Gene conversion at the var1 locus on yeast mitochondrial DNA.

Strausberg, Robert L.; Butow, Ronald A.

doi:10.1073/pnas.78.1.494

Cited by 25 publications

(5 citation statements)

References 24 publications

(17 reference statements)

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“…We noted that the VAR1 hotspot is an exception to the observed protein-coding conservation, in agreement with previous reports of gene conversion at this locus ( Strausberg et al 1978 ; Strausberg and Butow 1981 ; Zinn et al 1987 ). This gene encodes a mitochondrial protein of the small ribosomal subunit.…”

Section: Discussionsupporting

confidence: 93%

A Genome-Wide Map of Mitochondrial DNA Recombination in Yeast

et al. 2014

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In eukaryotic cells, the production of cellular energy requires close interplay between nuclear and mitochondrial genomes. The mitochondrial genome is essential in that it encodes several genes involved in oxidative phosphorylation. Each cell contains several mitochondrial genome copies and mitochondrial DNA recombination is a widespread process occurring in plants, fungi, protists, and invertebrates. Saccharomyces cerevisiae has proved to be an excellent model to dissect mitochondrial biology. Several studies have focused on DNA recombination in this organelle, yet mostly relied on reporter genes or artificial systems. However, no complete mitochondrial recombination map has been released for any eukaryote so far. In the present work, we sequenced pools of diploids originating from a cross between two different S. cerevisiae strains to detect recombination events. This strategy allowed us to generate the first genome-wide map of recombination for yeast mitochondrial DNA. We demonstrated that recombination events are enriched in specific hotspots preferentially localized in non-protein-coding regions. Additionally, comparison of the recombination profiles of two different crosses showed that the genetic background affects hotspot localization and recombination rates. Finally, to gain insights into the mechanisms involved in mitochondrial recombination, we assessed the impact of individual depletion of four genes previously associated with this process. Deletion of NTG1 and MGT1 did not substantially influence the recombination landscape, alluding to the potential presence of additional regulatory factors. Our findings also revealed the loss of large mitochondrial DNA regions in the absence of MHR1, suggesting a pivotal role for Mhr1 in mitochondrial genome maintenance during mating. This study provides a comprehensive overview of mitochondrial DNA recombination in yeast and thus paves the way for future mechanistic studies of mitochondrial recombination and genome maintenance.

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Section: Discussionsupporting

confidence: 93%

A Genome-Wide Map of Mitochondrial DNA Recombination in Yeast

et al. 2014

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“…The var1 gene (or called rps3 in non-yeast fungi) is another well-studied example of unidirectional inheritance in S. cerevisiae ( Strausberg and Butow, 1981 ). The var1 gene is polymorphic, and different forms of the var1 gene differ by in-frame insertions of short GC-rich palindromic cluster (GC-cluster) in the coding region ( Hudspeth et al, 1984 ).…”

Section: The Rebirth Of Gc-clustersmentioning

confidence: 99%

From Genome Variation to Molecular Mechanisms: What we Have Learned From Yeast Mitochondrial Genomes?

Hao

2022

Front. Microbiol.

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Analysis of genome variation provides insights into mechanisms in genome evolution. This is increasingly appreciated with the rapid growth of genomic data. Mitochondrial genomes (mitogenomes) are well known to vary substantially in many genomic aspects, such as genome size, sequence context, nucleotide base composition and substitution rate. Such substantial variation makes mitogenomes an excellent model system to study the mechanisms dictating mitogenome variation. Recent sequencing efforts have not only covered a rich number of yeast species but also generated genomes from abundant strains within the same species. The rich yeast genomic data have enabled detailed investigation from genome variation into molecular mechanisms in genome evolution. This mini-review highlights some recent progresses in yeast mitogenome studies.

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“…This protein attracted attention as a mt translational product with very unusual features (see below ; for a review, see [65]). The ar1 gene shows unusual conversion mechanisms as well as divergences in the mt genetic code [2,66,67,68]. The tight association of var1 with the small subunit of the mitoribosome was shown by radioactive labelling of mt translational products and comparison with mt ribosomal constituents [47,48].…”

Section: Identification and Characterization Of Mrpsmentioning

confidence: 99%

Mitochondrial ribosomal proteins (MRPs) of yeast

Graack

Wittmann‐Liebold

1998

Biochemical Journal

107

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Mitochondrial ribosomal proteins (MRPs) are the counterparts in that organelle of the cytoplasmic ribosomal proteins in the host. Although the MRPs fulfil similar functions in protein biosynthesis, they are distinct in number, features and primary structures from the latter. Most progress in the eludication of the properties of individual MRPs, and in the characterization of the corresponding genes, has been made in baker's yeast (Saccharomyces cerevisiae). To date, 50 different MRPs have been determined, although biochemical data and mutational analysis propose a total number which is substantially higher. Surprisingly, only a minority of the MRPs that have been characterized show significant sequence similarities to known ribosomal proteins from other sources, thus limiting the deduction of their functions by simple comparison of amino acid sequences. Further, individual MRPs have been characterized functionally by mutational studies, and the regulation of expression of MRP genes has been described. The interaction of the mitochondrial ribosomes with transcription factors specific for individual mitochondrial mRNAs, and the communication between mitochondria and the nucleus for the co-ordinated expression of ribosomal constituents, are other aspects of current MRP research. Although the mitochondrial translational system is still far from being described completely, the yeast MRP system serves as a model for other organisms, including that of humans.

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Gene conversion at the var1 locus on yeast mitochondrial DNA.

Cited by 25 publications

References 24 publications

A Genome-Wide Map of Mitochondrial DNA Recombination in Yeast

A Genome-Wide Map of Mitochondrial DNA Recombination in Yeast

From Genome Variation to Molecular Mechanisms: What we Have Learned From Yeast Mitochondrial Genomes?

Mitochondrial ribosomal proteins (MRPs) of yeast

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