Gene delivery and immunomodulatory effects of plasmid DNA associated with Branched Amphiphilic Peptide Capsules

Avila, L. Adriana; Aps, Luana R.M.M.; Nicoleta, Ploscariu,; Sukthankar, Pinakin; Guo, Rui; Wilkinson, K.E.; Games, Patrícia Dias; Szoszkiewicz, Robert; Alves, Rúbens Prince dos Santos; Diniz, Mariana O.; Fang, Yuanyuan; Ferreira, Luís Carlos de Souza; Tomich, John M.

doi:10.1016/j.jconrel.2016.08.042

Cited by 37 publications

(56 citation statements)

References 44 publications

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“…The capsules are water soluble, stable in blood and made entirely of natural amino acids (Sukthankar et al ., 2014). The size of the capsules range from 10–50 nm (depending on annealing) and are resistant to detergents and proteases (Sukthankar et al ., 2013; Avila et al ., 2016). Additionally, the capsules interact with DNA and RNA acting as cationic nucleation centres with the negatively charged DNA binding to the outer surface.…”

Section: Introductionmentioning

confidence: 99%

Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR‐Cas9 ribonucleoprotein

Chaverra-Rodríguez

Benetta

Heu

et al. 2020

Insect Molecular Biology

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CRISPR/Cas9 gene editing is a powerful technology to study the genetics of rising model organisms, such as the jewel wasp Nasonia vitripennis. However, current methods involving embryonic microinjection of CRISPR reagents are challenging. Delivery of Cas9 ribonucleoprotein into female ovaries is an alternative that has only been explored in a small handful of insects, such as mosquitoes, whiteflies and beetles. Here, we developed a simple protocol for germline gene editing by injecting Cas9 ribonucleoprotein in adult N. vitripennis females using either ReMOT control (Receptor-Mediated Ovary Transduction of Cargo) or BAPC (Branched Amphiphilic Peptide Capsules) as ovary delivery methods. For ReMOT Control we used the Drosophila melanogasterderived peptide 'P2C' fused to EGFP to visualize the ovary delivery, and fused to Cas9 protein for gene editing of the cinnabar gene using saponin as an endosomal escape reagent. For BAPC we optimized the concentrations of protein, sgRNA and the transfection reagent. We demonstrate delivery of protein cargo such as EGFP and Cas9 into developing oocytes via P2C peptide and BAPC. Additionally, somatic and germline gene editing were demonstrated. This approach will greatly facilitate CRISPR-applied genetic manipulation in this and other rising model organisms.

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Section: Introductionmentioning

confidence: 99%

Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR‐Cas9 ribonucleoprotein

Chaverra-Rodríguez

Benetta

Heu

et al. 2020

Insect Molecular Biology

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“…DNA or mRNA encoding antigens are commonly used when combined with appropriate carriers to avoid degradation by nucleases . Avila et al . synthesized branched amphiphilic peptides capsules (BAPCs) to delivery DNA vaccine against type 16 human papilloma virus (HPV‐16).…”

Section: Therapeutic Genes Targeting the Tumor Microenvironmentmentioning

confidence: 99%

“…DNA or mRNA encoding antigens are commonly used when combined with appropriate carriers to avoid degradation by nucleases. 104 Avila et al 105 They exist in the form of monomer or multimers and play a biological role as immunomodulating agents. 111,112 Cytokines can be produced not only by lymphocytes, but also by monocytes, as well as fibroblasts.…”

Section: Targeting Tumor-associated Antigensmentioning

confidence: 99%

Tumor microenvironment as the “regulator” and “target” for gene therapy

Chen

Feng

et al. 2019

The Journal of Gene Medicine

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In this review, we focus on strategies for designing functional nano gene carriers, as well as choosing therapeutic genes targeting the tumor microenvironment. Gene mutations have a great impact on the occurrence of cancer. Thus, gene therapy plays a major role in cancer therapy and has the potential to cure cancer. Well‐designed gene therapy largely relies on effective gene carriers, which can be divided into viral carriers and non‐viral carriers. A gene carrier delivers functional genes to their intracellular target and avoids nucleic acids being degraded by nucleases in the serum. Most conventional cancer gene therapies only target cancer cells and do not appear to be sufficintly efficient to pass clinical trials. Accumulating evidence has shown that extending the therapeutic strategies to the tumor microenvironment, rather than the tumor cell itself, can allow more options for achieving robust anti‐cancer efficiency. In addition, unusual features between tumor microenvironment and normal tissues, such as a lower pH, higher glutathione and reactive oxygen species concentrations, and overexpression of some enzymes, facilitate the design of smart stimuli‐responsive gene carriers regulated by the tumor microenvironment. These carriers interact with nucleic acids and then form stable nanoparticles under physiological conditions. By regulation of the tumor microenvironment, stimuli‐responsive gene carriers are able to change their properties and achieve high gene delivery efficiency. Considering the tumor microenvironment as the “regulator” and “target” when designing gene carriers and choosing therapeutic genes shows significant benefit with respect to improving the accuracy and efficiency of cancer gene therapy.

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“…The cell fixation alters the transduction of cationic peptides and internalization is an energy-dependent process [30,31]. The use of these peptides has been attempted for the delivery of drugs, genes, peptides and proteins in targeted sites [32,33,34,35].…”

Section: Types Of Ph-responsive Polypeptidesmentioning

confidence: 99%

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

et al. 2019

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Smart nano-carriers have attained great significance in the biomedical field due to their versatile and interesting designs with different functionalities. The initial stages of the development of nanocarriers mainly focused on the guest loading efficiency, biocompatibility of the host and the circulation time. Later the requirements of less side effects with more efficacy arose by attributing targetability and stimuli-responsive characteristics to nano-carriers along with their bio- compatibility. Researchers are utilizing many stimuli-responsive polymers for the better release of the guest molecules at the targeted sites. Among these, pH-triggered release achieves increasing importance because of the pH variation in different organ and cancer cells of acidic pH. This specific feature is utilized to release the guest molecules more precisely in the targeted site by designing polymers having specific functionality with the pH dependent morphology change characteristics. In this review, we mainly concert on the pH-responsive polypeptides and some interesting nano-carrier designs for the effective theranostic applications. Also, emphasis is made on pharmaceutical application of the different nano-carriers with respect to the organ, tissue and cellular level pH environment.

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Gene delivery and immunomodulatory effects of plasmid DNA associated with Branched Amphiphilic Peptide Capsules

Cited by 37 publications

References 44 publications

Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR‐Cas9 ribonucleoprotein

Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR‐Cas9 ribonucleoprotein

Tumor microenvironment as the “regulator” and “target” for gene therapy

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

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