrimido[2,1-c] [1,2,4]triazine-3,4-diones were synthesized and fully characterized using IR, NMR, MS, and microanalytical analysis. In vitro investigation of 12 compounds of this series revealed promising antimicrobial activity of the conjugates 15a and 15f-j that were tagged with electron-withdrawing groups, with sensitivities ranging from 77% to as high as 100% of the positive control.

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CONTROLE DE Sclerotinia sclerotiorum E Fusarium sp. COM ÓLEO ESSENCIAL DE MELALEUCA

“…O surgimento de pragas é um reflexo direto de desequilíbrios provocados pelos monocultivos, especialmente naqueles que não utilizam sistemas de rotação de culturas. O uso contínuo e intensivo de pesticidas na agricultura é mais um fator que interfere negativamente sobre o ambiente, pois embora apresente efeito imediato sobre a redução da população de pragas nos cultivos, possui efeitos negativos a médio e longo prazo (MOORMAN, 2018;BOUDH ;JAISWAL et al, 2019). Isto porque a maioria dos agrotóxicos atua não somente nos organismos alvo, mas também sobre organismos benéficos que atuam naturalmente no controle biológico de pragas.…”