Gene disruption in Lactobacillus plantarum strain 80 by site-specific recombination: isolation of a mutant strain deficient in conjugated bile salt hydrolase activity

Leer, R.J.; Christiaens, H.; Verstraete, Willy; Peters, L K; Posno, M.; Pouwels, P.H.

doi:10.1007/bf00281627

Cited by 47 publications

(18 citation statements)

References 19 publications

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“…DNA cloning and sequencing. Total DNA from L. plantarum B21 was prepared as described previously (22), partially digested with Sau3A1, and size fractionated through a sucrose gradient as described previously (32). DNA fragments of 5 kb (average size) were ligated into the BamHI site of plasmid pKK232 (4), and the ligation mixture was used to transform E. coli FA31 competent cells.…”

Section: Methodsmentioning

confidence: 99%

Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression

Marasco

Muscariello

Varcamonti³

et al. 1998

J Bacteriol

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A newly identified bglH gene coding for a phospho-β-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. The bglH gene was shown to be expressed on a monocistronic transcriptional unit. Its transcription was repressed 10-fold in L. plantarum cells grown on glucose compared to the β-glucoside salicin as a sole carbon source. A catabolite-responsive element (CRE) spanning from −3 to +11 with respect to the transcriptional start point was found, and its functionality was assessed by mutational analysis. In vitro and in vivo DNA binding experiments suggested the occurrence of a DNA-protein complex at the CRE site, which would mediate glucose repression ofbglH expression.

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Section: Methodsmentioning

confidence: 99%

Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression

Marasco

Muscariello

Varcamonti³

et al. 1998

J Bacteriol

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“…Resistance to bile is another trait observed in gastrointestinal-related LAB. Bile-specific hydrolases and transporters have been found in many probiotic species such as L. johnsonii, L. acidophilus, Lactobacillus reuteri, and L. plantarum (Leer et al, 1993;Pridmore et al, 2004;McAuliffe et al, 2005;Martoni et al, 2008). Microarray expression analysis of L. acidophilus NCFM found 289 genes that were differentially expressed in the presence of bile.…”

Section: Probiotic and Gastrointestinal-related Genesmentioning

confidence: 99%

Genomics of lactic acid bacteria

Schroeter

Klaenhammer

2009

FEMS Microbiology Letters

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Lactic acid bacteria (LAB) are found to occupy a variety of ecological niches including fermented foods as well as mucosal surfaces of humans and other vertebrates. This review is based on the genomic content of LAB that is responsible for the functional and ecological diversity of these bacteria. These genomes reveal an ongoing process of reductive evolution as the LAB have specialized to different nutritionally rich environments. Species-to-species variation in the number of pseudogenes as well as genes directing nutrient uptake and metabolism reflects the adaptation of LAB to food matrices and the gastrointestinal tract. Although a general trend of genome reduction was observed, certain niche-specific genes appear to be recently acquired and appear on plasmids or adjacent to prophages. Recent work has improved our understanding of the genomic content responsible for various phenotypes that continue to be discovered, as well as those that have been exploited by man for thousands of years.

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“…2). Electrotransformation of L. plantarum LM3 cells with the plasmids was performed as described previously [23]. The L. plantarum DNA inserts of the four plasmids were sequenced to verify that no other mutation besides the ones described above had been selected.…”

Section: Plasmid Constructionmentioning

confidence: 99%

Mutational analysis of the bglH catabolite-responsive element (cre) in Lactobacillus plantarum

Marasco

2002

FEMS Microbiology Letters

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Catabolite repression of the Lactobacillus plantarum bglH gene is mediated by the catabolite control protein A (CcpA). Here we show that the binding site for the protein is a catabolite-responsive element (cre) sequence overlapping the start site of transcription. Two single and one double base substitutions in the cre sequence caused derepression of a plasmid-borne bglH^cat transcriptional fusion in L. plantarum cells grown on glucose. Analysis of the single mutations indicates that the G and C nucleotide residues in positions 2 and 13, respectively, of the 14-bp cre sequence are required for catabolite repression. ß

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Gene disruption in Lactobacillus plantarum strain 80 by site-specific recombination: isolation of a mutant strain deficient in conjugated bile salt hydrolase activity

Cited by 47 publications

References 19 publications

Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression

Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression

Genomics of lactic acid bacteria

Mutational analysis of the bglH catabolite-responsive element (cre) in Lactobacillus plantarum

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