Gene delivery to the heart by magnetic nanobeads

Li, Wenzhong; Nesselmann, Catharina; Zhou, Zhao‐Hui; Ong, Lawrence; Öri, Ferenc; Tang, Guping; Kaminski, Alexander; Lützow, Karola; Lendlein, Andreas; Liebold, Andreas; Stamm, Christof; Wang, John; Steinhoff, Gustav; Ma, Nan

doi:10.1016/j.jmmm.2006.11.201

Cited by 23 publications

(14 citation statements)

References 9 publications

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“…Moieties that are responsive to different incentives must be combined to result in an appropriate set of diverse trigger mechanisms at different time points. [193] Such moieties might be cleaved in acidic environments, show good buffering capacity, be redox active or enzymatically cleavable, or be stimulated by some extragenous stimuli such as heat, [194] magnetic fields, [195] or irradiation. [196] A promising example of a triggerable system was created by the synthesis of a liposomal carrier that was comprised of a membrane-permeable ligand and a reductively detachable PEG coating.…”

Section: Gene-delivery Systems With Multiple Triggerable Functionalitiesmentioning

confidence: 99%

Delivery of Nucleic Acids via Disulfide‐Based Carrier Systems

et al. 2009

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Nucleic acids are not only expected to assume a pivotal position as "drugs" in the treatment of genetic and acquired diseases, but could also act as molecular cues to control the microenvironment during tissue regeneration. Despite this promise, the efficient delivery of nucleic acids to their side of action is still the major hurdle. One among many prerequisites for a successful carrier system for nucleic acids is high stability in the extracellular environment, accompanied by an efficient release of the cargo in the intracellular compartment. A promising strategy to create such an interactive delivery system is to exploit the redox gradient between the extra- and intracellular compartments. In this review, emphasis is placed on the biological rationale for the synthesis of redox sensitive, disulfide-based carrier systems, as well as the extra- and intracellular processing of macromolecules containing disulfide bonds. Moreover, the basic synthetic approaches for introducing disulfide bonds into carrier molecules, together with examples that demonstrate the benefit of disulfides at the individual stages of nucleic acid delivery, will be presented.

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Section: Gene-delivery Systems With Multiple Triggerable Functionalitiesmentioning

confidence: 99%

Delivery of Nucleic Acids via Disulfide‐Based Carrier Systems

et al. 2009

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“…Compared to organic nanoparticles, inorganic nanoparticles hold some advantages including easiness for preparation, low cytotoxicity and high stability. Several types of inorganic nanoparticles have been employed as gene transfer carriers, including calcium phosphate [49], carbon nanotubes [53], magnetic nanobeads [99,100], silica [11], gold [56] and quantum dots [156]. Hydroxyapatite nanoparticles mediated gene delivery into rat MSCs resulted in a significantly decreased cytotoxicity compared to cationic lipid, although the transfection efficiency was relatively lower [30].…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Genetic engineering of mesenchymal stem cells by non-viral gene delivery

Wang

et al. 2014

Clinical Hemorheology and Microcirculation

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Mesenchymal stem cells (MSCs) are an ideal cell source for tissue engineering and regenerative medicine as they possess self-renewal properties and multilineage differentiation potential. They can be isolated from various tissues and expanded easily through normal cell culture techniques. Genetic modifications of MSCs to further improve their therapeutic efficacy have been widely studied and extensively researched. Compared to viral gene delivery methods, non-viral methods generate less toxicity and immunogenicity and thus represent a promising and effective tool for the genetic engineering of MSCs. In the last decades, various non-viral gene delivery strategies have been developed and some of them have been applied for MSC transfection. This paper gives an overview of the techniques, influencing factors and potential applications of non-viral methods used for the genetic engineering of MSCs.

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“…Containing multiple primary, secondary, and tertiary amines, PEI has strong affinity toward the IONP surface and can easily form conjugates with IONPs. The resulting adducts are highly positively charged in aqueous solution and have been exploited as cell labeling agents [74] and as transfection agents [46,75]. Compared with PEI alone, these PEI-IONP conjugates may have enhanced cellular permeability, if used in conjunction with a vertically oriented magnetic field [76].…”

Section: Polymer and Protein Adsorptionmentioning

confidence: 99%

Nanoparticle Surface Modification and Bioconjugation

Xie

Gao

Michalski

et al. 2011

Nanoplatform‐Based Molecular Imaging

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The last decade has witnessed fast progress in nanotechnology. Especially, the marriage of nanotechnology and conventional imaging facilities has brought forth a new research realm that holds great potential in bringing revolutionary changes to the current diagnostic technologies. The interface that connects nanomaterials and functional biomolecules plays a critical role in determining the performance of nanoconjugates in living subjects, and a lot of effort has been directed to this area. This chapter attempts to review the current status of biomolecule-nanoparticle conjugate preparation and use, with an emphasis on introducing the methodologies that have been developed to achieve a physiologically stable and functioning extendable nanoparticle coating. INTRODUCTIONNanoparticle-based contrast agents, with their unique size (several to hundreds of nanometers in diameter) and physical properties (magnetic, optical, acoustic, etc.), hold great promise for revolutionizing current radiologic methodologies by offering more accurate, specific, and detailed imaging at both the cellular and subcellular levels. Recent Nanoplatform-Based Molecular Imaging Edited by Xiaoyuan Chen

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Gene delivery to the heart by magnetic nanobeads

Cited by 23 publications

References 9 publications

Delivery of Nucleic Acids via Disulfide‐Based Carrier Systems

Delivery of Nucleic Acids via Disulfide‐Based Carrier Systems

Genetic engineering of mesenchymal stem cells by non-viral gene delivery

Nanoparticle Surface Modification and Bioconjugation

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