Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana

Gasmi, Laila; Baek, Sehyeon; Kim, Jong Cheol; Kim, Sihyeon; Lee, Mi Rong; Park, So Eun; Shin, Tae Young; Lee, Se Jin; Parker, Bruce L.

doi:10.1038/s41598-020-78910-1

Cited by 19 publications

(11 citation statements)

References 61 publications

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“…reported that B. bassiana ERL836 highly activated two metabolic pathways: plasma membrane‐mediated transportation and fatty acid degradation, by catalysis of cytochrome P450. Recent studies of genetic diversity have indicated that lectin‐like, mucin‐induced signaling‐associated, biotrophy‐associated, and chitinase genes were required for the EPFs' virulence 43 . It should be determined whether or not the genes described above contributed to the rapid Bb ‐eGFP conidial adhesion, germination, germ tuber formation, production of penetration peg (a kind of hypha), and early penetration of the T. castaneum larval body cuticles.…”

Section: Resultsmentioning

confidence: 99%

Ultrastructural analysis of beetle larva cuticles during infection with the entomopathogenic fungus, Beauveria bassiana

Baek

Noh

Mun

et al. 2022

Pest Management Science

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BACKGROUND: Beauveria bassiana is one of the commercially available entomopathogenic fungi (EPF), and a number of isolates with high virulence and broad host spectrum have been used to control agricultural and forest pests. Although the functional importance of genes in EPFs' pathogenesis have been extensively studied, the precise ultrastructural mechanism of the fungal infection, particularly penetration of the host insect cuticles, is not well understood.RESULTS: In this study, we investigated the morphology and ultrastructure of the larval cuticle of the red flour beetle, Tribolium castaneum, after treatment with B. bassiana ERL1170 expressing an enhanced green fluorescent protein (Bb-eGFP). The Bb-eGFP showed high virulence against the larvae, with approximately 90% mortality at 48 h after treatment (HAT) and 100% at 72 HAT under our infection conditions. In these larvae, the regions of the body wall with flexible cuticles, such as the ventral and ventrolateral thorax and abdomen, became darkly melanized, but there was little to no melanization in the rigid dorsal cuticular structures. Confocal microscopy and transmission electron microscopy (TEM) indicated that germinated conidia on the surface of the larval cuticle were evident at 6 HAT, which formed penetration pegs and began to penetrate the several cuticle layers/laminae by 12 HAT. The penetration pegs then developed invading hyphae, some of which passed through the cuticle and reached the epidermal cells by 24 HAT. The larval cuticle was aggressively and extensively disrupted by 48 HAT, and a number of outgrowing hyphae were observed at 72 HAT. CONCLUSIONS: Our results indicate that Bb-eGFP is capable of infection and penetrating T. castaneum larvae shortly after inoculation (∼24 HAT) at the body regions with apparently flexible and membranous cuticles, such as the ventral intersegmental regions and the ventrolateral pleura. This study provides details on the histopathogenesis of the host cuticle by infection and penetration of EPFs, which can facilitate the management of insect pests.

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Section: Resultsmentioning

confidence: 99%

Ultrastructural analysis of beetle larva cuticles during infection with the entomopathogenic fungus, Beauveria bassiana

Baek

Noh

Mun

et al. 2022

Pest Management Science

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“…Zhang et al (2020) examined the genetic difference among B. bassiana isolates from different hosts by phylogenetic analysis and determined genetic differences between the virulent isolates. In a more recent study, Gasmi et al (2021) characterized the variety of genes involved in diverse mechanisms of the infective cycle of 42 isolates that have different thermo-tolerance, growth rates, and virulence. The analyzed genes showed general genetic diversity measured as and copy number variation (CNV) and non-synonymous changes (NSC) with most of them being subjected to positive episodic diversifying selection.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of indigenous isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) against the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Baki¹,

Tosun

Erler

2021

Egypt J Biol Pest Control

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Background The Colorado potato beetle (CPB) Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is one of the most damaging pests of potatoes all over the world as well as in Turkey. Both adults and larvae of this pest feed on the foliage of potatoes, and often cause complete defoliation of potato plants, with considerable yield losses of up to 60%. Chemical control of this pest has induced the selection of resistant CPB populations and negative environmental impact. The aim of the present study was to evaluate the pathogenicity of 14 Turkish isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) against different stages of CPB under laboratory conditions. Results All isolates were tested as conidial suspensions of (1 × 107 conidia/ml), using spray method. The results from pathogenicity assays showed that virulence of tested isolates was variable, and mortality increased with the time of exposure. The highest mortality was seen in larvae of CPB; however, young larvae (1st and 2nd instars) were more susceptible to fungal isolates than older ones (3rd and 4th instars). Four isolates (BbDm-1, BbDs-2, BbMg-2, and BbMp-1) were more pathogenic than others against L. decemlineata, causing mortalities between 96.7 and 100% in the 2 youngest larval instars, respectively, between 91.7 and 96.7% in the 2 oldest larval instars, respectively, and between 93.3 and 96.7% in 0–48-h-old adults, respectively, 9 days post treatment. Furthermore, these 4 isolates had the most potent egg-hatching inhibitory effects (66.7–90%). The results of molecular phylogenetic analyses based on the ITS region sequence indicated that all tested B. bassiana isolates showed a high similarity (99%) with other B. bassiana isolates in GenBank. Conclusions Results suggested that the 4 B. bassiana isolates were highly virulence and had the potential for biological control of CPB. Further tests under field conditions are recommended to validate the laboratory results.

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“…Be. bassiana sensu stricto is generally recognised as a globally distributed, genetically diverse species or species complex [69,70].…”

Section: Discussionmentioning

confidence: 99%

Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in Brassica

et al. 2021

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Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of Brassica, identified as Beauveria bassiana and Pseudogymnoascus pannorum, were inoculated into cultivars of three Brassica species—Brassica napus, Br. rapa and Br. oleracea. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant–endophyte associations formed between Brassica plants and Be. bassiana significantly inhibited phoma stem canker, a devastating disease of Brassica crops worldwide, caused by the fungal pathogen Leptosphaeria maculans. The novel association formed with P. pannorum significantly suppressed the amount of disease caused by L. maculans in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops.

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Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana

Cited by 19 publications

References 61 publications

Ultrastructural analysis of beetle larva cuticles during infection with the entomopathogenic fungus, Beauveria bassiana

Ultrastructural analysis of beetle larva cuticles during infection with the entomopathogenic fungus, Beauveria bassiana

Efficacy of indigenous isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) against the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in Brassica

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