Gene conversion disparity in yeast:its extent, multiple origins, and effects onallele frequencies

Lamb, Bernard Charles

doi:10.1038/sj.hdy.6883310

Cited by 2 publications

(2 citation statements)

References 18 publications

(48 reference statements)

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“…Allelic gene conversion is a molecular mechanism associated with recombination in which a genomic fragment is ‘copied/pasted’ onto another homologous fragment. Little is known about gene conversion in fungi, although it is associated with non-Mendelian segregation ratios in yeast and mammals 43 , 44 . In fungi, previous studies have shown that gene conversion leads to 3:1 ratios in heteroallelic crosses 45 .…”

Section: Resultsmentioning

confidence: 99%

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Borgognone¹,

Castanera²,

Muguerza³

et al. 2017

DNA Res

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Helitrons constitute a superfamily of DNA transposons that were discovered in silico and are widespread in most eukaryotic genomes. They are postulated to mobilize through a “rolling-circle” mechanism, but the experimental evidence of their transposition has been described only recently. Here, we present the inheritance patterns of HELPO1 and HELPO2 helitron families in meiotically derived progeny of the basidiomycete Pleurotus ostreatus. We found distorted segregation patterns of HELPO2 helitrons that led to a strong under-representation of these elements in the progeny. Further investigation of HELPO2 flanking sites showed that gene conversion may contribute to the elimination of such repetitive elements in meiosis, favouring the presence of HELPO2 vacant loci. In addition, the analysis of HELPO2 content in a reconstructed pedigree of subclones maintained under different culture conditions revealed an event of helitron somatic transposition. Additional analyses of genome and transcriptome data indicated that P. ostreatus carries active RNAi machinery that could be involved in the control of transposable element proliferation. Our results provide the first evidence of helitron mobilization in the fungal kingdom and highlight the interaction between genome defence mechanisms and invasive DNA.

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Section: Resultsmentioning

confidence: 99%

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Borgognone¹,

Castanera²,

Muguerza³

et al. 2017

DNA Res

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“…Such studies are needed to clear up the published discrepancies exposed here as well as to prevent the occurrence of further confusions in the yeast meiosis literature. It might also stimulate analyses of the possible importance of epigenetic DSB disparity in genomic studies such as those of allele frequencies in populations (Lamb 1998) or of the fate of newly introduced alleles in finite populations (Nagylaki 1983). …”

Section: Discussionmentioning

confidence: 99%

Apparent Epigenetic Meiotic Double-Strand-Break Disparity in Saccharomyces cerevisiae: A Meta-Analysis

et al. 2016

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Previously published, and some unpublished, tetrad data from budding yeast (Saccharomyces cerevisiae) are analyzed for disparity in gene conversion, in which one allele is more often favored than the other (conversion disparity). One such disparity, characteristic of a bias in the frequencies of meiotic double-strand DNA breaks at the hotspot near the His4 locus, is found in diploids that undergo meiosis soon after their formation, but not in diploids that have been cloned and frozen. Altered meiotic DNA breakability associated with altered metabolism-related chromatin states has been previously reported. However, the above observations imply that such differing parental chromatin states can persist through at least one chromosome replication, and probably more, in a common environment. This conclusion may have implications for interpreting changes in allele frequencies in populations.

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Gene conversion disparity in yeast:its extent, multiple origins, and effects onallele frequencies

Cited by 2 publications

References 18 publications

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Apparent Epigenetic Meiotic Double-Strand-Break Disparity in Saccharomyces cerevisiae: A Meta-Analysis

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